Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.)

  • Srinivas, L. (Nuclear Agriculture and Biotechnology Division, Plant Cell Culture Technology Section, Bhabha Atomic Research Centre) ;
  • Sunil Kumar, G.B. (Nuclear Agriculture and Biotechnology Division, Plant Cell Culture Technology Section, Bhabha Atomic Research Centre) ;
  • Ganapathi, T.R. (Nuclear Agriculture and Biotechnology Division, Plant Cell Culture Technology Section, Bhabha Atomic Research Centre) ;
  • Revathi, C.J. (Shantha Biotechnics Limited) ;
  • Bapat, V.A. (Nuclear Agriculture and Biotechnology Division, Plant Cell Culture Technology Section, Bhabha Atomic Research Centre)
  • Received : 2007.07.22
  • Accepted : 2008.01.22
  • Published : 2008.04.30
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Abstract

Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus 's' gene, which encodes surface antigen. Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D.W. was noted in pEFEHER-infiltrated cotyledonary leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report on the expression of hepatitis B surface antigen in tomato.

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