Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Behavior of Bacteria on the Porous Substrates: Diffusion Effect

다공성 매질 표면에서 박테리아의 거동: 확산의 영향

  • 조명옥 (국민대학교 기계자동차공학부) ;
  • 조지용 (국민대학교 기계자동차공학부) ;
  • 박은정 (국민대학교 기계자동차공학부) ;
  • 이동희 (국민대학교 기계자동차공학부) ;
  • 이정훈 (서울대학교 나노입자제어기술 연구단) ;
  • 김중경 (국민대학교 기계자동차공학부)
  • Published : 2008.12.31
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Abstract

It has been found that the colony size of bacteria grown on an agar plate decreases with increasing agar gel concentration. Evidenc from recent studies suggests that the bacterial colony dynamics is closely related with the mechanical properties of the substrate. We investigate whether bacterial growth on the agar substrate is controlled mostly by the nutrients' diffusion which is hindered more in porous medium than in solution. The number of bacterial cells in single colonies is found to be inversely correlated with agar concentration. High-resolution live cell imaging at the single bacterium level confirms that the bacterial growth rate is reduced with increasing agar concentration. There is a strong correlation between the slowed diffusion and the reduced number of cells in a high concentration of agar medium.

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References

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