Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Stage-specific Expression of Ankyrin and SOCS Box Protein-4 (Asb-4) during Spermatogenesis

  • Kim, Soo-Kyoung (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Rhim, Si Youn (Department of Surgery, Hanyang University) ;
  • Lee, Man Ryul (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Kim, Jong Soo (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Kim, Hyung Jun (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Lee, Dong Ryul (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University) ;
  • Kim, Kye-Seong (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University)
  • Received : 2007.11.05
  • Accepted : 2007.12.13
  • Published : 2008.04.30
⟨ Previous Next ⟩

Abstract

Members of the large family of Asb proteins are ubiquitously expressed in mammalian tissues; however, the roles of individual Asb and their function in the developmental testes have not been reported. In this report, we isolated a murine Asb4 from mouse testis. Northern blot analysis revealed that mAsb-4 was expressed only in testes and produced in a stage-specific manner during spermatogenesis. It was expressed in murine testes beginning in the fourth week after birth and extending into adulthood. Pachytene spermatocytes had the highest level of expression. Interestingly, the human homologue of mAsb-4, ASB-4 (hASB-4) was also expressed in human testis. These results suggest that ASB-4 plays pivotal roles in mammalian testis development and spermatogenesis.

Keywords

Acknowledgement

Supported by : Hanyang University

References

  1. Bellve, A.R., Millette, C.F., Bhatnagar, Y.M., and O'Brien, D.A. (1977). Dissociation of the mouse testis and characterization of isolated spermatogenic cells. J. Histochem. Cytochem. 25, 480-494 https://doi.org/10.1177/25.7.893996
  2. Breeden, L., and Nasmyth, K. (1987). Similarity between cellcycle genes of budding yeast and fission yeast and the Notch gene of Drosophila. Nature 329, 651-654 https://doi.org/10.1038/329651a0
  3. Chung, A.S., Guan, Y.J., Yuan, Z.L., Albina, J.E., and Chin, Y.E. (2005). Ankyrin repeat and SOCS box 3 (ASB3) mediates ubiquitination and degradation of tumor necrosis factor receptor II. Mol. Cell. Biol. 25, 4716-4726 https://doi.org/10.1128/MCB.25.11.4716-4726.2005
  4. Guibal, F.C., Moog-Lutz, C., Smolewski, P., Di, Gioia, Y., Darzynkiewicz, Z., Lutz, P.G., and Cayre, Y.E. (2002). ASB-2 inhibits growth and promotes commitment in myeloid leukemia cells. J. Biol. Chem. 277, 218-224 https://doi.org/10.1074/jbc.M108476200
  5. Hilton, D.J., Richardson, R.T., Alexander, W.S., Viney, E.M., Willson, T.A., Sprigg, N.S., Starr, R., Nicholson, S.E., Metcalf, D., and Nicola, N.A. (1998). Twenty proteins containing a C-terminal SOCS box form five structural classes. Proc. Natl. Acad. Sci. USA 95, 114-119
  6. Kamura, T., Sato, S., Haque, D., Liu, L., Kaelin, W.G., Jr., Conaway, R.C., and Conaway, J.W. (1998). The Elongin BC complex interacts with the conserved SOCS-box motif present in members of the SOCS, ras, WD-40 repeat, and ankyrin repeat families. Genes Dev. 12, 3872-3881 https://doi.org/10.1101/gad.12.24.3872
  7. Kho, K.H., and Inaba, K. (2004). Expression of 17beta-hydroxysteroid dehydrogenease in testis of the ascidian Ciona intestinalis corrected . Mol. Cells 18, 171-176
  8. Kile, B.T., Viney, E.M., Willson, T.A., Brodnicki, T.C., Cancilla, M.R., Herlihy, A.S., Croker, B.A., Baca, M., Nicola, N.A., Hilton, D.J., et al. (2000). Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4. Gene 258, 31-41 https://doi.org/10.1016/S0378-1119(00)00402-9
  9. Kile, B.T., Metcalf, D., Mifsud, S., DiRago, L., Nicola, N.A., Hilton, D.J., and Alexander, W.S. (2001). Functional analysis of Asb-1 using genetic modification in mice. Mol. Cell. Biol. 21, 6189-6197 https://doi.org/10.1128/MCB.21.18.6189-6197.2001
  10. Kim, K.S., Kim, M.S., Kim, S.K., and Baek, K.H. (2004). Murine Asb-17 expression during mouse testis development and spermatogenesis. Zygote 12, 151-156 https://doi.org/10.1017/S0967199404002722
  11. Kisseleva, T., Bhattacharya, S., Braunstein, J., and Schindler, C.W. (2002). Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285, 1-24 https://doi.org/10.1016/S0378-1119(02)00398-0
  12. Krebs, D.L., and Hilton, D.J. (2000). SOCS: physiological suppressors of cytokine signaling. J. Cell Sci. 113 (Pt 16), 2813-2819
  13. Lee, D.R., Kim, K.S., Yang, Y.H., Oh, H.S., Lee, S.H., Chung, T.G., Cho, J.H., Kim, H.J., Yoon, T.K., and Cha, K.Y. (2006). Isolation of male germ stem cell-like cells from testicular tissue of non-obstructive azoospermic patients and differentiation into haploid male germ cells in vitro. Hum. Reprod. 21, 471-476 https://doi.org/10.1093/humrep/dei319
  14. Ochoa, G.C., Slepnev, V.I., Neff, L., Ringstad, N., Takei, K., Daniell, L., Kim, W., Cao, H., McNiven, M., Baron, R., et al. (2000). A functional link between dynamin and the actin cytoskeleton at podosomes. J. Cell Biol. 150, 377-389 https://doi.org/10.1083/jcb.150.2.377
  15. Park, C., Choi, W.S., Kwon, H., and Kwon, Y.K. (2001). Temporal and spatial expression of neurotrophins and their receptors during male germ cell development. Mol. Cells 12, 360-367
  16. Rhee, K., and Wolgemuth, D.J. (2002). Tcp10 promoter-directed expression of the Nek2 gene in mouse meiotic spermatocytes. Mol. Cells 13, 85-90
  17. Romrell, L.J., Bellve, A.R., and Fawcett, D.W. (1976). Separation of mouse spermatogenic cells by sedimentation velocity. a morphological characterization. Dev. Biol. 49, 119-131 https://doi.org/10.1016/0012-1606(76)90262-1
  18. Zhang, J.G., Farley, A., Nicholson, S.E., Willson, T.A., Zugaro, L.M., Simpson, R.J., Moritz, R.L., Cary, D., Richardson, R., Hausmann, G., et al. (1999). The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation. Proc. Natl. Acad. Sci. USA 96, 2071-2076