Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Laboratory and Field Evaluations of Entomopathogenic Lecanicillium attenuatum CNU-23 for Control of Green Peach Aphid (Myzus persicae)

  • Kim, Hyang-Yeon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Hyang-Burm (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Young-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, In-Seon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

An entomopathogenic fungus was isolated from an infected aphid. The isolate conformed most closely to Lecanicillium attenuatum CBS 402.78 (AJ292434) based on the internal transcribed spacer (ITS) region of its 18S rDNA, and thus was designated L. attenuatum CNU-23. Laboratory and field evaluations of CNU-23 blastospores were carried out for the control of green peach aphids. The laboratory evaluations of CNU-23 revealed an aphid mortality of about 80% with an estimated $LT_{50}$ of 3.72 days after the application of CNU-23 at $1{\times}10^6$ blastospores/ml. Meanwhile, the field evaluations of CNU-23 performed on greenhouse pepper plants during the rainy season showed an aphid mortality ranging from 72% to 97%. Significant sporulation was observed in the aphids treated with CNU-23. Therefore, the results suggest that L. attenuatum CNU-23 can be used as a biocontrol agent for green peach aphids on greenhouse pepper plants.

Keywords

References

  1. Askary, H., N. Benhamou, and J. Brodeur. 1999. Ultrastructural and cytochemical characterization of aphid invasion by the Hyphomycete Verticillium lecanii. J. Invertebr. Pathol. 74: 1-13 https://doi.org/10.1006/jipa.1999.4857
  2. Faria, M. and S. P. Wraight. 2001. Biological control of Bemisia tabaci with fungi. Crop Prot. 20: 767-778 https://doi.org/10.1016/S0261-2194(01)00110-7
  3. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791 https://doi.org/10.2307/2408678
  4. Fournier, V. and J. Brodeur. 2000. Dose-response susceptibility of pest aphids (Homoptera: Aphididae) and their control of hydroponically grown lettuce with the entomopathogenic fungus Verticillium lecanii, azaridactin, and insecticidal soap. Environ. Entomol. 29: 568-578 https://doi.org/10.1603/0046-225X-29.3.568
  5. Goettel, M. S., R. J. St. Leber, N. W. Rizzo, D. C. Staples, and D. W. Roberts. 1989. Ultrastructural localization of a cuticledegrading protease produced by the entomopathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexa) cuticle. J. Gen. Microbiol. 135: 2233-2239
  6. Jackson, C. W., J. B. Heale, and R. A. Hall. 1985. Traits associated with virulence to the aphid Macrosiphoniella sanborni in eighteen isolates of Verticillium lecanii. Ann. Appl. Biol. 106: 39-48 https://doi.org/10.1111/j.1744-7348.1985.tb03092.x
  7. Kim, J. J., M. S. Goettel, and D. R. Gillespie. 2007. Potential of Lecanicillium species for dual microbial control of aphids and the cucumber powdery mildew fungus, Sphaerotheca fuliginea. Biol. Control 40: 327-332 https://doi.org/10.1016/j.biocontrol.2006.12.002
  8. Lee, H. B., J. Y. Park, and H. S. Jung. 2005. Identification, growth and pathogenicity of Colletotrichum boninense causing leaf anthracnose on Japanese spindle tree. Plant Pathol. J. 21: 27-32 https://doi.org/10.5423/PPJ.2005.21.1.027
  9. Lee, H. B., J. Y. Park, H. S. Jung, and R. C. Summerbell. 2006. Phaeomoniella zymoides and Phaeomoniella pinifoliorum spp. nov., new acid-tolerant epiphytic fungi isolated from pine needles in Korea. Mycologia 98: 598-611 https://doi.org/10.3852/mycologia.98.4.598
  10. Lee, H. B., K. M. Kim, and H. S. Jung. 2005. Paraphaeosphaeria recurvifoliae, a new species causing leaf spots and necrosis on Yucca recurvifolia. Fungal Diver. 20: 71-81
  11. Lopez-Llorca, L. V., T. Carbonell, and J. Salinas. 1999. Colonization of plant waste substrates by entomopathogenic and mycoparasitic fungi: A SEM study. Micron 30: 325-333 https://doi.org/10.1016/S0968-4328(99)00031-1
  12. Lopez-Llorca, L. V. and T. Carbonell. 1998. Use of almond mesocarp for production of the entomopathogenic fungus Verticillium lecanii. Can. J. Microbiol. 44: 886-895 https://doi.org/10.1139/cjm-44-9-886
  13. Marshall, R. K., M. T. Lester, T. R. Glare, and J. T. Christeller. 2003. The fungus, Lecanicillium muscarium, is an entomopathogen of passionvine hopper (Scolypopa australis). N. Z. J. Crop Hort. Sci. 31: 1-7 https://doi.org/10.1080/01140671.2003.9514229
  14. Matsumoto, Y., G. Saucedo-Castaneda, S. Revah, and K. Shirai. 2004. Production of $\beta$-N-acetylhexosaminidase of Verticillium lecanii by solid state and submerged fermentation utilizing shrimp waste silage as substrate and inducer. Process Biochem. 39: 665-671 https://doi.org/10.1016/S0032-9592(03)00140-7
  15. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425
  16. St. Leger, R. J., R. M. Cooper, and A. K. Charnley. 1986. Cuticle-degrading enzymes of entomopathogenic fungi: Cuticle degradation in vitro by enzymes from entomopathogens. J. Invertebr. Pathol. 47: 167-177 https://doi.org/10.1016/0022-2011(86)90043-1
  17. Sun, M. and X. Z. Liu. 2006. Carbon requirements of some nematophagous, entomopathogenic and mycoparasitic hyphomycetes as fungal biocontrol agents. Mycopathologia 161: 295-205 https://doi.org/10.1007/s11046-006-0249-9
  18. Wekesa, V. W., M. Knapp, N. K. Maniania, and H. I. Boga. 2006. Effects of Beauveria bassiana and Metarhizium anisopliae on mortality, fecundity and egg fertility of Tetranychus evansi. J. Appl. Entomol. 130: 155-159 https://doi.org/10.1111/j.1439-0418.2006.01043.x
  19. White, T. J., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. A. Innis, D. H. Gelfand, and J. J. Sninsky (eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, London, U.K
  20. Yoon, C.-S., G.-H. Sung, H.-S. Park, S.-G. Lee, and J.-O. Lee. 1999. Potential of the entomopathogenic fungus, Beauveria bassiana strain CS-1, as a biocontrol agent of Plutella xylostella (Lep., Yponomeutidae). J. Appl. Entomol. 123: 423-425 https://doi.org/10.1046/j.1439-0418.1999.00389.x
  21. Zare, R. and W. Gams. 2001. A revision of Verticillium section Prostrata. The genera Lecanicillium and Simplicillium. Nova Hedwigia 73: 1-50

Cited by

  1. Control of Green Peach Aphid (Myzus Persicae) by Combination of Plant Oil Formulations and Low-dosed Imidacloprid vol.29, pp.3, 2008, https://doi.org/10.5338/kjea.2010.29.3.239
  2. Beauveria bassiana Bb08의 살충성 물질 생산을 위한 배양조건의 통계적 최적화 vol.41, pp.4, 2008, https://doi.org/10.4014/kjmb.1309.09006
  3. Morphological, molecular and virulence characterization of three Lencanicillium species infecting Asian citrus psyllids in Huangyan citrus groves vol.125, pp.None, 2008, https://doi.org/10.1016/j.jip.2015.01.002