Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Quantitative Trait Loci (QTLs) for Unsaturated Fatty Acid Contents in Soybean Seed Using Recombinant Inbred Lines

콩에서 microsatellite marker를 이용한 불포화지방산 함량의 양적형질 유전자좌의 분석

  • Kim, Hyeun-Kyeung (Major of Horticulture Bioscience, Collage of Natural Resource & Life Science, Pusan National University) ;
  • Im, Moo-Hyeog (Dept. of Food Safety Evaluation, Korea Food & Drug Administration) ;
  • Choung, Myoung-Gun (Dept. of Herbal Medicine Resource, Kangwon National University)
  • 김현경 (부산대학교 생명자원개발연구소) ;
  • 임무혁 (식품의약품안전청 식품평가부) ;
  • 정명근 (강원대학교 생약자원개발학과)
  • Published : 2008.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Soybean oil is an important source of vegetable oil for human food and nonfood applications and accounts for approximately 22% of the world's total edible oil production. Improvement of the quality and quantity of soybean seed oil constituents is one of the most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control oleic, linoleic, and linolenic acid contents in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.85, 0.82 and 0.81, respectively. Eight independent QTLs for oleic acid content were identified from linkage group (LG) A2, C1, D2, F, G, L, and O. Seven QTLs for linoleic acid content were located on LG D1b, E, H, I and L. Oil content was related with five QTLs located on LG C1, H, J, K, and L. Oleic, linoleic, and linolenic acid have two common QTLs on LG C1 and L. Thus, we identified major loci improving soybean oil quality.

콩의 oil은 식량유지 자원으로서 매우 중요한 부분을 차지하고 있으며, 전세계 식용유의 22%를 콩 oil이 차지하고 있으며 식품에서 매우 중요한 영양학적인 요소이다. 이중 불포화지방산은 지방산 중에서 종자 구성물질들은 polygenetic 형질들로 되어있다. 본 시험은 큰올콩과${\times}$신팔달콩의 RIL 계통과 SSR marker를 이용하여 유전자지도를 작성하고, 이를 바탕으로 불포화지방산의 함량과 관련된 양적형질 유전자좌(QTLs)를 탐색하였다. Oleic acid 함량과 관련된 QTLs는 7개의 연관군에서 8개의 마커가 확인되었으며, linoleic acid는 5개의 연관군에서 7개의 마커가 확인되었다. 그리고 linolenic acid는 5개의 연관군에서 각각 하나씩의 마커가 확인되었다. 본 시험의 결과 불포화지방산에 공통적으로 나타난 QTL은 연관군 C1과 L이었다.

Keywords

References

  1. Brummer, E. C., A. D. Nickell, J. R. Wilcox and R. C. Shoemaker. 1995. Mapping the Fan locus controlling linolenic acid content in soybean oil. J. Hered. 86, 245-247 https://doi.org/10.1093/oxfordjournals.jhered.a111572
  2. Brummer, E. C., G. L. Graef, J. Orf, J. R. Wilcox and R. C. Shoemaker. 1997. Mapping QTL for seed protein and oil content eight soybean populations. Crop Sci. 37, 370-378 https://doi.org/10.2135/cropsci1997.0011183X003700020011x
  3. Diers, B. W. and R. C. Shoemaker. 1992. Restriction fragment length polymorphism analysis of soybean fatty acid content. J. Am. Oil. Chem. 69, 1242-1244 https://doi.org/10.1007/BF02637690
  4. Frey, K. J. and T. Horner. 1957 Heritability in standard units. Agron. J. 49, 59-62 https://doi.org/10.2134/agronj1957.00021962004900020001x
  5. Glaudements, H. D., M. M. J. Timmermans and H. Rijkse. 1998. The world of edible oils. pp. 1-5, Robobank International Marketing, Utrecht, the Netherlands
  6. Haldane, J. B. S. 1919. The combination of linkage values and the calculation of distances between the loci of linked factors. J. Genet. 8, 299-309
  7. Hu, F. B., M. J. Stampfer, J. E. Manson, E. Rimm, G. A. Colditz, B. A. Rosner, C. H. Hennekens and W. C. Willett. 1997. Dietary fat intake and the risk of coronary heart disease in women. N. Eng. J. Med. 337, 1491-1499 https://doi.org/10.1056/NEJM199711203372102
  8. Keirn, P., T. C. Olson and R. C. Shoemaker. 1988. A rapid protocol for isolating soybean DNA. Soybean Genet. Newsl. 15, 150-154
  9. Kim, H. K, S. T. Kang, M. G. Chung, C. S. Jung, K. W. Oh, I. Y. Baek and B. G. Son. 2006. Simple sequence repeat markers linked to quantitative trait loci controlling seed weight, protein and oil contents in soybean. J. Life Sci. 16, 949-954 https://doi.org/10.5352/JLS.2006.16.6.949
  10. Li, Z., R. F. Wilson, W. E. Rayford and H. R. Boerma. 2002. Molecular mapping genes conditioning reduced palmitic acid content. Crop Sci. 42, 373-378 https://doi.org/10.2135/cropsci2002.0373
  11. Mainly, K. F. and J. M. Olson. 1999. Overview of QTL mapping software and introduction to Map Manager QT. Mamm. Genome 10, 327-334 https://doi.org/10.1007/s003359900997
  12. Miller, J. F., D. C. Zimmerman and B. A. Vick. 1987. Genetic control of high oleic acid content in sunflower oil. Crop Sci. 27, 923-926 https://doi.org/10.2135/cropsci1987.0011183X002700050019x
  13. Maughan, P. J., M. A. Saghai, G. R. Buss. 2000. Identification of quantitative trait loci controlling sucrose content in soybean (Glycine max). Mol. Breed. 6, 105-111 https://doi.org/10.1023/A:1009628614988
  14. Rakow, G. and D. I. McGegor. 1973. Opportunities and problems in modification of levels of rapeseed C18 unsaturated fatty acid. J. Am. Oil Chem. Soc. 50, 400-403 https://doi.org/10.1007/BF02641817
  15. Robertson, J. A. and J. K. Tomas. 1976. Chemical and microbial changes in dehulled confectionary sunflower kernels during storage under controlled conditions. J. Milk Food Technol. 39, 18-23 https://doi.org/10.4315/0022-2747-39.1.18
  16. Song, Q. J., L. F. Marek, R. C. Shoemaker, K. G. Lark, V. C. Concibido, X. Delannay, J. E. Specht and P. B. Cregan. 2004. A new integrated genetic linkage map of the soybean. Theor. Appl. Genet. 109, 122-128 https://doi.org/10.1007/s00122-004-1602-3
  17. Tagagi, Y., S. M. Rahman and T. Anai. 1998. Construction of novel fatty acid composition in soybean oil by induced mutation. Gamma Field Symposia 37, 17-28
  18. Topfer, R, N. Martini and J. Schell. 1995. Modification of plant lipid synthesis. Science 268, 681-686 https://doi.org/10.1126/science.268.5211.681
  19. VIes, R. O. and J. J. Gottrnbos. 1989. Nutritional characteristics and food use of vegetable oils. In "Oil crops of the World, Their Breeding and Utilization" Robbelen, G. et al. (eds.), McGraw-Hill, New York. pp. 63-86
  20. Whittaker, J. C., R Thompson, D. M. Vissche. 1996. On the mapping of QTL by regression of phenotype on marker type. Heredity 77, 23-32 https://doi.org/10.1038/hdy.1996.104
  21. Wilson, R. F., J. W. Burton and C. A. Brim. 1981. Progress in the selection for altered fatty acid composition in soybean. Crop Sci. 27, 788-791