BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Characterization of two plasma membrane protein 3 genes (PutPMP3) from the alkali grass, Puccinellia tenuiflora, and functional comparison of the rice homologues, OsLti6a/b from rice

  • Chang-Qing, Zhang (College of Agronomy and Biotechnology, China Agricultural University) ;
  • Shunsaku, Nishiuchi (Asian Natural Environmental Science Center (ANESC), the University of Tokyo) ;
  • Shenkui, Liu (Alkali Soil Natural Environmental Science Center, Northeast Forestry University) ;
  • Tetsuo, Takano (Asian Natural Environmental Science Center (ANESC), the University of Tokyo)
  • 투고 : 2007.10.11
  • 심사 : 2008.03.17
  • 발행 : 2008.06.30
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초록

Two full-length cDNAs, PutPMP3-1 and PutPMP3-2, encoding PMP3 family proteins were isolated from Puccinellia tenuiflora, a monocotyledonous halophyte. Expression of both genes was induced by low temperature, salt stress, dehydration, ABA, and $NaHCO_3$. Transcripts of PutPMP3-2 were more strongly induced by these stresses relative to those of PutPMP3-1, particularly under low temperature and dehydration conditions. Expression of PutPMP3-1 and PutPMP3-2 in yeast mutants lacking the PMP3 gene can functionally complement the membrane hyper-polarization and salt sensitivity phenotypes resulting from PMP3 deletion. To compare the functions of PutPMP3-1 and PutPMP3-2, the orthologous genes in rice (OsLti6a and OsLti6b) were isolated. Both OsLti6a and OsLti6b could functionally complement the loss of PMP3 in yeast. PutPMP3-2 and OsLti6a were more effective in reversing membrane hyperpolarization than PutPMP3-1 and OsLti6b. However, the four yeast transformants each showed similar levels of salt tolerance. These results imply that these PMP3 family members don't function identically under different stress tolerance conditions.

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참고문헌

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