DOI QR코드

DOI QR Code

Viruliferous Rate of the Small Brown Planthopper in the Maize Field Infected with Rice black-streaked dwarf virus

옥수수 재배지의 벼검은줄오갈병 매개충인 애멸구의 보독충율

  • 이봉춘 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정지훈 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 김정인 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 윤영남 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 홍성준 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 곽도연 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 홍연규 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 강항원 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이기운 (경북대학교)
  • Published : 2008.12.01

Abstract

The Rice black-streaked dwarf virus (RBSDV) infected 99-100% of a $1000\;m^2$-maize field in Mungyeong City in 2007. Adjacent to the area is a Persimmon orchard where barley crops were grown under the trees as green manure crops and for soil amendments. The barley acted as winter host to the small brown plant hoppers (SBPH) enabling the insects to survive and pass the winter season. The existence of RBSDV was detected and confirmed by RT-PCR using S9 specific primer. Samples of the insect vector SBPH were collected in the area on May 3, June 7 and, August 4 and the results of the RT-PCR analysis revealed viruliferous insect rates of 2.9, 4.8, and 4.4%, respectively. These observed viruliferous insect rates were similar with those detected in RBSDV infected rice fields.

2007년 경북문경의 옥수수 재배지 약 $1000\;m^2$에 RBSDV가 약 $99{\sim}100%$ 발생되었다. 이 포장은 바로 인접지역에 감나무가 재배되고 있었으며 이 나무 아래에 녹비작물로서 보리를 재배하였다. 보리는 RBSDV의 매개충인 애멸구의 겨울철 중간기주로 작용하여 애멸구의 월동에 유리한 환경으로 작용한 것으로 생각된다. 진단은 S9 특이 primer를 사용한 RT-PCR 방법으로 확인하였다. 이 포장에서 매개충인 애멸구를 채집하여 RT-PCR에 의한 보독충율을 조사하였다. 5월 3일, 6월 7일, 8월 4일 3회 애멸구를 채집하여 검정한 결과 각각 2.9%, 4.8%, 4.4%의 보독충율을 나타내었다. 이것은 RBSDV에 이병된 벼 포장에서 나타나는 보독충율과 비슷한 결과를 나타내었다.

Keywords

References

  1. Lee, B. C., Hong, Y. K., Hong, S. J., Park, S. T. and Lee, K. W. 2005. Occurrence and detection of rice black-streaked dwarf virus in Korea. Plant Pathol. J. 21: 172-173 https://doi.org/10.5423/PPJ.2005.21.2.172
  2. Lee, M. H., Hey, C. H., Park, K. B. and Lee, Y. S. 1989. Productivity of silage by chemical control under different planting date in the Rice black-streaked dwarf virus of corn. Res. Rept. RDA 31: 31-35
  3. Fang, S., Yu, J., Feng, J., Han, C., Li, D. and Liu, Y. 2001. Identification of Rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Arch Virol. 146: 167-170 https://doi.org/10.1007/s007050170200
  4. Isogai, M., Uyeda, I. and Choi, J. K. 2001. Molecular diagnosis of Rice black-streaked dwarf virus in Japan and Korea. Plant Pathol. J. 17: 164-168
  5. Shikata, E. and Kitagawa, Y. 1977. Rice black-streaked dwarf virus: Its properties, morphology, and intracellular localization. Virology 77: 826-842 https://doi.org/10.1016/0042-6822(77)90502-5
  6. Uyeda, I., Lee, B.-C. and Ando, Y. 1998. Reovirus isolation and RNA extraction. In : Foster GD, Taylor SC (eds). Methods in Mol. Biol. 81: 65-75
  7. Wang, Z. H., Fang, S. G., Xu, J. L., Sun, L. Y., Li, D. W. and Yu, J. L. 2003. Sequence analysis of the complete genome of Rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes 27: 163-168 https://doi.org/10.1023/A:1025776527286
  8. Zhang, H. M., Chen, J. P. and Adams, M. J. 2001. Molecular characterization of segments 1 to 6 of Rice black-streaked dwarf virus from China, provides the complete genome. Arch Virol. 146: 2331-2339 https://doi.org/10.1007/s007050170006

Cited by

  1. Agronomic Characteristics and Yield of Silage Corn Hybrids 'Cap 444NG' vol.32, pp.1, 2012, https://doi.org/10.5333/KGFS.2012.32.1.9
  2. Selection of Silage Corn Hybrids for Paddy Field in Chungnam Region vol.31, pp.4, 2011, https://doi.org/10.5333/KGFS.2011.31.4.353