Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Conversion of Ginsenosides by 9 Repetitive Steamings and Dryings Process of Korean Ginseng Root and Its Inhibition of BACE-1 Activity

인삼의 구증구포에 의한 Ginsenoside의 성분변화 및 BACE-1 억제효과

  • Kim, Do-Wan (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Lee, Yun-Jin (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Min, Jin-Woo (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Se-Young (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
  • 김도완 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 김유진 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 이연진 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 민진우 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 김세영 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 양덕춘 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행)
  • Published : 2008.12.25
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Abstract

Red ginseng possibly has new ingredients converted during steaming and dry process from fresh ginseng. Kujeungkupo method which means 9 repetitive steamings and dryings process was used for the production of red ginseng from 6-year old ginseng roots. Saponin was extracted from each red ginseng produced at the 1st, 3rd, 5th, 7th, and 9th during the steaming and drying treatment, and we analyzed saponin content with TLC. Minor saponins, such as ginsenoside-Rg3, -Rh2, compound K, and F2, increased as the process time of steaming and drying, but major saponins (ginsenoside-Rb1, -Rb2, -Rc, -Rd, -Re, -Rf, -Rg1) were decreased. Major saponins were yet observed almost at the 1st process, then degraded as the increasing time of steaming and drying process. Especially, ginsenoside-Re and -Rg were observed as considerable amount after the 1st treatment, but there were no trace of them after the 9th treatment. Ginsenoside-Rg1, -Rb2, and -Rb1 were also reduced remarkedly by 96.6%, 96%, and 92.3%, respectively. Minor saponins were increased significantly, especially for ginsenoside-Rg3 and ginsenoside-F2. These results suggest that Kujeungkupo method is the very useful method for the production of minor ginsenoside-Rg3 and -Rh2.

Keywords

References

  1. Shibata, S., Tanaka, T., Ando, T., Sado, M., Tsushima, S., Ohsawa, T. Chemical studies on oriental plant drugs (XIV). Protopanaxadiol a genuine sapogenin of ginseng saponin. Chem Pharm Bull. 14: 595-600, 1966. https://doi.org/10.1248/cpb.14.595
  2. 박채규, 전병선, 양재원. 고려인삼의 화학성분. 식품산업과 영양. 8: 10-23, 2003.
  3. Sato, T., Kojima, S., Toda, S., Arich, S. Effects of ginseng saponin on experimental gastric ulcer (1). Yakuyouyakuriz zasshi. 20: 715-722, 1980.
  4. Kumar, A. Chemopreventive action of ginseng on DMBA-induced papilloma genesis in the skin of mice. Proc 6th Int'l Ginseng Symp Korea Ginseng Tobacco Research Institute. pp 66-73, 1980.
  5. Kenarova, B., Neychev, H., Hadjiivanova, C., Petkov, V.D. Immunomodulating activity of ginsenosides Rg1 from Panax ginseng. Japan J Pharmacol. 54: 447-454, 1990. https://doi.org/10.1254/jjp.54.447
  6. Singh, V.K., Agarwal, S.S., Gupta, B.M. Immunomodulatory activity of Panax ginseng extract. Proc 4th Int'l Ginseng Symp. Korea Ginseng & Tobacco Research Institute. pp 225-232, 1984.
  7. Zhang, J.T., Qu, Z.W., Liu, Y., Deng, H.L. Preliminary study on antiamnestic mechanism of ginsenoside Rg1 and Rb1. Clin Med J. 103: 932-938, 1990.
  8. Joo, C.N. Some physiological and biochemical aspects of saponin fraction of Panax ginseng C. A. Meyer. Korean J Ginseng Sci. 14: 143-156, 1990.
  9. Song, J.H., Park, M.J., Kim, E., Kim, Y.C. Effects of Panax ginseng on galactosamine-induced cytotoxicity in primary cultured rat hepatocytes. Yakhak Hoeji. 34: 341-347, 1990.
  10. Kang, S.Y., Kim, S.H., Schini, V.B., Kim, N.D. Dietary ginsenosides endothelium dependent relaxation in the thoracic arorta of hypercholesterolemic rabbit. Gen Pharmacol. 26: 83-487, 1995.
  11. Chung, Y.H., Kim, K.W., Oura, H. Effects of ginsenoside Rb2 on the anti-oxidants in senescence-accelerated mice (SAM-R/1). Proceeding of the 6th Int'l Symposium Korea Ginseng & Tobacco Research Institute. pp 30-32, 1993.
  12. 김낙두. 홍삼의 약리작용. J Ginseng Res. 25: 2-10, 2001.
  13. 남기열. 홍삼과 백삼의 비교 고찰. J Ginseng Res. 29: 1-18, 2005. https://doi.org/10.5142/JGR.2005.29.1.001
  14. Sinha, S., Anderson, J.P., Barbour, R., Basi, G.S., Caccavello, R., Davis, D., Doan, M. et al. Purification and cloning of amyloid precursor protein $\beta$-secretase from human brain. Nature. 402(6761):537-540, 1999. https://doi.org/10.1038/990114
  15. Lin, X., Koelsch, G., Wu, S., Downs, D., Dashti, A., Tang, J. Human aspartic protease memapsin 2 cleaves the $\beta$-amyloid precursor protein. Proc Natl Acad Sci USA. 97(4):1456-1460, 2000.
  16. 마진열, 하창수, 성형제, 지옥표. Cyclophosphamide로 Rat에 유도된 악성빈혈에 대한 숙지황의 증(蒸)수에 따른 치료효능에 관한 연구. Kor J Pharmacogn. 31: 325-334, 2000.
  17. 김도완, 이연진, 민진우, 이범수, 인준교, 양덕춘. 인삼의 구증구포(九蒸九曝)에 의한 갈변물질, 조지방, 조단백 및 향기성분의 변화. 동의생리병리학회지 22(2):333-339, 2008.
  18. Jeon, S.Y., Bae, K., Seong, Y.H., Song, K.S. Green tea catechins as a. BACE-1 ($\beta$-Secretase) inhibitor. Bioorg Med Chem Lett. 13(22):3905-3908, 2003. https://doi.org/10.1016/j.bmcl.2003.09.018
  19. 고성권, 이충렬, 최용의, 임병옥, 성종환, 윤광로. 백삼 및 홍삼 농축액의 사포닌분석. 한국식품과학회지 35: 536-539, 2003.
  20. Mochizuki, M., Yoo, Y.C., Matsuzawa, K., Sato, K., Saiki, I., Tono-Oka, S., Samukawa, K. et al. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-Rb2, 20(R)- and 20(S)-ginsenoside-Rg3, of red ginseng. Biol Pharm Bull. 18: 1197-1202, 1995. https://doi.org/10.1248/bpb.18.1197
  21. Nam, K.Y. The comparative understading between red ginseng and white ginsengs processed ginsengs (Panax ginseng C. A. Meyer). J Ginseng Res. 29: 1-18, 2005. https://doi.org/10.5142/JGR.2005.29.1.001
  22. Park, J.D. Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C. A. Meyer). Korean J Ginseng Sci. 20: 389-396, 1996.