Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Rheum undulatum Extract on Antioxidant Activity and Activity of Matrix Metalloproteinase-1 in Human Skin Fibroblasts

대황 추출물의 항산화 활성 및 MMP-1 저해 활성

  • Park, Sung-Min (R&D Center, CoSeedBioPharm Corporation) ;
  • Lee, Gye-Won (Department of Pharmaceutical Engineering, Konyang University) ;
  • Cho, Young-Ho (Department of Pharmaceutical Engineering, Konyang University)
  • 박성민 ((주)코씨드바이오팜 기술연구소) ;
  • 이계원 (건양대학교 제약공학과) ;
  • 조영호 (건양대학교 제약공학과)
  • Published : 2008.12.30
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Abstract

Rheum undulatum L. has been commonly used as a cure for hematemesis, dropsy, and haematuria in the Oriental medicine for a long time. The main constituents of R. undulatum are chrysophanol and emodin, which are an antioxidative substance that has an anthraquinone structure. In the present study, to develop a new anti-aging agent, we examined the antioxidant activity and the inhibitory effect of the R. undulatum extract on the synthesis of MMP-1 in UVA-irradiated human dermal fibroblasts and MMP-1 activity. The R. undulatum extract was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide radicals in the xanthine/ xanthine oxidase system by a dose-dependent manner, respectively. UVA-induced MMP-1 expression was reduced about 79.5% by 1 ${\mu}g$/ml of the R. undulatum extract and also inhibited MMP-1 activity in a dose-dependent manner. In conclusion, it was observed that the R. undulatum extract has the antioxidant activity, regulation of UVA-induced MMP-1 production, and inhibition of MMP-1 activity. Therefore, these results suggest that the R. undulatum extract can be developed as a new anti-aging component of cosmetics.

본 연구에서는 대황 추출물에 의한 항산화 효과, MMP-1 활성 저해효과 및 자외선이 조사된 사람 피부 섬유아세포에서 MMP-1 발현에 미치는 영향을 관찰하였다. 대황 추출물의 DPPH radicals과 초산소 음이온 라디칼 소거효과는 농도 의존적인 경향을 나타내었으며, DPPH와 초산소 음이온 라디칼을 각각 20 ${\mu}g$/ml에서 72%와 80% 정도 소거하여 우수한 항산화 효과를 나타내었다. 자외선이 조사된 사람 피부 섬유아세포에서 MMP-1의 발현 저해효과 측정에서 대황 추출물은 1 ${\mu}g$/ml에서 약 79.5%로 매우 우수한 합성 저해효과를 나타내었다. 또한 대황 추출물의 MMP-1 활성 저해효과는 20 ${\mu}g$/ml에서 약 70%의 우수한 활성 저해효과를 나타내었다. 이상의 결과를 종합하여 볼 때 대황 추출물은 항산화 효과 및 MMP-1 활성 저해효과와 자외선에 의한 MMP-1의 발현을 효과적으로 저해하는 것으로 보아 우수한 항노화 소재로써 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0
  2. Brenneisen, P., H. Sies and K. Scharffetter-Kochanek. 2002. Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann. N.Y. Acad. Sci. 973, 31-43. https://doi.org/10.1111/j.1749-6632.2002.tb04602.x
  3. Cadenas, E. 1989. Biochemistry of oxygen toxicity. Annu. Rev. Biochem. 58, 79-110. https://doi.org/10.1146/annurev.bi.58.070189.000455
  4. Cho, Y. H., G. S. Sim, J. H. Kim, S. M. Park, B. C. Lee, H. B. Pyo, Y. P. Yun and H. D. Park. 2004. Effect of Melothria heterophylla extract on expression of matrix metalloproteinase- 1 in human skin fibroblasts. Yakhak Hoeji. 48, 358-363.
  5. Chung, J. H., S. W. Kang, J. Varani, J. Lin, G. J. Fisher and J. J. Voorhees. 2000. Decreased extracellular-signal regulated kinase and increased stress-activated MAP kinase activities in aged human skin in vivo. J. Invest. Dermatol. 115, 177-182. https://doi.org/10.1046/j.1523-1747.2000.00009.x
  6. Davies, K. J. 1987. Protein damage and degradation by oxygen radicals. J. Biol. Chem. 262, 9914-9920.
  7. Furuno, K., T. Akasako and N. Sugihara. 2002. The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids. Biol. Pharm. Bull. 25, 19-23. https://doi.org/10.1248/bpb.25.19
  8. Hatano, T. 1995. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Natural Medicines 49, 357-363.
  9. Jung, H. A., H. Y. Chung, T. Yokozawa, S. K. Kim and J. S. Choi. 2004. Alaternin and emodin with hydroxyl radical inhibitory and/or scavenging activities and hepatoprotective activity on tacrine-induced cytotoxicity in HepG2 cells. Arch. Pharm. Res. 27, 947-953. https://doi.org/10.1007/BF02975849
  10. Kitahara, A., U. Matsumoto, H. Ueda and R. Ueoka. 1992. A remarkable antioxidation effect of natural phenol derivatives on the autoxidation of $\gamma$-irradiated methyl linolate. Chem. Pharm. Bull. 40, 2208-2209. https://doi.org/10.1248/cpb.40.2208
  11. Kochanek, S. K., M. Wlaschek, K. Briviba and H. Sies. 1993. Singlet oxygen induces collagenase expression in human skin fibroblast. FEBS Lett. 331, 304-306. https://doi.org/10.1016/0014-5793(93)80357-Z
  12. Kondo, S. 2000. The roles of cytokines in photoaging. J. Dermatol. Sci. 23, S30-S36. https://doi.org/10.1016/S0923-1811(99)00076-6
  13. Kuppusamy, P. and J. L. Zweier. 1989. Characterization of free radical generation by xanthine oxidase. J. Biol. Chem. 264, 9880-9884.
  14. Lavker, R. M. and A. M. Kligman. 1988. Chronic heliodermatitis: a morphologic evaluation of chronic actinic dermal damage with emphasis on the role of mast cells. J. Invest. Dermatol. 90, 325-330. https://doi.org/10.1111/1523-1747.ep12456193
  15. Masaki, H., S. Sakaki, T. Atsumi and H. Sakurai. 1995. Active oxygen scavenging activity of plant extracts. Biol. Pharm. Bull. 18, 162-166. https://doi.org/10.1248/bpb.18.162
  16. Mosmann, T. 1983. Rapid colorimetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay. J. Immunol. Methods 65, 55-59. https://doi.org/10.1016/0022-1759(83)90303-4
  17. Oh, S. J., N. I. Beak and H. Y. Kim. 2001. Piceatannol, Antioxidant compound isolated from the root of Rheum undulatum L. J. Korean Soc. Agric. Chem. Biotechnol. 44, 208-210.
  18. Park, J. H. 2002. The Encyclopedia of Chinese Crude Drugs. Shinilbooks Publications, Seoul, pp. 180-184.
  19. Shah, C. S., J. S. Qadry and J. G. Bhatt. 1972. Qualitative and quantitative evaluation of anthraquinone derivatives in Indian thubarb. Plant Med. 22, 103-108. https://doi.org/10.1055/s-0028-1099590
  20. Wang, Y., A. R. Johnson, Q. Z. Ye and R. D. Dyer. 1999. Catalytic activities and substrate specificity of the human membrane type 4 matrix metalloproteinase catalytic domain. J. Biol. Chem. 274, 33043-33049. https://doi.org/10.1074/jbc.274.46.33043
  21. Wlaschek, M., K. Brviba, G. P. Stricklin, H. Sies and K. Scharffetter-Kochanek. 1995. Singlet oxygen may mediate the ultraviolet A induced synthesis of interstitial collagenase. J. Invest. Dermatol. 104, 194-198. https://doi.org/10.1111/1523-1747.ep12612751

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