DOI QR코드

DOI QR Code

Transient activation of the MAP kinase signaling pathway by the forward signaling of EphA4 in PC12 cells

  • Shin, Jong-Dae (The Institute of Natural Science, Sookmyung Women's University) ;
  • Gu, Chang-Kyu (The Institute of Natural Science, Sookmyung Women's University) ;
  • Kim, Ji-Eun (Department of Biological Science, Sookmyung Women's University) ;
  • Park, Soo-Chul (Department of Biological Science, Sookmyung Women's University)
  • 투고 : 2008.04.14
  • 심사 : 2008.05.27
  • 발행 : 2008.06.30

초록

In the present study, we demonstrate that ephrin-A5 is able to induce a transient increase of MAP kinase activity in PC12 cells. However, the effects of ephrin-A5 on the MAP kinase signaling pathway are about three-fold less than that of EGF. In addition, we demonstrate that EphA4 is the only Eph member expressed in PC12 cells, and that tyrosine phosphorylation induced by ephrin-A5 treatment is consistent with the magnitude and longevity of MAP kinase activation. Experiments using the Ras dominant negative mutant N17Ras reveal that Ras plays a pivotal role in ephrin-A5-induced MAP kinase activation in PC12 cells. Importantly, we found that the EphA4 receptor is rapidly internalized by endocytosis upon engagement of ephrin-A5, leading to a subsequent reduction in the MAP kinase activation. Together, these data suggest a novel regulatory mechanism of differential Ras-MAP kinase signaling kineticsexhibited by the forward signaling of EphA4 in PC12 cells.

키워드

참고문헌

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