International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지

Effects of substance P on mineralization markers and heme oxygenase-1 Expression in human immortalized periodontal ligament cells

  • Cho, You-Min (Dept. of Orthodontics, College of Dentistry, Wonkwang University) ;
  • Suh, Chung-Hwan (Department of Oral & Maxillofacial Pathology, Wonkwang University) ;
  • Chun, Sang-Woo (Department of Oral & Maxillofacial Pathology, Wonkwang University) ;
  • Kim, Eun-Cheol (Department of Oral & Maxillofacial Pathology, Wonkwang University) ;
  • Kang, Kyung-Hwa (Department of Orthodontics, College of Dentistry, Wonkwang University)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Substance P (SP) is known to be expressed in the nerve fibers of dental pulp and periodontal tissues. It was recently reported that SP expression increased in response to orthodontic force. In the present study, we investigated the effect of SP on expression of mineralization markers and heme oxygenase-1 (HO-1) in human immortalized periodontal ligament (IPDL) cells. Cell viability was measured using a 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay. The expression of mineralization markers, including alkaline phosphatase (ALP), osteonectin (ON) and bone sialoprotein (BSP), and heme oxygenase-1 (HO-1) was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. SP did not significantly change human IPDL cell viability, with the exception of the 24 hour treatment group. Treatment of human IPDL cells with $10^{-10}$ to $10^{-4}M$ SP upregulated mineralization marker and HO-1 expression in a time- and concentration-dependent manner. Our results suggest that SP may modulate osteoblastic cell differentiation of human IPDL cells through a mechanism involving HO-1 expression.

Keywords

References

  1. Basdra EK and Domposch G. Osteoblast-like properties of human periodontal ligament cells : an in vitro analysis. Eur J Orthod. 1997;19:615-21 https://doi.org/10.1093/ejo/19.6.615
  2. Bjurholm A, Kreicberg A, Schulzberg M. Neuroendocrine regulation of cyclic AMP formation in osteoblastic cell lines (UMR-106-01, ROS 17/2.8, MC3T3-E1, ans Saos2) and primary bone cells. J Bone Miner Res. 1992;7:1011-9 https://doi.org/10.1002/jbmr.5650070903
  3. Cho MI, Matsuda N, Lin WL, Moshier A, Ramakrishnan PR. In vitro formation of mineralized nodules by periodontal ligament cells from the rat. Calcif Tissue Int. 1992;50:459-67 https://doi.org/10.1007/BF00296778
  4. Choi AM and Alam J. Heme oxygenase-1: function, regulation, and implication of a novel stress-inducible protein in oxidant-induced lung injury. Am J Respir Cell Mol Biol. 1996;15:9-19 https://doi.org/10.1165/ajrcmb.15.1.8679227
  5. Goto T and Tanaka T. Tachykinins and tachykinin receptors in bone. Microsc Res Tech. 2002;58:91-7 https://doi.org/10.1002/jemt.10123
  6. Hayashi K, Igarashi K, Miyoshi K, Shinoda H, Mitani H. Involvement of nitric oxide in orthodontic tooth movement in rats. Am J Orthod Dentofacial Orthop. 2002;122:306-9 https://doi.org/10.1067/mod.2002.126151
  7. Imai S and Matsusue Y. Neuronal regulation of bone metabolism and anabolism: calcitonin gene-related peptide-, substance P-, and tyrosine hydroxylase-containing nerves and the bone. Microsc Res Tech. 2002;58:61-9 https://doi.org/10.1002/jemt.10119
  8. Irie K, Hara-Irie F, Ozawa H, Yajima T. Calcitonin generelated peptide (CGRP)-containing nerve fibers in bone tissue and their involvement in bone remodeling. Microsc Res and Tech. 2002;58:85-90 https://doi.org/10.1002/jemt.10122
  9. Jeon IS. An immunohistochemical study on substance P and calcitonin gene-related peptide in the experimental tooth movement of rat. J Wonkwang Dent Res. 1997;7:63-84
  10. Kojima T, Yamaguchi M, Kasai K. Substance P stimulates release of RANKL via COX-2 expression in human dental pulp cells. Inflamm Res. 2006;55:78-84 https://doi.org/10.1007/s00011-005-0013-5
  11. Kvinnsland I and Heyeraas KJ. Effect of traumatic occlusion on CGRP and SP immunoreactive nerve fiber morphology in rat molar pulp and periodontium. Histochemistry. 1992;97:111-20 https://doi.org/10.1007/BF00267300
  12. Li MH, Cha YN, Surh YJ. Peroxynitrite induces HO-1 expression via PI3K/Akt-dependent activation of NF-E2-related factor 2 in PC12 cells. Free Radic Biol Med. 2006;41:1079-91 https://doi.org/10.1016/j.freeradbiomed.2006.06.010
  13. Long P, Liu F, Piesco NP, Kapur R, Agarwal S. Signaling by mechanical strain involves transcriptional regulation of proinflammatory genes in human periodontal ligament cells in vitro. Bone. 2002;30:547-52 https://doi.org/10.1016/S8756-3282(02)00673-7
  14. Lutherman J, Dahlof G, Modeer T, Johansson O. Immunohistochemical study of neuronal markers in human gingiva with phenytoin-induced overgrowth. Scand J Dent Res. 1988;96:339-46
  15. Matayoshi T, Goto T, Fukuhara E, Takano H, Kobayashi S, Takahashi T. Neuropeptide substance P stimulates the formation of osteoclasts via synovial fibroblastic cells. Biochem Biophys Res Commun. 2005;327:756-64 https://doi.org/10.1016/j.bbrc.2004.12.055
  16. Mizuno M, Imai T, Fujisawa R, Tani H, Kuboki Y. Bone sialoprotein (BSP) is a cucial factor for the expression of osteoblastic phenotypes of bone marrow cells cultured on type I collagen matrix. Calcif Tissue Int. 2000;66:388-96 https://doi.org/10.1007/s002230010078
  17. Mori T, Ogata T, Okumura H, Shibata T, Nakamura Y, Kataoka K. Substance P regulates the function of rabbit cultured osteoclast; increase of intracellular free calcium concentration and enhanced bone resorption. Biochem Biophys Res Commun. 1999;262:418-22 https://doi.org/10.1006/bbrc.1999.1220
  18. Nicolay OF, Davidovitch Z, Shanfeld JL, Alley K. Substance P immunoreactivity in periodontal tissues during orthodontic tooth movement. Bone Miner. 1990;11:19-29 https://doi.org/10.1016/0169-6009(90)90012-5
  19. Nohutcu RM, Mc Cauley LK, Koh AJ, Somerman MJ. Expression of extracellular matrix proteins in human periodontal ligament cells during mineralization in vitro. J Periodontol. 1997;68:320-7 https://doi.org/10.1902/jop.1997.68.4.320
  20. Nojima N, Kobayashi M, Shionome M, Takahashi N, Suda T, Hasegawa K. Fibroblastic cells derived from bovine periodontal ligaments have the phenotypes of osteoblasts. J Periodont Res. 1990;25:179-85 https://doi.org/10.1111/j.1600-0765.1990.tb01041.x
  21. Norevall LI, Matsson L, Forsgren S. Main sensory neuropeptides, but not VIP and NPY, are involved in bone remodeling during orthodontic tooth movement in the rat. Ann NY Acad Sci. 1998;865:353-9 https://doi.org/10.1111/j.1749-6632.1998.tb11195.x
  22. Norton LA, Andersen KL, Arenholt-Bindslev Dorthe, Andersen L, Melsen B. A methodical study of shape changes in human oral cells perturbed by a stimulated orthodontic strain in vitro. Arch Oral Biol. 1995;40:863-72 https://doi.org/10.1016/0003-9969(95)00044-P
  23. Oh JH and Choi BJ. Effects of substance P and VIP on the alkaline phosphatase activity and the gene expression of bone proteins in osteoblast-like cells. J Oral Biol. 1995;19:33-9
  24. Otterbein L, Sylvester SL, Choi AM. Hemoglobin provides protection against lethal endotoxemia in rats: the role of heme oxygenase-1. Am J Respir Cell Mol Biol. 1995;13:595-601 https://doi.org/10.1165/ajrcmb.13.5.7576696
  25. Pi SH, Lee SK, Hwang YS, Choi MG, Lee SK, Kim EC. Differential expression of periodontal ligament-specific markers and osteogenic differentiation in HPV16-immortalized human gingival fibroblasts and periodontal ligament cells. J Perio Res. 2007;42:104-13 https://doi.org/10.1111/j.1600-0765.2006.00921.x
  26. Sohn SJ. Substance P upregulates osteoclastogenesis by activating nuclear factor kappa B in osteoclast precursors. Acta Otolaryngol. 2005;125:130-3 https://doi.org/10.1080/00016480410017710
  27. Termine JD, Belcourt AB, Conn KM, Kleinman HK. Mineral and collagen binding proteins of fetal calf bone. J Biol Chem. 1981;256:10403-8
  28. Terry CM, Clickeman JA, Hoidal JR, Callahan KS. TNF-$\alpha$ and IL-1$\alpha$ induce heme oxygenase-1 via protein kinase C, $Ca^{2+}$ and phospholipase $A_2$ in endothelial cells. Am J Physiol. 1999;276:1493-501
  29. Vile GF, Basu-Modak S, Waltner C, Tyrrell RM. Heme oxygenase-1 mediates an adaptive response to oxidative stress in human skin fibroblasts. Proc Natl Acad Sci. 1994;91:2607-10 https://doi.org/10.1073/pnas.91.7.2607
  30. Zwerina J, Tzima S, Hayer S, Redlich K, Hoffmann O, Hanslik-Schnabel B, Smolen JS, Kollias G, Schett G. Heme oxygenase 1 (HO-1) regulates osteoclastogenesis and bone resorption. FASEB J. 2005;19:2011-3 https://doi.org/10.1096/fj.05-4278fje