Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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The Effect of Exposure to Mixed Organic Solvents on Lipid Peroxidation in Ship Building Painters

  • Park, Jun-Ho (Department of Preventive Medicine, Institute of Occupational and Environmental Medicine, Yonsei University Wonju College of Medicine) ;
  • Cha, Bong-Suk (Department of Preventive Medicine, Institute of Occupational and Environmental Medicine, Yonsei University Wonju College of Medicine) ;
  • Chang, Sei-Jin (Department of Preventive Medicine, Institute of Occupational and Environmental Medicine, Yonsei University Wonju College of Medicine) ;
  • Koh, Sang-Baek (Department of Preventive Medicine, Institute of Life Long Health, Yonsei University Wonju College of Medicine) ;
  • Eom, Ae-Yong (Department of Nursing, Margaret Prtchard University) ;
  • Lee, Kang-Myeung (Department of Preventive Medicine, Institute of Occupational and Environmental Medicine, Yonsei University Wonju College of Medicine) ;
  • Jung, Min-Ye (Department of Occupational Therapy, College of Health Science, Yonsei University) ;
  • Choi, Hong-Soon (Institute of Occupational and Environmental Health, Kwandong University)
  • Published : 2008.12.31
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Abstract

In the last several years, studies on the association of oxidative stress damage with exposure in the work place have been conducted. Xenobiotics create an imbalance of the homeostasis between oxidant molecules and antioxidant defense. By monitoring oxidative stress biomarkers, information was obtained on damages induced by oxidative stress and the toxicity of xenobiotics. In the present study, a Job Exposure Matrix (JEM) was constructed using the data from the Working Environment Measurement (WEM) of painters in the shipyard industry from the past 3 years to assess the exposure status. Additionally, by measuring the concentration of urinary malondialdehyde (MDA), the effect of lipid peroxidation was examined. The subjects consisted of 68 workers who were exposed to mixed organic solvents in the painting process and 25 non-exposure controls. The exposure indices of the exposure groups were significantly different (sprayer: 0.83, touchup: 0.54, assistant: 0.13, P<0.05). The urinary MDA concentration of the exposure group was 48.60${\pm}$ 39.23 ${\mu}mol$/mol creatinine, which was significantly higher than 18.03${\pm}$16.33 ${\mu}mol$/mol creatinine of the control group (P<0.05). From the multiple regression analysis of urinary MDA, the regression coefficient for exposure grade was statistically significant. In future studies, evaluation of the antioxidant levels of subjects should be performed simultaneously with quantitative exposure measurements.

Keywords

References

  1. Rogers, J. V., Rogers, C. M., Garrett, C. M. & Mc-Dougal, J. N. Gene expression in rat skin induced by irritating chemicals. J Biochem Mol Toxic 17:123-137 (2003) https://doi.org/10.1002/jbt.10079
  2. Mattia, C. J., LeBel, C. P. & Bondy, S. C. Effects of toluene and its metabolites on cerebral reactive oxygen species generation. Biochem Pharmacol 42:879-882 (1991) https://doi.org/10.1016/0006-2952(91)90048-A
  3. Mattia, C. J., LeBel, C. P. & Bondy, S. C. Free radical induction in the brain and liver by products of toluene catabolism. Biochem Pharmacol 46:103-110 (1993) https://doi.org/10.1016/0006-2952(93)90353-X
  4. Beyer, R. E. The participation of coenzyme Q in free radical production and antioxidation. Free Radic Biol Med 8:545-565 (1990) https://doi.org/10.1016/0891-5849(90)90154-B
  5. Gutteridge, J. M. C. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41:1819-1828 (1995)
  6. Burk, R. F. & Ludden, T. M. Exhaled alkanes as indices of in vivo lipid peroxidation. Biochem Pharmacol 38:1029-1032 (1989) https://doi.org/10.1016/0006-2952(89)90244-X
  7. Koh, S. B. et al. The similar exposure group and exposure variation in ship-building painters; Focused on xylene exposure. Korean J Occup Environ Med 13:413-422 (2001)
  8. Reed, J. V. & Harcourt, A. K. The essentials of occupational diseases. Baltimore: C C Thomas, 1941:3-9
  9. Goldberg, M., Kromhout, H. & Guenel, P. Job exposure matrices in industry. Int J Epidemiol 22:s10-15 (1993) https://doi.org/10.1093/ije/22.Supplement_2.S10
  10. Olsen, J. Information bias in case-control studies in occupational health epidemiology (letter). Scand J Soc Med 12:1-2 (1984) https://doi.org/10.1177/140349488401200101
  11. Dewar, R., Siemiatycki, J. & Gerin, M. Loss of statistical power associated with the use of a job-exposure matrix in occupational case-control studies. Appl Occup Environ Hyg 6:508-515 (1991) https://doi.org/10.1080/1047322X.1991.10387920
  12. Chechoway, H., Savitz, D. A. & Heyer, N. J. Assessing the effects of non differential misclassification of exposures in occupational studies. Appl Occup Environ Hyg 6:528-533 (1991) https://doi.org/10.1080/1047322X.1991.10387923
  13. Stewar, W. F. & Correa-Villaseno A. False positive exposure errors and low exposure prevalence in community-based case-control studies. Appl Occup Environ Hyg 6:534-540 (1991) https://doi.org/10.1080/1047322X.1991.10387924
  14. Gerin, M. et al. Development and use of a welding process exposure matrix in a historical prospective study of lung cancer risk in European welders. Int J Epidemiol 22:s22-28 (1993) https://doi.org/10.1093/ije/22.Supplement_2.S22
  15. Nilsson, R. I., Nordlinder, R. G., Tagesson, C., Walles, S. & Jarvholm, B. G. Genotoxic effects in workers exposed to low levels of benzene from gasolin. Am J Ind Med 30:317-324 (1996) https://doi.org/10.1002/(SICI)1097-0274(199609)30:3<317::AID-AJIM10>3.0.CO;2-Z
  16. Tagesson, C., Kallberg, M. & Wingren, G. Urinary malondialdehyde and 8-hydroxydeoxyguanosine as potential markers of oxidative stress in industrial art glass workers. Int Arch Occup Environ Health 69:5-13 (1996) https://doi.org/10.1007/BF02630732
  17. Loft, S., Poulsen, H. E., Vistisen, K. & Knudsen, L. E. Increased urinary excretion of 8-oxo-2'-deoxyguanosine, a biomarker of oxidative DNA damage, in urban bus drivers. Mutat Res 441:11-19 (1999) https://doi.org/10.1016/S1383-5718(99)00034-0
  18. Huang, Y. L. et al. Lipid peroxidation in workers exposed to hexavalent chromium. J Toxico Env Health 56:235-248 (1999) https://doi.org/10.1080/009841099158088
  19. Pilger, A., Germadnik, D., Schaffer, A., Theiler, A. & Pils, P. 8-Hydroxydeoxyguanosine in leukocyte DNA and urine of quartz-exposed workers and patients with silicosis. Int Arch Occup Environ Health 73:305-310 (2000) https://doi.org/10.1007/s004200000117
  20. Wu, M. et al. Urinary excretion of 8-hydroxy-2-deoxyguanosine and 1-hydroxypyrene in coke-oven workers. Environ Mol Mutagen 42:98-105 (2003) https://doi.org/10.1002/em.10176
  21. Korchazhkina, O., Christopher, E. & Stephen, A. S. Measurement by revered-phase HPLC of malondialdehyde in normal human urine following derivatisation with 2,4-dinitrophenylhydrazine. J Chromatogr B 794:353-362 (2003) https://doi.org/10.1016/S1570-0232(03)00495-1
  22. Toraason, M. 8-Hydroxydeoxyguanosine as a biomarker of workplace exposures. Biomarkers 4:3-26 (1999) https://doi.org/10.1080/135475099230967
  23. Kasai, H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine, as a marker of cellular oxidative stress during arcinogenesis. Mutat Res 387:147-163 (1997) https://doi.org/10.1016/S1383-5742(97)00035-5
  24. Scarpelli, A., Miligi, L., Seniori Costantini, A. & Alberghini Maltoni, S. Exposure to solvents in the shoe and leather goods industries. Int J Epidemiol 22:s46-50 (1993) https://doi.org/10.1093/ije/22.Supplement_2.S46
  25. Astrakianakis, G. et al. Job-exposure matrixes and retrospective exposure assessment in the pulp and paper industry. Appl Occup Environ Hyg 13:663-670 (1998) https://doi.org/10.1080/1047322X.1998.10390135