Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Comparison of Phytoplankton Chlorophyll-$\alpha$ Extracted with Different Solvents

  • Lee, Yun-Kyoung (Department of Environmental Science, Kangwon National University) ;
  • Shin, Myoung-Sun (Department of Environmental Science, Kangwon National University) ;
  • Jung, Yu-Kyong (Department of Environmental Science, Kangwon National University) ;
  • Jang, Chang-Won (Department of Environmental Science, Kangwon National University) ;
  • Kim, Bom-Chul (Department of Environmental Science, Kangwon National University)
  • Published : 2008.12.31
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Abstract

Algal chlorophyll-$\alpha$ is commonly determined by spectro-photometric method using 90% acetone as solvents. However, acetone has low extraction efficiency without grinding filters, and DMF (dimethyl formamide) was tested for the compatibility with acetone. Chlorophyll-$\alpha$ concentration was determined for samples from 5 reservoirs of different trophic states and phytoplankton composition, using acetone extraction with grinding and DMF without grinding. Chlorophyll-$\alpha$ measured by DMF and Acetone did not show a significant difference when using trichromatic method of UNESCO and Lorenzen, and therefore, DMF can substitute acetone. But when acidification method was applied, they showed significant difference of 8%. It can be concluded that DMF can extract chlorophylls more effectively without grinding and it can be a better alternative for the standard extraction solvent.

Keywords

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