Enterobacteriaceae and Related Microorganisms Isolated from Rump of Raw Beefs

  • Kwon, Eun-Ah (School of Food Technology and Food Service Industry, College of Natural Resources, Yeungnam University) ;
  • Kim, Myung-Hee (School of Food Technology and Food Service Industry, College of Natural Resources, Yeungnam University)
  • 발행 : 2008.12.31

초록

In this study, 50 rump samples of raw beef obtained from local Korean supermarkets were analyzed to survey microbial distributions. As results, mesophilic microorganisms ranged from $(1.4{\pm}0.01){\times}10^2$ to $(1.6{\pm}0.05){\times}10^5\;CFU/g$, and total coliforms ranged from 0 to $(1.3{\pm}0.04){\times}10^4\;CFU/g$. Major foodborne pathogens, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp., were not found among the samples. However, Staphylococcus aureus was isolated with 4% frequency. Other isolated microorganisms included Enterobacter amnigenus (4%), Enterobacter cloacae (24%), E. coli (24%), Listeria innocua (8%), Staphylococcus saprophyticus (56%), Staphylococcus xylosus (10%), and Staphylococcus warneri (8%).

키워드

참고문헌

  1. Hughes C, Gillespie IA, O'Brien SJ, The Breakdowns in Food Safety Group. Foodborne transmission of infectious intestinal disease in England and Wales, 1992-2003. Food Control 18: 766-772 (2007) https://doi.org/10.1016/j.foodcont.2006.01.009
  2. Lee WC, Sakai T, Lee MJ, Hamakawa M, Lee SM, Lee IM. An epidemiological study of food poisoning in Korea and Japan. Int. J. Food Microbiol. 29: 141-148 (1996) https://doi.org/10.1016/0168-1605(95)00075-5
  3. Korea Food & Drug Administration. 2005. Outbreak food poisoning. Available from: http://fm.kfda.go.kr/. Accessed June 10, 2007
  4. Korea Food & Drug Administration. 2005. KFDA news. Available from http://www.kfda.go.kr/. Accessed June 10, 2007
  5. Korea Foods Industry Association. Application of criterion and standard. pp. 58-59. In: Korean Food Standards Codex. Moonyoungsa, Seoul, Korea (2006)
  6. Reid CA, Small A, Avery SM, Buncic S. Presence of food-borne pathogens on cattle hides. Food Control 13: 411-415 (2002) https://doi.org/10.1016/S0956-7135(01)00050-0
  7. Elder RO, Keen JE, Siragusa GR, Barkocy-Gallagher GA, Koohmaraie M, Laegreid WW. Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. P. Natl. Acad. Sci. USA 97: 2999-3003 (2000)
  8. Jung HO, Kim ES, Park BL. Raw fish (meat) dish. pp. 334-335. In: Traditional Food of Korean. La YC (ed). Kyojisa, Seoul, Korea (1999)
  9. Korea Foods Industry Association. Methods of microbial experiment. pp. 660-694. In: Korean Food Standards Codex. Moonyoungsa, Seoul, Korea (2006)
  10. Codex Alimentarius Commission. Principles for the establishment and application of microbiological criteria for foods. CAC/GL 21. Food and Agriculture Organization of the United Nations, Rome, Italy (1997)
  11. Rompre A, Servais P, Baudart J, de-Roubin MR, Laurent P. Detection and enumeration of coliforms in drinking water: Current methods and emerging approaches. J. Microbiol. Meth. 49: 31-54 (2002) https://doi.org/10.1016/S0167-7012(01)00351-7
  12. Kim HJ, Nam KJ, Lee DS, Paik HD. Distributions of microorganisms and identification of pathogenic bacteria isolated in raw beef of jangzorim. Korean J. Food Sci. Technol. 36: 683-687 (2004)
  13. Lee YW, Park SG. Distribution of indicator organisms and influence of storage temperature and period in commercial animal foods. J. Food Hyg. Saf. 13: 430-440 (1998)
  14. Waje C, Kim MY, Nam KC, Jo C, Kim DH, Lee JW, Kwon JH. Effect of irradiation on the color, microbiological quality, and sensory attributes of frozen ground beef, pork, and chicken after 6 months at $−6^{\circ}C$. Food Sci. Biotechnol. 17: 212-215 (2008)
  15. Morse EV, Duncan MA. Salmonellosis-An environmental health problem. J. Am. Vet. Med. Assoc. 165: 1015-1019 (1974)
  16. Andrews WH, Hammack TS. Salmonella. Available from U.S. FDA/CFSAN BAM (http://www.cfsan.fda.gov/~ebam/bam-5.html). Updated August 8, 2006. Accessed June 20, 2007
  17. Hajna AA. Triple-sugar iron agar medium for the identification of the intestinal group of bacteria. J. Bacteriol. 49: 516-517 (1945)
  18. Richard C. The genus Enterobacter. pp. 465-467. In: Bergey's Manual Systematic Bacteriology. Sneath PHA, Holt JG (eds). Williams and Wilkins, Baltimore, MD, USA (1986)
  19. Lin YC, Chen TL, Ju HL, Chen HS, Wang FD, Yu KW, Liu CY. Clinical characteristics and risk factors for attributable mortality in Enterobacter cloacae bacteremia. J. Microbiol. Immunol. Infect. 39: 67-72 (2006)
  20. Hitosugi M, Takatsu A, Kawato H, Kai A. An autopsy case of food poisoning due to Staphylococcus aureus. Leg. Med. 3: 123-124 (2001) https://doi.org/10.1016/S1344-6223(01)00011-6
  21. Gunn BA, Dunkelberg WE, Creitz JR. Clinical evaluation of 2% LSM medium for primary isolation and identification of staphylococci. Am. J. Clin. Pathol. 57: 236-240 (1972) https://doi.org/10.1093/ajcp/57.2.236
  22. Lee S, Kim J, Kim M. Distribution of microorganisms and foodborne pathogens in yukae. Korean J. Food Sci. Anim. Resour. 27: 197-202 (2007) https://doi.org/10.5851/kosfa.2007.27.2.197
  23. Kloos WE, Schleifer KH. The genus Staphylococcus. pp. 1013-1027. In: Bergey's Manual Systematic Bacteriology. Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds). Williams and Wilkins. Baltimore, MD, USA (1986)
  24. Tselenis-Kotsowilis AD, Koliomichalis MP, Papavassiliou JT. Acute pyelonephritis caused by Staphylococcus xylosus. J. Clin. Microbiol. 16: 593-594 (1982)
  25. Kamath U, Singer C, Isenberg HD. Clinical significance of Staphylococcus warneri bacteremia. J. Clin. Microbiol. 30: 261-264 (1992)
  26. Duffy G, Cummins E, Nally P, O'Brien S, Butler F. A review of quantitative microbial risk assessment in the management of Escherichia coli O157:H7 on beef. Meat Sci. 74: 76-88 (2006) https://doi.org/10.1016/j.meatsci.2006.04.011
  27. Feng P, Weagant SD. Diarrheagenic Escherichia coli. Available from U.S. FDA/CFSAN BAM (http://www.cfsan.fda.gov/~ebam/bam-4a.html). Updated December 5, 2006. Accessed June 20, 2007
  28. March SB, Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J. Clin. Microbiol. 23: 869-872 (1986)
  29. Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiol. Rev. 55: 476-511 (1991)
  30. Seeliger HPR, Jones D. The genus Listeria. pp. 1235-1242. In: Bergey's Manual Systematic Bacteriology. Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds). Williams and Wilkins, Baltimore, MD, USA (1986)
  31. Tergney A, Bolton DJ. Validation studies on an online monitoring system for reducing faecal and microbial contamination on beef carcasses. Food Control 17: 378-382 (2006) https://doi.org/10.1016/j.foodcont.2005.01.004