Journal of Plant Biotechnology

  • 제35권4호
  • /
  • Pages.337-343
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  • 2008
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Expression of Arabidiopsis CAX4 in tomato fruits increases calcium level with no accumulation of other metallic cations

  • Jeong, Se-Woon (Division of Plant Biosciences, Kyungpook National University) ;
  • Han, Jeung-Sul (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Kyung-Min (Division of Biological Environment Science, Kyungpook National University) ;
  • Oh, Jung-Youl (Division of Biological Environment Science, Kyungpook National University) ;
  • Kim, Byung-Oh (Division of Biological Environment Science, Kyungpook National University) ;
  • Kim, Chang-Kil (Division of Biological Environment Science, Kyungpook National University) ;
  • Chung, Jae-Dong (Division of Plant Biosciences, Kyungpook National University)
  • 발행 : 2008.12.31
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초록

We generated transgenic tomato plants with Arabidopsis thaliana $H^+$/cation exchanger gene (C4X4) by Agrobactrium-mediated transformation. We confirmed transgene copy number and transcription by Southern and Northern blot analyses. The intact CAX4-expressing tomato (Lycopersicon esculentum) fruits contained 63-71% more calcium ($Ca^{2+}$) than wild-type fruits. Moreover, ectopic expression of C4X4 in tomato fruits did not show any significant increase of the four kinds of metallic cations analyzed ($Mg^{2+}$, $Fe^{2+}$, $Mn^{2+}$, and $Cu^{2+})$. The C4X4-expressing tomato plants including their fruits did not show any morphological alternations during whole growth period. These results suggest the enhanced Ca-substrate specificity of CAX4 exchanger in tomato. Therefore, intact CAX4 exchanger can be a useful tool for $Ca^{2+}$ nutrient enrichment of tomato fruits with reduced accumulation of undesirable cations.

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참고문헌

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