Reproductive and Developmental Biology

  • 제32권4호
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  • Pages.249-253
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  • 2008
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

In Vitro Development of Interspecies Nuclear Transfer Embryos using Porcine Oocytes with Goat and Rabbit Somatic Cells

  • Quan, Yan Shi (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Naruse, Kenji (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Choi, Su-Min (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kim, Myung-Youn (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Han, Rong-Xun (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Park, Chang-Sik (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Jin, Dong-Il (Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
  • 발행 : 2008.12.31
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초록

Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro-matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM-199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1kV/cm for $30{\mu}s$ 0.3M mannitol medium. The activated cloned embryos were cultured in porcine zygote medium-3 (PZM-3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos reconstructed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to the morular stage than those cultured in PZM-3. These results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine-porcine cloned embryos and that culture condition could be an important factor in iSCNT. The lower developmental potential of goat-porcine and porcine-bovine cloned embryos may be due to incompatibility between the porcine oocyte cytoplasm and goat and bovine somatic nuclei.

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참고문헌

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