BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Protein transduction of an antioxidant enzyme: subcellular localization of superoxide dismutase fusion protein in cells

  • Kim, Dae-Won (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kim, So-Young (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Hwa (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Yeum-Pyo (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Min-Jung (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Jeong, Min-Seop (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Jang, Sang-Ho (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Kil-Soo (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kang, Tae-Cheon (Department of Anatomy and Neurobiology, College of Medicine, Hallym University) ;
  • Won, Moo-Ho (Department of Anatomy and Neurobiology, College of Medicine, Hallym University) ;
  • Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine) ;
  • Kwon, Oh-Shin (Department of Biochemistry, Kyungpook National University) ;
  • Eum, Won-Sik (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo-Young (Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University)
  • 투고 : 2007.12.25
  • 심사 : 2007.12.26
  • 발행 : 2008.02.29
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초록

In protein therapy, it is important for exogenous protein to be delivered into the target subcellular localization. To transduce a therapeutic protein into its specific subcellular localization, we synthesized nuclear localization signal (NLS) and membrane translocation sequence signal (MTS) peptides and produced a genetic in-frame SOD fusion protein. The purified SOD fusion proteins were efficiently transduced into mammalian cells with enzymatic activities. Immunofluorescence and Western blot analysis revealed that the SOD fusion proteins successfully transduced into the nucleus and the cytosol in the cells. The viability of cells treated with paraquat was markedly increased by the transduced fusion proteins. Thus, our results suggest that these peptides should be useful for targeting the specific localization of therapeutic proteins in various human diseases.

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참고문헌

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