한국가시화정보학회지 (Journal of the Korean Society of Visualization)

  • 제6권2호
  • /
  • Pages.45-50
  • /
  • 2008
  • /
  • 1598-8430(pISSN)
  • /
  • 2093-808X(eISSN)
한국가시화정보학회 (The Korean Society of Visualization)
권호 표지
DOI QR코드

DOI QR Code

다공성 매질 표면에서 박테리아의 거동: 확산의 영향

Behavior of Bacteria on the Porous Substrates: Diffusion Effect

  • 조명옥 (국민대학교 기계자동차공학부) ;
  • 조지용 (국민대학교 기계자동차공학부) ;
  • 박은정 (국민대학교 기계자동차공학부) ;
  • 이동희 (국민대학교 기계자동차공학부) ;
  • 이정훈 (서울대학교 나노입자제어기술 연구단) ;
  • 김중경 (국민대학교 기계자동차공학부)
  • 발행 : 2008.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

It has been found that the colony size of bacteria grown on an agar plate decreases with increasing agar gel concentration. Evidenc from recent studies suggests that the bacterial colony dynamics is closely related with the mechanical properties of the substrate. We investigate whether bacterial growth on the agar substrate is controlled mostly by the nutrients' diffusion which is hindered more in porous medium than in solution. The number of bacterial cells in single colonies is found to be inversely correlated with agar concentration. High-resolution live cell imaging at the single bacterium level confirms that the bacterial growth rate is reduced with increasing agar concentration. There is a strong correlation between the slowed diffusion and the reduced number of cells in a high concentration of agar medium.

키워드

참고문헌

  1. Fux, C. A., Stoodley, P., Hall-Stoodley, L. and Costerton, J. W., 2003, "Bacterial Biofilms: A Diagnostic and Therapeutic Challenge," Expert Rev. Antiinfect. Ther., Vol. 1(4), pp. 667-683. https://doi.org/10.1586/14787210.1.4.667
  2. Medilanski, E., Kaufmann, K., Wick, L. Y., Wanner, O. and Harms, H., 2002, "Influence of the Surface Topography of Stainless Steel on Bacterial Adhesion," Biofouling, Vol. 18(3), pp. 193-203. https://doi.org/10.1080/08927010290011370
  3. Teughels, W., Van Assche, N., Sliepen, I. and Quirynen, M., 2006, "Effect of Material Characteristics and/or Surface Topography on Biofilm Development," Clin. Oral Imp. Res., Vol. 17(Suppl. 2), pp. 68-81. https://doi.org/10.1111/j.1600-0501.2006.01353.x
  4. Satriano, C., Messina, G. M. L., Carnazza, S., Guglielmino, S. and Marletta, G., 2006, "Bacterial Adhesion onto Nanopatterned Polymer Surfaces," Mater. Sci. Eng. C, Vol. 26(5-7), pp. 942-946. https://doi.org/10.1016/j.msec.2005.09.096
  5. Lichter, J. A., Thompson, M. T., Delgadillo, M., Nishikawa, T., Rubner, M. F. and Van Vliet, K. J., 2008, "Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria," Biomacromolecules, Vol. 9(6), pp. 1571-1578. https://doi.org/10.1021/bm701430y
  6. Cooper, A. L., Dean, A. C. R., Hinshelwood, C., 1968, "Factors Affecting the Growth of Bacterial Colonies on Agar Plates," Proc. R. Soc. B, Vol. 171(1023), pp. 175-199. https://doi.org/10.1098/rspb.1968.0063
  7. Steager, E. B., Kim, C.-B. and Kim M. J., 2008, "Dynamics of Pattern Formation in Bacterial Swarms," Vol. 20(7), pp. 073601-1-073601-5. https://doi.org/10.1063/1.2953245
  8. DiLuzio, W. R., Turner, L., Mayer, M., Garstecki, P., Weibel, D. B., Berg, H. C. and Whitesides, G. M., 2005, "Escherichia Coli Swim on the Right-hand Side," Nature, Vol. 435(7045), pp. 1271-1274. https://doi.org/10.1038/nature03660
  9. Park, S., Wolanin, P. M., Yuzbashyan, E. A., Lin, H., Darnton, N. C., Stock, J. B., Silberzan, P. and Austin, R., 2003, "Influence of Topology on Bacterial Social Interaction," Vol. 100(24), pp. 13910-13915. https://doi.org/10.1073/pnas.1935975100
  10. Cho, H., Jönsson, H., Campbell, K., Melke, P., Williams, J. W., Jedynak, B., Stevens, A. M., Groisman, A. and Levchenko, A., 2007, "Self-organization in High-density Bacterial Colonies: Efficient Crowd Control," Vol. 5(11), pp. 2614-2623.