Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Construction of a Biofilter Immobilized with Rhodococcus sp. B261 for Removal of H2S Gas Generated by Livestock

  • Yun, Soon-Il (Food Science & Technology Major, Division of Biotechnology, Chonbuk National University)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

To explore the optimal conditions for the removal of $H_{2}S$ gas by biofiltration, various conditions, including inlet $H_{2}S$ concentration, flow rate, moisture, and cell number, were examined. Heterotrophic bacteria were isolated from the compost of the animal excreta. A strain that effectively removed $H_{2}S$ was selected and identified as Rhodococcus rhodochrous B261 by analysis of its 16S rDNA sequence. A cell number of $10^{7}\;cfu/g^{-}compost$ was sufficient to dominate the microbiota, and an effective removal was observed at $H_{2}S$ gas concentrations below 220 mg/L. The moisture content of 33-38% was suitable for activation of the microbial activity and delaying the desiccation. Higher flow rates resulted in lower removal rates of the $H_{2}S$ gas. Under the conditions of $10^7\;cfu/g^{-}compost$, $H_{2}S$ gas concentrations of 220 mg/L, and moisture content of 33-38%, the inlet $H_{2}S$ gas concentrations of 120 and 400 mg/L were completely removed for 34 and 12 days, respectively. The amount of sulfur removed was $2.99{\times}10^{-9}H_{2}S-S/cell$, which was suggested as the amount of sulfur removed by a single cell. The biofilter consisting of the compost and R. rhodochrous B261 could be suitable for a long-term biofilteration for the removal of $H_{2}S$ and other malodorous compounds.

Keywords

References

  1. Atlas RN and Bartha R (1997) In Microbial Ecology, pp. 309-313, Benjamin/Cummings Science Publishing, Califonia
  2. Cho KS, Zhang L, Hirai M, and Shoda M (1991) Removal characteristics of hydrogen sulfide nd methanethiol by Thiobacillus sp. isolated from peat in biological deodorization. J Ferment Bioeng 71, 44-49 https://doi.org/10.1016/0922-338X(91)90302-W
  3. Cho KS, Hirai M, and Shoda M (1992) Degradation of hydrogen sulfide by Xanthomonas sp. strain DY44 isolated from peat. Appl Environ Microbiol 58, 1183-1189
  4. Cho KS, Ryu HW, and Lee NY (2000) Biological deodorization of hydrogen sulfide using porous lava as a carrier of Thiobacillus thiooxidans. J Biosci Bioeng 90, 25-31 https://doi.org/10.1016/S1389-1723(00)80029-8
  5. Fukumori Y and Yamanaka T (1979) Flavocytochrome c of Chromatium vinosum. J Biochem 85. 1405-1414 https://doi.org/10.1093/oxfordjournals.jbchem.a132467
  6. Furusawa N, Togashi I, Hirai M, Shoda M, and Kubota H (1984) Removal of hydrogen sulfide by a biofilter. J Ferment Bioeng 62, 589-594
  7. Haggard HW, Henderson Y, and Charlton TJ (1922) The influence of hydrogen sulfide upon respiration. Am J Physiol 61, 289-297
  8. Kreig NR and Holt JG (1984) Bergey's Manual of Systematic Bacteriology, 8th ed., Vol. 2. pp.1104-1139, Williams & Wilkins, Baltimore
  9. Leson G and Winer AM (1991) Biofiltration: an innovative air pollution control technology for VOC emissions. Air Waste Manage Assoc 41, 1045-1054 https://doi.org/10.1080/10473289.1991.10466898
  10. Nakada Y and Ohta Y (1997) Removal of hydrogen sulfide by deodorant bacteria Bacillus sp. BN53-1. Hakkoushi 75, 425-431
  11. Nakada Y and Ohta Y (1999) Purification and properties of hydrogen sulfide oxidase from a Bacillus sp. BN53-1. J Biosci Bioeng 87, 452-455 https://doi.org/10.1016/S1389-1723(99)80093-0
  12. Nakamura S and Yoda N (1978) Identification Method for Clinical Bacteria. pp. 105-180, Natani Shouten
  13. Ohta Y and Ikeda M (1978) Deodorization of pig feces by Actinomycetes. Appl Environ Microbiol 36, 487-491
  14. Ohta Y and Kuwada Y (1988) Rapid deodorization of cattle feces by microorganisms. Biol Wastes 24, 227-240 https://doi.org/10.1016/0269-7483(88)90064-X
  15. Ohta Y, Sumida K, and Nakada Y (1997) Purification and properities of a sulfide-oxidizing enzyme from Streptomyces sp. strain SH91. Can J Microbial 43, 1097-1101 https://doi.org/10.1139/m97-157
  16. Ottengraf SPP (1986) Exhaust gas purification, H.J. Rehm, G. Reed (Eds.), VCH Verlagesellschaft, Weinhem. Biotechnology 8, 125-218
  17. Park Y, Cho KS, Hirai M, and Shoda M (1993) Removability of malodorous gases from a night soil treatment plant by a pilot-scale peat biofilter inoculated with Thiobacillus thioparus DW. J Ferment Bioeng 76, 55-59 https://doi.org/10.1016/0922-338X(93)90053-B
  18. Park DH, Cha JM, Ryu HW, Lee GW, Yu EY, Rhee JI, Park JJ, Kim SW, Lee IW, Joe YI, Ryu YW, Hur BK, Park JK, and Park K (2002) Hydrogen sulfide removal ulitilizing immobilized Thiobacillus sp. IW with Ca-alginate beads. Biochem Eng J 11, 167-173 https://doi.org/10.1016/S1369-703X(02)00021-9
  19. Shoda M (1991) Methods for the biological treatment of exhaust gas, pp. 31-46, In Biological Degradation of Wastes, Elservier Science Publishers, NY
  20. Shinabe K, Oketani S, Ochi T, and Matsumura M (1995) Characteristics of hydrogen sulfide removal by Thiobacillus thiooxidans KS1 isolated from a carrier-packed biological deodorization system. J Ferment Bioeng 80, 592-598 https://doi.org/10.1016/0922-338X(96)87737-3
  21. Smet E, Chasaya G, Van Langenhove H, and Vertraete W (1996) The effect of inoculation and the type of carrier material used on the biofiltration of methyl sulfides. Appl Microbiol Biotechnol 45, 293-298 https://doi.org/10.1007/s002530050686
  22. Tachihana, T. (1985) New Experimental Science 9. Analytical Chemistry [I]. p. 211, Maruzen Inc. Co
  23. Van Groenestijn JW and Hesselink PGM (1993) Biotechniques for air pollution control. Biodegradation 4, 283-301 https://doi.org/10.1007/BF00695975
  24. Wani AH, Branion RMR, and Lau AK (1997) Biofiltration: a promising and cost-effective control technology for odors, VOCs, and air toxics. J Environ Sci Health A32, 2027-2055
  25. Yun SI and Ohta Y (1998) Removal of gaseous n-valeric acid in the air by Rhodococcus sp. B261 immobilized onto ceramic beads. World J Microbiol Biotechnol 14, 343-348 https://doi.org/10.1023/A:1008805009604
  26. Zhang L, Hirai H, and Shoda M (1991) Removal characteristics of dimethyl sulfide, methanethiol and hydrogen sulfide by Hyphomicrobium sp. 155 isolated from peat biofilter. J Ferment Bioeng 72, 392-396 https://doi.org/10.1016/0922-338X(91)90093-V