Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
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Genomic Alterations in Korean Laryngeal Squamous Cell Carcinoma： Array-Comparative Genomic Hybridization

한국인 후두 편평 상피 세포암의 유전체 이상분석： Array 비교 유전체 보합법

  • Cho, Yoon-Hee (Department of Otolaryngology, Hongik Hospital) ;
  • Park, Soo-Yeun (Department of Anatomy, School of Medicine, Ewha Womans University) ;
  • Lee, Dong-Wook ;
  • Kim, Han-Su (Department of Otolaryngology, School of Medicine, Ewha Womans University) ;
  • Lee, Ja-Hyun (Department of Otolaryngology, School of Medicine, Ewha Womans University) ;
  • Park, Hae-Sang (Department of Otolaryngology, School of Medicine, Ewha Womans University) ;
  • Chung, Sung-Min (Department of Otolaryngology, School of Medicine, Ewha Womans University)
  • 조윤희 (홍익병원 이비인후과) ;
  • 박수연 (이화여자대학교 의학전문대학원 해부학교실) ;
  • 이동욱 ;
  • 김한수 (이화여자대학교 의학전문대학원 이비인후과학교실) ;
  • 이자현 (이화여자대학교 의학전문대학원 이비인후과학교실) ;
  • 박혜상 (이화여자대학교 의학전문대학원 이비인후과학교실) ;
  • 정성민 (이화여자대학교 의학전문대학원 이비인후과학교실)
  • Published : 2008.11.30
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Abstract

Head and neck squamous cell carcinoma(HNSCC) still has poor outcome, and laryngeal cancer is the most frequent subtype of HNSCC. Therefore, there is a need to develop novel treatments to improve the outcome of patients with HNSCC. It is critical to gain further understanding on the molecular and chromosomal alteration of HNSCC to identify novel therapeutic targets but genetic etiology of squamous cell carcinoma of the larynx is so complex that target genes have not yet been clearly identified. Array based CGH(array-CGH) allows investigation of general changes in target oncogenes and tumor suppressor genes, which should, in turn, lead to a better understanding of the cancer process. In this study, We used genomic wide array-CGH in tissue specimens to map genomic alterations found in laryngeal squamous cell carcinomas. As results, gains of MAP2, EPHA3, EVI1, LOC389174, NAALADL2, USP47, CTDP1, MASP1, AHRR, and KCNQ5, with losses of SRRM1L, ANKRD19, FLJ39303, ZNF141, DSCAM, GPR27, PROK2, ARPP-21, and B3GAT1 were observed frequently in laryngeal squamous cell carcinoma tissue specimens. These data about the patterns of genomic alterations could be a basic step for understanding more detailed genetic events in the carcinogenesis and also provide information for diagnosis and treatment in laryngeal squamous cell carcinoma. The high resolution of array-CGH combined with human genome database would give a chance to find out possible target genes which were gained or lost clones.

Keywords

References

  1. Bishop JM. The molecular genetics of cancer. Science. 1987; 235:305-311 https://doi.org/10.1126/science.3541204
  2. Houldsworth J, Mathew S, Rao PH, Dyomina K, Louie DC, Parsa N, et al. REL proto-oncogene is frequently amplified in extranodal diffuse large cell lymphoma. Blood. 1996;87:25-29
  3. Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, et al. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992;258: 818-821 https://doi.org/10.1126/science.1359641
  4. Kallioniemi OP, Kallionemi A, Piper J, Isola J, Waldman FM, Gray JW, et al. Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors. Genes Chromosome Cancer. 1994;10:231-243 https://doi.org/10.1002/gcc.2870100403
  5. Pinkel D, Segraves R, Sudar D, Clark S, Poole I, Kowbel D, et al. High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nat Genet. 1998;20:207-211 https://doi.org/10.1038/2524
  6. Hui AB, Lo KW, Yin XL, Poon WS, Ng HK. Detection of multiple gene amplifications in glioblastoma multiforme using arraybased comparative genomic hybridization. Lab Invest. 2001;81: 717-723 https://doi.org/10.1038/labinvest.3780280
  7. Liu C, Lin S, Chen Y, Chang K, Chang K. Array-comparative genomic hybridization to detect genomewide changes in microdissected primary and metastatic oral squamous cell carcinomas. Mol Carcinog. 2006;45:721-731 https://doi.org/10.1002/mc.20213
  8. Landis SH, Murray T, Bolden S, Wingo PA. Cancer Statistics, 1998. CA Cancer J Clin. 1998;48:6-29 https://doi.org/10.3322/canjclin.48.1.6
  9. Vokes EE, Kies M, Haraf DJ. Induction chemotherapy followed by concomitant chemoradiotherapy for advanced head and neck cancer: Impact on the natural history of the disease. J Clin Oncol. 1995;13:876-880 https://doi.org/10.1200/JCO.1995.13.4.876
  10. Gollin SM. Chromosomal alterations in squamous cell carcinomas of the head and neck: Window to the biology of disease. Head Neck. 2001;23:238-253 https://doi.org/10.1002/1097-0347(200103)23:3<238::AID-HED1025>3.0.CO;2-H
  11. Choi YW, Choi JS, Zheng LT, Lim YJ, Yoon HK, Kim YH, et al. Comparative genomic hybridization array analysis and real time PCR reveals genomic alterations in squamous cell carcinomas of the lung. Lung Cancer. 2006;14:Epub ahead of print
  12. Sunde JS, Donninger H, Wu K, Johnson ME, Pestell RG, Rose GS, et al. Expression profiling identified altered expression of genes that contribute to the inhibition of transforming growth factor-beta signaling in ovarian cancer. Cancer Res. 2006;66: 8404-8412 https://doi.org/10.1158/0008-5472.CAN-06-0683
  13. Bacher U, Schnittger S, Kern W, Trenn G, Weisser M, Haferlach T, et al. Acute myeloid leukemia (AML) with t(8;21)(q22;q22) relapsing as AML with t(3;21) (q26;q22). Cancer Genet Cytogenet. 2006;168:172-174 https://doi.org/10.1016/j.cancergencyto.2006.02.011
  14. Wood LD, Calhoun ES, Silliman N, Ptak J, Szabo S, Powell SM, et al. Somatic mutations of GUCY2F, EPHa3, and NTRK3 in human cancers. Hum Mutat. 2006;27:1060-1061
  15. Yang X, Liu D, Murray TJ, Mitchell GC, Hesterman EV, Karchner SI, et al. The aryl hydrocarbon receptor constitutively represses c-myc transcription in human mammary tumor cells. Oncogene. 2005;24:7869-7881 https://doi.org/10.1038/sj.onc.1208938
  16. Schlade-Bartusiak K, Stembalska A, Ramsey D. Significant involvement of chromosome 13q deletions in progression of larynx cancer, detected by comparative genomic hybridization. J Appl Genet. 2005;46:407-413
  17. Bockmuhl U, Wolf G, Schmidt S, Schwendel A, Jahnke V, Dietel M, et al. Genomic alterations associated with malignancy in head and neck cancer. Head Neck. 1998;20:145-151 https://doi.org/10.1002/(SICI)1097-0347(199803)20:2<145::AID-HED8>3.0.CO;2-2