Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial Susceptibility Pattern of Enterococcus faecalis and E. faecium from Fish Farms in the Southern Coast of Korea

남해안 어류양식장에서 분리 된 Enterococcus Faecalis와 E. faecium의 항균제 감수성 비교

  • 오은경 (국립수산과학원 식품안전연구과) ;
  • 손광태 (국립수산과학원 남해수산연구소 증식연구과) ;
  • 유홍식 (국립수산과학원 식품안전연구과) ;
  • 김지회 (국립수산과학원 남해수산연구소 양식연구센터) ;
  • 이태식 (국립수산과학원 남해수산연구소 양식연구센터) ;
  • 이희정 (국립수산과학원 식품안전연구과)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The antimicrobial resistance of 160 strains of Enterococcus faecalis and 173 strains of E. faecium to 12 antimicrobial agents was investigated. The test strains were isolated from 126 wild seawater and farmed fish, including olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major), and sea bass (Lateolabrax japonicus), in 2005 and 2006. Overall, 91.9% of the E. faecalis isolates and 88.4% of the E. faecium isolates showed antimicrobial resistance to at least one antimicrobial agent. The pattern of antimicrobial resistance of the isolates differed little according to the species of fish. The percentage of E. faecalis and E. faecium with specific antimicrobial resistance differed according to the sample source. For the isolates from farmed fish samples, 66.7% of E. faecalis were tetracycline resistant and 54.5% of E. faecium were erythromycin resistant. By contrast, in the wild fish seawater samples, 92.0% of E. faecalis were rifampin resistant and 88.5% of E. faecium were tetracycline resistant.

Keywords

References

  1. Acar, J.F. and F.W. Goldstein. 1991. Disk Susceptibility Test. In : Antibiotics in Laboratory Medicine (Lorian, V. ed.). Williams & Wilkins, Baltimore, 17-52
  2. Chang, D.S. and W.K. Choe. Bacteriological studies on market sea foods. 1. Sanitary indicative bacteria in slices of raw fish. 1973b. Bull. Kor. Fish. Soc., 6, 92-96
  3. Chang, D.S. and W.K. Choe. Bacteriological studies on market sea foods. 1. Sanitary indicative bacteria in sundried sea foods. 1973a. Bull. Kor. Fish. Soc., 6, 87-91
  4. Chang, D.S., W.K. Choe and K.O. Cho. Bacteriological studies on market sea foods. 1. Sanitary indicative bacteria in frozen sea foods. 1975. Bull. Kor. Fish. Soc., 8, 157-165
  5. Devriese, L.A., M.D. Collins and R. Wirth. 1992. The genus Enterococcus. In: The Prokaryotes. 2 Ed. (Balows, A., H.G. Truper, M. Dworkin, W. Harder and K.-H. Schleifer eds.) Springer-Verlag, New York, 1465-1481
  6. Gin, A.S. and G.G. Zhanel. 1996. Vancomycin-resistant enterococci. Ann. Pharmacother., 30, 615-624 https://doi.org/10.1177/106002809603000610
  7. Hanes, N.B. and C. Fragala. 1967. Effect of seawater concentration on the survival of indicator bacteria. J. Wat. Poll. Cont. Fed., 39, 97
  8. Heo, J.H., M.H. Jung, M.H. Cho, G.H. Kim, K.C. Lee, J.H. Kim and T.S. Jung. 2002. The study on fish disease with reference to bacterial susceptibility to antibiotics in the southern area of Kyeongnam. J. Vet. Clin., 19, 19-22
  9. Herwig, R.P., J.P. Gray and D.P. Weston. 1997. Anti- bacterial resistant bacteria in surficial sediments near salmon net-cage farms in Puget Sound, Washington. Aquaculture, 149, 263-283 https://doi.org/10.1016/S0044-8486(96)01455-X
  10. Kim, A.R., Y.M. Cho, S.K. Lim, M. Her, W.S. Jeong, S.C. Jung and J.H. Kown. 2007. Antimicrobial resistance of commensal bacteria isolated from food producing animals. 3. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from chicken faecal samples. Kor. J. Vet. Publ. Hlth., 31, 41-49
  11. Kuhn, I., A. Iversen, L.G. Burman, B.O. Liljequist, A. Franklin, M. Finn, F. Aarestrup, A.M. Seyfarth, A.R. Blanch, X. Vilanova, H. Taylor, J. Caplin, M.A. Moreno, L. Dominguez, I.A. Herrero and R. Mollby. 2003. Comparison of enterococcal population in animals, humans, and the environment-a European study. Int. J. Food Micro., 88, 133-145 https://doi.org/10.1016/S0168-1605(03)00176-4
  12. Lee, Y.J., A.R. Kim, S.C. Jung, S.W. Song and J.H. Kim. 2005. Antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus isolated from chicken feces. Korean J. Vet. Res., 45, 163-168
  13. Lee, Y.W., J.H. Kim, S.G. Park and K.M. Lee. 1996. Distribution of indictor organisms in commercial fish and shellfish and influence of storage temperature and period. J. Food Hyg. Safety, 11, 57-70
  14. Lim, S.K., H.S. Lee, J.R. Byun, S.Y. Park and S.C. Jung. 2007a. Antimicrobial resistance of commensal bacteria isolated from food producing animals. 1. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from cattle faecal samples. Kor. J. Vet. Publ. Hlth., 31, 21-29
  15. Lim, S.K., H.S. Lee, J.R. Byun, S.Y. Park and S.C. Jung. 2007b. Antimicrobial resistance of commensal bacteria isolated from food producing animals. 1. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from pig faecal samples. Kor. J. Vet. Publ. Hlth., 31, 31-39
  16. Lynette, M., M. Johnston and L.A. Jaykus. 2004. Anti- microbial Resistance of Enterococcus Species Isolated from Produce. Appl. Environ. Microbiol., 70, 3133- 3137 https://doi.org/10.1128/AEM.70.5.3133-3137.2004
  17. Mundy, L.M., D.F. Sahm and M. Gilmore. 2000. Relation- ship between enterococcal virulence and antimicrobial resistance. Clin. Microbiol. Rev., 13, 513- 522 https://doi.org/10.1128/CMR.13.4.513-522.2000
  18. NCCLS. 2004. Performance standards for antimicrobial susceptibility testing. NCCLS document M100-S14. National committee for clinical laboratory standards, Wayne, PA
  19. Petersen, A., J.S. Andersen, T. Kaewmak, T. Somsiri and A. Dalsgaard. 2002. Impact of integrated fish farming on antimicrobial resistance in a pond environment. Microbiol., 68, 6036-6042
  20. Ramotar, K., W. Woods, L. Larocque and B. Toye. 2000. Comparison of Phenotypic Methods to Identify Enterococci Intrinsically Resistant to Vancomycin. Diagn. Microbiol. Infect. Dis., 36, 119-124 https://doi.org/10.1016/S0732-8893(99)00126-1
  21. Samuelson, O.B., V. Torsvik and A. Ervik. 1992. Long range changes in oxytetracycline concentration and bacterial resistance toward oxytetracycline in a fish farm sediment after medication. Sci. Total Environ., 114, 25-36 https://doi.org/10.1016/0048-9697(92)90411-K
  22. Son, K.T., E.G. Oh, T.S. Lee, H.J. Lee, P.H. Kim and J.H. Kim. 2005a. Antimicrobial susceptibility of Vibrio parahaemomlyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J. Kor. Fish. Soc., 38, 365-371 https://doi.org/10.5657/kfas.2005.38.6.365
  23. Son, K.T., E.G. Oh, T.S. Lee, H.J. Lee, P.H. Kim and J.H. Kim. 2005b. Survey of sanitary indicative bac- teria and pathogenic bacteria in fish farms on the southern coast of Korea. J. Kor. Fish. Soc., 38, 359- 364 https://doi.org/10.5657/kfas.2005.38.6.359
  24. Tailor, S.A.N., E.M. Bailey and M.J. Rybak. 1993. En- terococcus, an emerging pathogen. Ann. Pharmacother., 27, 1231-1242 https://doi.org/10.1177/106002809302701014
  25. Tendencia, E.A. and L.D. De la Pena. 2001. Antibiotic resistance of bacteria from shrimp ponds. Aquaculture, 195, 193-204 https://doi.org/10.1016/S0044-8486(00)00570-6

Cited by

  1. Prevalence and Antimicrobial Resistance of Enterococus faecalis and Enterococcus faecium Isolated from Beef, Pork, Chicken and Sashimi vol.33, pp.1, 2013, https://doi.org/10.5851/kosfa.2013.33.1.133
  2. Monitoring of Antimicrobial Resistance and Genetic Analysis of Enterococcus spp. Isolated from Beef, Pork, Chicken and Fish in Korea vol.29, pp.3, 2014, https://doi.org/10.13103/JFHS.2014.29.3.228
  3. Antimicrobial Resistance in Escherichia coli Isolated from the Shellfish Farms in the Southern Coast of Korea vol.46, pp.5, 2013, https://doi.org/10.5657/KFAS.2013.0528
  4. 낙동강에서 엔테로코커스의 분포특성 및 항생제별 내성률 vol.46, pp.3, 2008, https://doi.org/10.11614/ksl.2013.46.3.360