DOI QR코드

DOI QR Code

Effects of Mixtures of Tween80 and Cellulolytic Enzymes on Nutrient Digestion and Cellulolytic Bacterial Adhesion

  • Hwang, Il Hwan (EASY BIO SYSTEM, INC.) ;
  • Lee, Chan Hee (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Seon Woo (Department of Animal and Avian Sciences, University of Maryland) ;
  • Sung, Ha Guyn (DAEHO CO., LTD.) ;
  • Lee, Se Young (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Lee, Sung Sill (Division of Applied Life Science and IALS, Gyeongsang National University) ;
  • Hong, Hee Ok (Department of Food Service Management and Nutrition, Sangmyung University) ;
  • Kwak, Yong-Chul (CheongJu Feed Mill, Nonghyup Saryo) ;
  • Ha, Jong K. (Research Institute for Agriculture and Life Sciences, Seoul National University)
  • 투고 : 2008.06.12
  • 심사 : 2008.09.13
  • 발행 : 2008.11.01

초록

A series of in vitro and in vivo experiments were conducted to investigate the effects of the mixture of Tween 80 and cellulolytic enzymes (xylanase and cellulase) on total tract nutrient digestibility and rumen cellulolytic bacterial adhesion rates in Holstein steers. Ground timothy hay sprayed with various levels of Tween 80 and cellulolytic enzymes was used as substrates in an in vitro experiment to find out the best combinations for DM degradation. The application level of 2.5% (v/w) Tween 80 and the combination of 5 U xylanase and 2.5 U cellulase per gram of ground timothy hay (DM basis) resulted in the highest in vitro dry matter degradation rate (p<0.05). Feeding the same timothy hay to Holstein steers also improved in vivo nutrient (DM, CP, CF, NDF and ADF) digesibilities compared to non-treated hay (p<0.05). Moreover, Tween 80 and enzyme combination treatment increased total ruminal VFA and concentrations of propionic acid and isovaleric acid with decreased acetate to propionate ratio (p<0.001). However, adhesion rates of Fibrobacter succinogenes and Ruminococcus flavefaciens determined by Real Time PCR were not influenced by the treatment while that of Ruminococcus albus was decreased (p<0.05). The present results indicate that a mixture of Tween 80 and cellulolytic enzymes can improve rumen environment and feed digestibility with variable influence on cellulolytic bacterial adhesion on feed.

키워드

참고문헌

  1. AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Washington, DC.
  2. Baah, J., J. A. Shelford, A. N. Hristov, T. A. McAllister and K. J. Cheng. 2005. Effects of Tween80 and fibrolytic enzymes on ruminal fermentation and digestibility of feeds in Holstein cows. Asian-Aust. J. Anim. Sci. 18:816-824. https://doi.org/10.5713/ajas.2005.816
  3. Beauchemin, K. A. and L. M. Rode. 1996. Use of feed enzymes in ruminant nutrition. In: Animal science research and development-meeting future challenges (Ed. L. M. Rode). pp. 103-131. Minister of Supply and Services Canada, Ottawa, Onario.
  4. Beauchemin, K. A., L. M. Rode and V. J. H. Sewalt. 1995. Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forages. Can. J. Anim. Sci. 75:641-644. https://doi.org/10.4141/cjas95-096
  5. Cheng, K. J., C. W. Forsberg, H. Minato and J. W. Costerton. 1991. Microbial ecology and physiology of feed degradation within the rumen. In: Physiological aspects of digestion and metabolism in ruminants (Ed. T. Tsuda, Y. Sasaki and R. Kawashima). pp. 595-624. Academic Press, Toronto ON.
  6. Erwin, E. S., G. T. Marco and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-1771. https://doi.org/10.3168/jds.S0022-0302(61)89956-6
  7. Erickson, T., J. Borjesson and F. Tjerneld. 2002. Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb. Technol. 31:353-364. https://doi.org/10.1016/S0141-0229(02)00134-5
  8. Feng, P., C. W. Hunt, G. T. Pritchard and W. E. Julien. 1996. Effect of enzyme preparations on in situ and in vitro degradation and in vivo digestive characteristics of mature cool-season grass forage in beef steers. J. Anim. Sci. 74:1349-1357. https://doi.org/10.2527/1996.7461349x
  9. Flint, H. J. and C. W. Forsberg. 1995. Polysaccharide degradation in the rumen-biochemistry and genetics. In: Ruminant physiology: digestion, metabolism, growth and reproduction. XIII International Symposium on Ruminant Physiology. pp. 43-70. Ferdinand Enke, Stuttgart.
  10. Goto, M., H. Bae, S. S. Lee, M. S. Yahaya, S. Karita, K. Wanjae and K. J. Cheng. 2003a. Effects of surfactant Tween 80 on forage degradability and microbial growth on the in vitro rumen mixed and pure cultures. Asian-Aust. J. Anim. Sci. 16:672-676. https://doi.org/10.5713/ajas.2003.672
  11. Goto, M., H. Bae, M. S. Yahaya, S. Karita, W. Kim, J. Baah, K. Sugawara and K. J. Cheng. 2003b. Effects of surfactant Tween 80 on enzymatic accessibility and degradation of Orchardgrass (Dactylis glomerata L.) at different growth stage. Asian-Aust. J. Anim. Sci. 16:83-87. https://doi.org/10.5713/ajas.2003.83
  12. Hristov, A. N., T. A. McAllister and K.-J. Cheng. 1998. Effect of dietary or abomasal supplementation of exogenous polysaccharide-degrading enzyme supplementation on rumen fermentation and nutrient digestibility. J. Anim. Sci. 76:3146-3156. https://doi.org/10.2527/1998.76123146x
  13. Kamande, G. M. 1994. Manipulation of rumen and silage fermentation. Doctoral Thesis, University of British Columbia, Vancouver, BC.
  14. Kim, W., Y. Gamo, M. S. Yahaya, W. Yimiti and M. Goto. 2005. Pretreatment of surfactant Tween 80 and fibrolytic enzyme influencing volatile fatty acid and methane production of a total mixed ration by mixed rumen microorganisms at in vitro. Grassland Sci. 51:145-152. https://doi.org/10.1111/j.1744-697X.2005.00020.x
  15. Kim, C. -H., J. N. Kim, J. K. Ha, S. G. Yun and S. S. Lee. 2004. Effects of dietary addition of surfactant Tween 80 on ruminal fermentation and nutrient digestibility of Hanwoo steers. Asian-Aust. J. Anim. Sci. 17:337-342. https://doi.org/10.5713/ajas.2004.337
  16. Kim, W., Y. Gamo, M. S. Yahaya, W. Yimiti, S. Ogawa, S. Karita and M. Goto. 2006. Effect of Tween 80 on hydrolytic activity and substrate accessibility of carbohydrolase 1 (CBH 1) from Trichoderma viride. Asian-Aust. J. Anim. Sci. 19:684-689. https://doi.org/10.5713/ajas.2006.684
  17. Lee, C. H., H. G. Sung, M. Eslami, S. Y. Lee, J. Y. Song, S. S. Lee and J. K. Ha. 2007. Effects of Tween 80 Pretreatment on dry matter disappearance of rice straw and cellulolytic bacterial adhesion. Asian-Aust. J. Anim. Sci. 20:1397-1401. https://doi.org/10.5713/ajas.2007.1397
  18. Lee, S. J., N. Y. Shin, W. Y. Kim, Y. H. Moon, H. S. Kim, J. K. Ha and S. S. Lee. 2007. The effects of negative- and positive-charged surfactants on in vitro DM digestibility and the growth of ruminal mixed microorganisms. Kor. J. Anim. Sci. Tech. 49:647-656. https://doi.org/10.5187/JAST.2007.49.5.647
  19. Lee, S. S. and J. K. Ha. 2003. Influence of surfactant Tween 80 on the gas production, cellulose digestion and enzyme activities by mixed rumen microorganisms. Asian-Aust. J. Anim. Sci. 16:1151-1157. https://doi.org/10.5713/ajas.2003.1151
  20. Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performance of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115. https://doi.org/10.5713/ajas.2003.104
  21. Lewis, G. E., C. W. Hunt, W. K. Sanchez, R. J. Treacher, G. T. Pritchard and P. Feng. 1996. Effect of direct-fed fibrolytic enzymes on the digestive characteristics of a forage-based diet fed to beef steers. J. Anim. Sci. 74:3020-3028. https://doi.org/10.2527/1996.74123020x
  22. Lewis, G. E., W. K. Sanchez, C. W. Hunt, M. A. Guy, G. T. Pritchard, B. I. Swanson and R. J. Treacher. 1999. Effect of direct-fed fibrolytic enzymes on the lactational performance of dairy cows. J. Dairy Sci. 82:611-617. https://doi.org/10.3168/jds.S0022-0302(99)75274-4
  23. McDougall, E. I. 1948. Studies on ruminant saliva. I. The composition and output of sheep's saliva. Biochem. J. 43:99-109. https://doi.org/10.1042/bj0430099
  24. SAS. 1996. SAS User's Guide (Ver. 7) SAS Inst. Inc., Cary, NC
  25. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  26. Wang, Y., T. A. McAllister, L. M. Rode, K. A. Beauchemin, D. P. Morgavi, V. L. Nsereko, A. D. Iwaasa and W. Yang. 2001. Effect of enzymes supplementation on the ruminal fermentation and microbial protein synthesis in Rusitec. Br. J. Nutr. 85:325-332. https://doi.org/10.1079/BJN2000277
  27. Wang, Y., T. A. McAllister, J. Baah, R. Wilde, K. A. Beauchemin, L. M. Rode, J. A. Shelford, G. M. Kamande and K. J. Cheng. 2003. Effects of Tween 80 on in vitro fermentation of silages and interactive effects of Tween 80, monensin and exogenous fibrolytic enzymes on growth performance by feedlot cattle. Asian-Aust. J. Anim. Sci. 16:968-978. https://doi.org/10.5713/ajas.2003.968
  28. Yang, W. Z., K. A. Beauchemin and L. M. Rode. 1999. Effects of enzyme feed additives on extent of digestion and milk production of lactating dairy cows. J. Dairy Sci. 82:391-403. https://doi.org/10.3168/jds.S0022-0302(99)75245-8

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