북강활 에틸아세테이트분획의 비만세포에서의 염증반응 억제효과

The Ethylacetate Extract of North Kangwhal(Ostericum koreanum) Attenuates the Inflammatory Responses in PMA/A23187-stimulated Mast Cells

  • 서운교 (동국대학교 분당한방병원 내과) ;
  • 이주일 (동국대학교 분당한방병원 내과) ;
  • 박준홍 (봉화고랭지약초시험장) ;
  • 박용기 (동국대학교 한의과대학 한방신약개발센터.한의학연구소)
  • Seo, Un-Kyo (Department of Internal Medicine, Dongguk University Oriental Hospital) ;
  • Lee, Ju-Il (Department of Internal Medicine, Dongguk University Oriental Hospital) ;
  • Park, Jun-Hong (Bongwha Alpine Medicinal Plant Experiment Station) ;
  • Park, Yong-Ki (Oriental Medicine Drug R&D Center and Oriental Medicine Research Institute, College of Oriental Medicine, Dongguk University)
  • 발행 : 2008.12.30

초록

Objectives: In this study, the pharmacological effects of the ethylacetate extract of Ostericum koreanum(North Kangwhal; NK) on allergic inflammation were investigated in activated human mast cells. Methods: North Kangwhal was extracted with 80% methanol for 24 h, and then fractionated with ethylacetate(NK-EtOAc extract). HMC-1 cells, an human mast line, were pre-incubated with different concentrations of NK-EtOAc extract for 30 min, and then stimulated with PMA(50 nM/ml) and A23187($1{\mu}M/ml$) at indicated times. The cell toxicity was determined by MTT assay. The concentrations of prostaglandin E2(PGE2) and cytokines(TNF-${\alpha}$, IL-8) were measured by enzyme-linked immunosorbant assay. Results: NK-EtOAc extract($10{\sim}50{\mu}g/ml$) significantly inhibited the productions of $PGE_2$, TNF-${\alpha}$ and IL-8 in PMA/A23187-stimulated HMC-1 cells without cell toxicity($0{\sim}50{\mu}g/ml$). NK-EtOAc extract also inhibited PMA/A23187-induced phosphorylation of ERK1/2 MAPK and the NF-${\kappa}B$ p65 subunit translocation into the nuclear of HMC-1 cells. Conclusions: This study suggests that NK-EtOAc extract may have an anti-inflammatory property through suppressing the production of inflammatory mediators in activated mast cells and its molecular mechanism underlies the blocking of NF-${\kappa}B$ pathway.

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