Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Bong-Han Corpuscles as Possible Stem Cell Niches on the Organ-Surfaces

줄기세포의 가능한 원천으로서의 장기표면 봉한소체

  • Kim, Min-Su (Department of Large/Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida) ;
  • Hong, Ju-Young (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University) ;
  • Hong, Su (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University) ;
  • Lee, Byung-Cheon (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University) ;
  • Nam, Chang-Hoon (MRC Laboratory of Molecular Biology, Cambridge, UK) ;
  • Woo, Hee-Jong (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Dae-In ;
  • Soh, Kwang-Sup (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University)
  • 김민수 (플로리다대학교 수의과대학 대.소동물 임상과학과) ;
  • 홍주영 (서울대학교 물리천문학부 한의학물리연구실) ;
  • 홍수 (서울대학교 물리천문학부 한의학물리연구실) ;
  • 이병천 (서울대학교 물리천문학부 한의학물리연구실) ;
  • 남창훈 (캠브리지 의학연구센터 분자생물학연구소) ;
  • 우희종 (서울대학교 수의과대학 면역학연구) ;
  • 강대인 (대한약침학회) ;
  • 소광섭 (서울대학교 물리천문학부 한의학물리연구실)
  • Published : 2008.03.31
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Abstract

Objectives : Showing that Bong-Han corpuscles(BHC) are suppliers of the stem cells in adulthood, and the Bong-Han ducts(BHD) are transportation routes of stem cells. Methods : BHC and BHD were obtained from the internal organ-surfaces of rats. The sliced BHC and BHD were immunostained with various stem cell markers. Extracellular matrices were also analyzed by immunohistochemistry. Result : The presence of mesenchymal stem cells was confirmed by the expression of Integrin beta 1, Collagen type 1 and Fibronectin. But CD54 was not expressed. The hematopoietic stem cell marker, Thy 1 was strongly expressed. BHDs showed Collagen type 1, Fibronectin, and vWF expression. Conclusion : Both hematopoietic and mesenchymal stem cell markers were expressed strongly in BHC similarly as in bone marrow. An endothelial cell marker(vWF) demonstrated the possibility of the stem cell transportation routes of BHD.

목적 : 봉한소체가 성체줄기세포의 원천이며, 봉한관이 줄기세포 수송로일 가능성을 확인함. 방법 : 쥐의 내부 장기표면에서 봉한소체와 봉한관을 채취했다. 다양한 줄기세포 표지항체를 써서 면역조직학적 분석을 했다. 결과 : mesenchymal 줄기세포에 관한 Integrin ${\beta}1$, Collagen type 1, Fibronectin의 강한 발현을 확인했다. CD54는 발현되지 않았다. 조혈줄기세포에 관련하여 Thy 1의 발현이 있었다. 결론 : 골수조직과 유사하게 mesenchymal과 조혈줄기세포의 표지가 BHC에서 확인되었고, 봉한관에서는 vWF가 발현되어 줄기세포 수송로 가능성을 확인했다.

Keywords

References

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