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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Expression profile identifies novel genes involved in neuronal differentiation

  • Kim, Jung-Hee (Department of Biological Science, Sookmyung Women's University) ;
  • Lee, Tae-Young (Department of Biological Science, Sookmyung Women's University) ;
  • Yoo, Kyung-Hyun (Department of Biological Science, Sookmyung Women's University) ;
  • Lee, Hyo-Soo (Department of Biological Science, Sookmyung Women's University) ;
  • Cho, Sun-A (Department of Biological Science, Sookmyung Women's University) ;
  • Park, Jong-Hoon (Department of Biological Science, Sookmyung Women's University)
  • Received : 2007.09.05
  • Accepted : 2007.10.25
  • Published : 2008.02.29
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Abstract

In the presence of NGF, PC12 cells extend neuronal processes, cease cell division, become electrically excitable, and undergo several biochemical changes that are detectable in developing sympathetic neurons. We investigated the expression pattern of the apoptosis-related genes at each stage of neuronal differentiation using a cDNA microarray containing 320 apoptosis-related rat genes. By comparing the expression patterns through time-series analysis, we identified candidate genes that appear to regulate neuronal differentiation. Among the candidate genes, HO2 was selected by real-time PCR and Western blot analysis. To identify the roles of selected genes in the stages of neuronal differentiation, transfection of HO2 siRNA in PC12 cells was performed. Down-regulation of HO2 expression causes a reduction in neuronal differentiation in PC12 cells. Our results suggest that the HO2 gene could be related to the regulation of neuronal differentiation levels.

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References

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