Reproductive and Developmental Biology

  • 제32권4호
  • /
  • Pages.245-248
  • /
  • 2008
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Effects of Viability of Canine Oocytes Vitrified-Warmed by the EDS and EDT Methods

  • Park, Ji-Hoon (College of Veterinary Medicine, Chungnam National University) ;
  • Abe, Yasuyuki (Obihiro University of Agriculture and Veterinary Medicine) ;
  • Kim, Sang-Keun (College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2008.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In the present studies, we have intended to compare the EDS (20% EG + 20% DMSO + 0.4 M sucrose + 10% FCS) and EDT (20% EG + 20% DMSO + 0.3 M trehalose 10% FCS) methods for vitrification of canine oocytes, in order to improve the vitrification methods. The survival rate of vitrified-thawed oocytes using the EDS method was $15.1{\pm}1.8%$ (p<0.05), which was lower than that of the control group $(66.7{\pm}2.5%)$. About $45{\sim}55%$ of the vitrified-warmed oocytes showed normal morphology, as assessed by PI staining. However, the ratio of survival rate of oocytes showed lower than that of normal morphology in comparison between EDS method and control group. The survival and developmental rates of vitrified-warmed oocytes by the EDS and EDT methods were $16.7{\pm}1.4%\;and\;11.1{\pm}0.8%$ and $8.3{\pm}1.4%\;and\;4.4{\pm}1.8%$, respectively (p<0.05). The results were significantly lower than the control group $(66.7{\pm}2.5%\;and\;16.7{\pm}3.7%)$. However, the survival rate of vitrified-warmed oocytes using EDS method showed higher than that in the ETS group.

키워드

참고문헌

  1. Abe Y, Hara K, Matsumoto H, Kobayashi J, Sasada H, Ekwall H, Rodriguez-Martinez H, Sato E (2005): Feasibility of a nylon-mesh holder for vitrification of bovine germinal vesicle oocytes in subsequent production of viable blastocysts. Biol Reprod 72:1416-1420 https://doi.org/10.1095/biolreprod.104.037051
  2. Abe Y, Lee DS, Kim SK, Suzuki H (2008): Vitrification of canine oocytes. J Mamm Ova Res 25:32-36 https://doi.org/10.1274/jmor.25.32
  3. Clark P, Rahy GM, Karow Jr AM (1984): Factor influencing renal cryopreservation. II. Toxic effects of three cryopreservation in combination with three vehicle solutions in non-frozen rabbit cortical slices. Cryobiology 21:260-273 https://doi.org/10.1016/0011-2240(84)90322-5
  4. Cuello C, Sntonia MG, Parrilla I, Tornel J, Vazquez JM, Roca J, Berthelot F, Martinat-Botte F, Martinez EA (2004): Vitrification of porcine embryos at various development stages using different ultra-rapid cooling procedures. Theriogenology 62:353-361 https://doi.org/10.1016/j.theriogenology.2003.10.007
  5. Fahy GM, MacFarlane DR, Angell CA, Meryman HT (1984): Vitrification as an approach to cryopreservation. Cryobiology 21:407-426 https://doi.org/10.1016/0011-2240(84)90079-8
  6. Fujihira T, Kishida R, Fukui Y (2004): Developmental capacity of vitrified immature porcine oocytes following ICSI: Effects of cytochalasin B and cryoprotectants. Cryobiology 49:286-290 https://doi.org/10.1016/j.cryobiol.2004.08.004
  7. Hewitt DA, England GCW (1997): The canine oocyte penetration assay its use as an indicator of the spermatozoal performance in vitro. Anim Reprod Sci 50:123-139 https://doi.org/10.1016/S0378-4320(97)00083-3
  8. Hewitt DA, England GCW (1999): Synthetic oviductal fluid and oviductal cell coculture for canine oocytes maturation in vitro. Anim Reprod Sci 55(1):63-75 https://doi.org/10.1016/S0378-4320(98)00162-6
  9. Isachenko V, Soler C, Isachenko E, Perez-Sanchez F, Grishchenko V (1998): Vitrification of immature porcine oocytes: Effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant. Cryobiology 36:250- 253 https://doi.org/10.1006/cryo.1998.2079
  10. Ishijima T, Kobayashi Y, Lee DS, Ueta YY, Matsui M, Lee JY, Suwa Y, Miyahara K, Suzuki H (2006): Cryopreservation of canine ovaries by vitrification. J Reprod Dev 52:293-299 https://doi.org/10.1262/jrd.17080
  11. Iwasaki S, Yoshikane Y, Watanabe S, Nakahara T (1994): Effect of freezing of bovine preimplantation embryos derived from oocytes fertilized in vitro on survival of their inner cells. Mol Reprod Dev 37:272-275 https://doi.org/10.1002/mrd.1080370305
  12. Kasai M, Komi JH, Takakamo A, Tssnoda H, Sakurai T, Machida T (1991): A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 89:91-97
  13. Lee BK, Rhee MH, Kim SK (2008): Effects of cryoprotectants on in vitro development of vitrified immature porcine oocytes following ICSI. J Emb Trans 23(1):77-80
  14. Massip A, Mermilod P, Wilis C, Dessy F (1993): Effects of dilution procedure and culture conditions after thawing on survival of frozen bovine blastocysts produced in vitro. J Reprod Fert 97:65-69 https://doi.org/10.1530/jrf.0.0970065
  15. Otoi T, Shin T, Kraemer DC, Westhusin ME (2004): Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization. Reprod Nutr Dev 44:631-637 https://doi.org/10.1051/rnd:2004065
  16. Otoi T, Shimizu R, Naoi H, Wongsrikeao P, Agung B, Taniguchi M (2005): Meiotic competence of canine oocytes embedded in collagen gel. Reprod Domest Anim 41:17-21 https://doi.org/10.1111/j.1439-0531.2006.00639.x
  17. Rodrigues BA, Carboneiro dos Santos L, Rodrigues JL (2004): Embryonic development of in vitro matured and in vitro fertilized dog oocytes. Mol Reprod Dev 67:215-223 https://doi.org/10.1002/mrd.10394
  18. Saha S, Otoi T, Suzuki T (1996): The efficiency of ethylene glycol, trehalose and polyvinylpyrrolidone for successful vitrification of IVF bovine embryos. J Reprod Dev 42:163-169 https://doi.org/10.1262/jrd.42.163
  19. Saint-Dizier M, Renard JP, Chastant-Maillard S (2001): Induction of final maturation by sperm penetration in canine oocytes. Reproduction 121:97-105 https://doi.org/10.1530/rep.0.1210097
  20. Saito N, Imai K, Tomizawa M (1994): Effect of sugar-addition on the survival of vitrified bovine blastocysts produced in vitro. Theriogenology 41:1053-1063 https://doi.org/10.1016/S0093-691X(05)80028-7
  21. Songsasen N, Yu I, Leibo SP (2002): Nuclear maturation of canine oocytes cultured in protein-free media. Mol Reprod Dev 62:407-415 https://doi.org/10.1002/mrd.10130
  22. Sutton RL (1992): Critical cooling rates for aqueous cryoprotectants in the presence of sugars and polysaccharides. Cryobiology 29:585-598 https://doi.org/10.1016/0011-2240(92)90063-8
  23. Utsumi K, Hochi S, Iritani A (1982): Cryopretective effect of polyols on rat embryos during two-step freezing. Cryobiology 29:332-341 https://doi.org/10.1016/0011-2240(92)90034-Y
  24. Voelkel SA, Hu YX (1992) use of ethylene glycol as a cryoprotectant for bovine embryos allowing direct transfer of frozen thawed embryos to recipient females. Theriogenology 37:687-697 https://doi.org/10.1016/0093-691X(92)90148-K