Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium

  • Woo, Seon-Ock (Southern Coastal Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Son, Sung-Hee (Southern Coastal Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Park, Hong-Seog (Genome Research Center, Korea Research Institute of Bioscience and Technology) ;
  • Vulpe, Chris D. (Department of Nutritional Sciences and Toxicology, University of California) ;
  • Ryu, Jae-Chun (Laboratory of Cellular and Molecular Toxicology, Korea Institute of Science and Technology) ;
  • Yum, Seung-Shic (Southern Coastal Environment Research Department, Korea Ocean Research and Development Institute)
  • Published : 2008.12.31
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Abstract

To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes.

Keywords

References

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