The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-atherosclerosis Effect of Imyosan Extract in Human Aortic Smooth Muscle Cells

사람 대동맥 평활근 세포에서 이묘산(二妙散)의 항동맥경화 활성

  • Yun, Hyun-Jeong (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University) ;
  • Heo, Sook-Kyoung (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University) ;
  • Yi, Hyo-Seung (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University) ;
  • Kim, Tae-Hoon (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Dong-Wan (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Sun-Mo (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University)
  • 윤현정 (동국대학교 한의과대학 심혈관계질환 천연물 연구개발센터) ;
  • 허숙경 (동국대학교 한의과대학 심혈관계질환 천연물 연구개발센터) ;
  • 이효승 (동국대학교 한의과대학 심혈관계질환 천연물 연구개발센터) ;
  • 김태훈 (동국대학교 한의과대학 방제학교실) ;
  • 김동완 (동국대학교 한의과대학 방제학교실) ;
  • 김선모 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 심혈관계질환 천연물 연구개발센터)
  • Published : 2008.12.30
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Abstract

Objectives: This study was evaluated to elucidate the inhibitory potential of Imyosan(IMS) and its components, Phellodendri Cortex(PC: Phellodendron amurense Rupr., Hwangbaek in Korean) and Atractylodis Rhizoma(AR: Atratylodes lancea D.C., Changchool in Korean), on human aortic smooth muscle cells(HASMC) migration and production of matrix metalloproteinase (MMP)-2 and MMP-9 by TNF-${\alpha}$ treatment. Methods: Cytotoxic activity of IMS and its components on HASMC was using 5-(3-caroboxy meth-oxyphenyl)-2H-tetra-zolium inner salt(MTS) assay. Effect of IMS, PC and AR on TNF-${\alpha}$-induced HASMC migration underside of matrigel filter was stained with hematoxylin-eosin. And total number of cells that migrated to the underside of the filter was counted. MMP-2 and MMP-9 activity was evaluated by gelatin zymography assay. Results: The matrigel migration assay showed that IMS effectively inhibited the TNF-${\alpha}$-induced migration of HASMC. Moreover, IMS significantly inhibited MMP-9 activity. Our present study demonstrates that IMS and its components inhibit TNF-${\alpha}$-induced HASMC migration and MMP-9 activity. The inhibitory effect of IMS extract is more potent than that of its component herb extracts. Conclusions: These results provide evidence that IMS has multiple effects in the inhibition of HASMC migration and may offer a therapeutic approach to block HASMC migration.

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