Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison of two diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR to discriminate between Salmonella enterica serovars Gallinarum and S Pullorum

Salmonella enterica serovars Gallinarum과 S Pullorum의 감별을 위한 2가지 진단법: allele-specific real-time PCR과 3'-tailed PCR의 비교

  • Lee, Se-Mi (BK21 Biotherapy Human Resources Center) ;
  • Seo, Ja-Young (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jae-Il (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Tae-Jung (BK21 Biotherapy Human Resources Center)
  • 이세미 (BK21 바이오치료인력양성팀) ;
  • 서자영 (전남대학교 수의과대학) ;
  • 이재일 (전남대학교 수의과대학) ;
  • 김태중 (BK21 바이오치료인력양성팀)
  • Published : 2008.11.30
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Abstract

Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR over 2 single nucleotide polymorphisms ($237^{th}\;and\;598^{th}$). In both methods, $237^{th}$ allele was found to be a good target for differential diagnosis, while $598^{th}$ allele produced some non-specific reactions.

Keywords

References

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