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콩나물 부패균 Pseudomonas sp. SN239 동정과 콩나물 부패병 내병성 계통 선발

Identifications of a Sprout-Rot Pathogen Pseudomonas Species SN239 and Selection Resistant Soybean Line

  • 임종수 (영남대학교 생명공학부 생명공학) ;
  • 도금숙 (영남대학교 생명공학부 생명공학) ;
  • 이동선 (영남대학교 생명공학부 생명공학) ;
  • 강상구 (영남대학교 생명공학부 생명공학) ;
  • 서상곤 (영남대학교 자연자원대학 원예학과) ;
  • 박의호 (영남대학교 생명공학부 생명공학)
  • Lim, Jong-Soo (Biotechnology Major, School of Biotechnology, Yeungnam University) ;
  • Do, Kum-Sook (Biotechnology Major, School of Biotechnology, Yeungnam University) ;
  • Lee, Dong-Sun (Biotechnology Major, School of Biotechnology, Yeungnam University) ;
  • Kang, Sang-Gu (Biotechnology Major, School of Biotechnology, Yeungnam University) ;
  • Suh, Sang-Gon (Department of Horticulture, College of Natural Sciences Yeungnam University) ;
  • Park, Eui-Ho (Biotechnology Major, School of Biotechnology, Yeungnam University)
  • 발행 : 2008.12.30

초록

콩나물은 우리나라에서 오래 전부터 재배하여 온 채소로서 그 기호성이 매우 높으며 영양학적으로 우수하나 일부 열악한 재배환경으로 콩나물의 부패문제가 자주 발생해 왔다. 따라서 본 연구는 시중의 부패된 콩나물로부터 다양한 병원균을 분리함과 동시에 재래콩 유전자원으로부터 시중의 콩나물 부패병에 강한 품종을 탐색하고 선발된 내병성 계통의 생육특성을 조사하였다. 분리된 콩나물 부패균들 중 병원성이 강한 콩나물 부패균인 Pseudomonas sp. SN239을 분리하고 16S rRNA 염기서열을 동정한 결과 P. putita, P. plecoglossicida, P. monteilii 및 P. mevalonii와 근연관계를 보였으나 완전히 일치하지는 않았으므로 Pseudomonas sp. SN239는 새로이 동정된 콩나물 부패균으로 여겨진다. 또한 재래콩 194계통에 콩나물 부패병균 Pseudomonas sp. SN239을 접종하여 저항성을 검정한 결과, 이병성 계통은 심하게 부패되었으나 한국 고유계통 YNPCS3-19는 병원성이 없었으며 또한 지속적으로 생육하였다. 그러므로 부패균 저항성 계통 YNPCS3-19는 부패균 저항성 품종 육성에도 활용 가치가 크다고 판단된다.

Control microbial contamination in pathogens to soy sprouts has always been highly concerned in soybean sprout industries because the soybean sprouts are consumed largely as a nutritious fresh vegetable around the world. However, pathogens in soy sprouts are little known. Here, we isolated a strain of Pseudomonas sp. SN239 that caused severer symptoms in sprouts of many soybean cultivars. In phylogenetic relationships using 16S ribosomal RNA sequences of the Pseudomonas species, the identified Pseudomonas sp. SN239 was grouped with P. putita, P. plecoglossicida, P. monteilii and P. mevalonii. Thus, the bacterial strain SN239 might be a newly identified Pseudomonas species which closely related to P. putida. Furthermore, we found that a Korean indigenous soybean (Glycine max) cultivar YNPCSS3-19 has strong resistance against the Pseudomonas sp. SN239.

키워드

참고문헌

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피인용 문헌

  1. Evaluation of the Resistance of Mungbean Lines to Sprout Rot Caused by Pseudomonas species vol.22, pp.7, 2012, https://doi.org/10.5352/JLS.2012.22.7.987