Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Differential Gene Expression Profiling in Human Promyelocytic Leukemia Cells Treated with Benzene and Ethylbenzene

  • Sarma, Sailendra Nath (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology) ;
  • Kim, Youn-Jung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology) ;
  • Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology)
  • Published : 2008.12.31
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Abstract

Benzene and ethylbenzene (BE), the volatile organic compounds (VOCs) are common constituents of cleaning and degreasing agents, paints, pesticides, personal care products, gasoline and solvents. VOCs are evaporated at room temperature and most of them exhibit acute and chronic toxicity to human. Chronic exposure of benzene is responsible for myeloid leukemia and also ethylbenzene is also recognized as a possible carcinogen. To evaluate the BE effect on human, whole human genome 35 K oligonucleotide microarray were screened for the identification of the differential expression profiling. We identified 280 up-regulated and 201 down-regulated genes changed by more than 1.5 fold by BE exposure. Functional analysis was carried out by using DAVID bioinformatics software. Clustering of these differentially expressed genes were associated with immune response, cytokine-cytokine receptor interaction, toll-like signaling pathway, small cell lung cancer, immune response, apoptosis, p53 signaling pathway and MAPKKK cascade possibly constituting alternative or subordinate pathways of hematotoxicity and immune toxicity. Gene ontology analysis methods including biological process, cellular components, molecular function and KEGG pathway thus provide a fundamental basis of the molecular pathways through BEs exposure in human lymphoma cells. This may provides a valuable information to do further analysis to explore the mechanism of BE induced hematotoxicity.

Keywords

References

  1. MacDonald, J. A. Evaluating natural attenuation for groundwater cleanup, Environ Sci Technol 34:346-353 (2000) https://doi.org/10.1021/es003359w
  2. Hutchins, S. R., Sewell, G. W., Kovacs, D. A. & Smith, G. A. Biodegradation of aromatic hydrocarbons by aquifer microorganisms under denitrifying conditions, Environ Sci Technol 25:68-76 (1991) https://doi.org/10.1021/es00013a005
  3. Nikodinovic, J. et al. The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. Appl Microbial Biotechnol 80:665-673 (2008) https://doi.org/10.1007/s00253-008-1593-0
  4. US EPA. Reducing risk: Setting priorities and strategies for environmental protection. The report of the Science Advisory Board Relative Risk Reduction Strategies Committee. SAB-EC-90-021. US Environmental Protection Agency, Washington, DC, pp. 26 (1990)
  5. Koren, H. S. & Devlin, R. B. Human upper respiratory tract responses to inhaled pollutants with emphasis on nasal lavage. Ann NY Acad Sci 641:215-224 (1992) https://doi.org/10.1111/j.1749-6632.1992.tb16545.x
  6. Sarma, S. N., Kim, Y. J., Jeon, H. K. & Ryu, J. C. Identification of immune responsive genes on benzene, toluene and o-xylene in jurkat cells using 35 k human oligomicroarray. Mol Cell Toxicol 2:229-235 (2006)
  7. Wieslander, G., Norback, D. & Edling, C. Airway symptoms among house painters in relation to exposure to volatile organic compounds (VOCS)-a longitudinal study. Ann Occup Hyg 41:155-166 (1997) https://doi.org/10.1016/S0003-4878(96)00039-7
  8. Diez, U. et al. Redecoration of apartments promotes obstructive bronchitis in atopy risk infants-results of the LARS-study. Int J Hyg Environ Health 206:173-179 (2003) https://doi.org/10.1078/1438-4639-00218
  9. Herbarth, O. et al. Effect of indoor chemical exposure on the development of allergies in newborns-LARS study. Proc Healthy Build 1:281-286 (2000)
  10. Aprea, C., Strambi, M., Novelli, M. T., Lunghini, L. & Bozzi, N. Biologic monitoring of exposure to organophosphate pesticides in 195 Italian children. Environ Health Perspect 108:521-525 (2000) https://doi.org/10.2307/3454613
  11. Needham, L. L. & Sexton, K. Assessing children's exposure to hazardous environmental chemicals: an overview of selected research challenges and complexities. J Expo Anal Environ Epidemiol 10:611-629 (2000) https://doi.org/10.1038/sj.jea.7500142
  12. Sexton, K. et al. Children's exposure to volatile organic compounds as determined by longitudinal measurements in blood. Environ Health Perspect 113:342-349 (2005) https://doi.org/10.1289/ehp.7412
  13. Kim, Y. J., Song, M. & Ryu, J. C. Gene expression profiling of human bronchial epithelial (BEAS-2B) cells treated with nitrofunrantoin, a pulmonary toxicant. Mol Cell Toxicol 3:222-230 (2007)
  14. Xu, T., Du, L. F. & Zhou, Y. Evaluation of GO-based functional similarity measures using S. cerevisiae protein interaction and expression profile data. BMC Bioinformatics 9:472 (2008) https://doi.org/10.1186/1471-2105-9-472
  15. Volinia, S. et al. GOAL: automated gene ontology analysis of expression profiles. Nucleic Acids Res 32:92-99 (2004) https://doi.org/10.1093/nar/gnh090
  16. Ashburne, M. et al. Gene ontology: tool for the unification of biology. Nat Genet 25:25-29 (2000) https://doi.org/10.1038/75556
  17. Kushida, T., Takagi, T. & Fukuda K. I. Event ontology: A pathway-centric ontology for biological processes. Pac Symp Biocomput 11:152-163 (2006)
  18. Te Pas, M. F. et al. Biochemical pathways analysis of microarray results: regulation of myogenesis in pig. BMC Dev Biol 7:66-80 (2007) https://doi.org/10.1186/1471-213X-7-66
  19. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Meth 65:55-63 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  20. Tusher, V. G., Tibshirani, R. & Chu, G. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA 98:5116-5121 (2001)
  21. Krewski, D. et al. Assessing the health risks of benzene: a report on the benzene state-of-the-science workshop. J Toxicol Environ Health A 61:307-338 (2000) https://doi.org/10.1080/00984100050166325
  22. Lan, Q. et al. Polymorphisms in cytokine and cellular adhesion molecule genes and susceptibility to hematotoxicity among workers exposed to benzene. Cancer Res 65:9574-9581 (2005) https://doi.org/10.1158/0008-5472.CAN-05-1419
  23. Luttrell, L. M. et al. G-protein-coupled receptors and their regulation: activation of the MAP kinase signaling pathway by G-protein-coupled receptors. Adv Second Messenger Phosphoprotein Res 31:263-277 (1997)
  24. Germain, R. N. & Stefanova, I. The dynamics of T cell receptor signaling: complex orchestration and the key roles of tempo and cooperation. Annu Rev Immunol 17:467-522 (1999) https://doi.org/10.1146/annurev.immunol.17.1.467
  25. Kim, B. H. et al. Anti-inflammatory benzene diamine compound inhibited toll-like receptor 4-mediated inducible nitric oxide synthase expression and nuclear factor-kappa B activation. Biol Pharm Bull 5:908-911 (2005)
  26. King, K. L. & Cidlowski, J. A. Cell cycle regulation and apoptosis. Annu Rev Physiol 60:601-617 (1998) https://doi.org/10.1146/annurev.physiol.60.1.601
  27. Kelekar, A. & Thompson, C. B. Bcl-2-family proteins: the role of the BH3 domain in apoptosis. Trends Cell Biol 8:324-330 (1998) https://doi.org/10.1016/S0962-8924(98)01321-X
  28. Pruitt, K. & Der, C. J. Ras and Rho regulation of the cell cycle and oncogenesis. Cancer Lett 171:1-10 (2001) https://doi.org/10.1016/S0304-3835(01)00528-6