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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Bombyx mori protein disulfide isomerase enhances the production of nuecin, an antibacterial protein

  • Goo, Tae-Won (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Sung-Wan (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Choi, Kwang-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kang, Seok-Woo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kwon, Ki-Sang (Department of Anatomy, College of Medicine, Chungnam National University) ;
  • Yu, Kweon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University)
  • Published : 2008.05.31
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Abstract

The insect baculovirus expression vector system (BEVS) is useful for producing biologically active recombinant proteins. However, the overexpressions of foreign proteins using this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we developed a versatile baculovirus expression and secretion system using Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion was found to improve the secretions and antibacterial activities of recombinant nuecin proteins. Thus, we conclude that bPDI gene fusion is a useful addition to BEVS for the large-scale production of bioactive recombinant proteins.

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References

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