Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Effect of Methylprednisolone Sodium Succinate on Innate Immune Function of Canine Peripheral Blood Phagocytes

  • Park, Moo-Rim (College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Ji-Houn (College of Veterinary Medicine, Chungbuk National University) ;
  • Yang, Mhan-Pyo (College of Veterinary Medicine, Chungbuk National University)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Glucocorticoids (GCs) are the most widely used immunosuppressive agents, but animals treated with GCs may experience deleterious side effects which limit their use in many clinical conditions. In the present study, we examined whether methylprednisolone sodium succinate (MPSS), a glucocorticoid, modulates circulating leukocyte numbers, phagocytic capacity and oxidative burst activity (OBA) of canine peripheral blood phagocytes, and whether tumor necrosis factor-alpha (TNF-$\alpha$) release is affected by MPSS injection. Neutrophilia and monocytosis were induced by the administration of a high dose of MPSS, which is the recommended protocol for canine patients with acute spinal cord injury. The injection of MPSS decreased the phagocytic capacity of canine PMNs but not PBMCs, and recovered 12 hours (hr) after the completion of MPSS dosing. The OBA of both PMNs and PBMCs was suppressed by MPSS, and restored 24 hr after the completion of dosing. The lipopolysaccharide-induced TNF-α release by PBMCs but not PMNs exposed to MPSS was reduced 12 hr after the completion of dosing, and recovered 48 hr after the completion of dosing. These results suggest that the application of MPSS protocol inhibits the innate immune functions of canine peripheral blood phagocytes for short time relatively.

Keywords

References

  1. Aderem A. Role of Toll-like receptors in inflammatory response in marcrophages. Crit Care Med 2001; 29: 16-18
  2. Babior BM. Oxidants from phagocytes: agents of defense and destruction. Blood 1984; 64: 959-966
  3. Baccan GC, Oliveira RD, Mantovani B. Stress and immunological phagocytosis: possible nongenomic action of corticosterone. Life Sci 2004; 75: 1357-1368 https://doi.org/10.1016/j.lfs.2004.02.026
  4. Boyaka PN, McGhee JR. Cytokines as adjuvants for the induction of mucosal immunity. Adv Drug Deliv Rev 2001; 51: 71-79 https://doi.org/10.1016/S0169-409X(01)00170-3
  5. Bracken MB, Holford TR. Effects of timing of methylprednisolone or naloxone administration on recovery of segmental and long-tract neurological function in NASCIC 2. J Neurosurg 1993; 79: 500-507
  6. Braughler JM, Hall ED, Means ED, Waters TR, Anderson DK. Evaluation of an intensive methylprednisolone sodium succinate dosing regimen in experimental spinal cord injury. J Neurosurg 1987; 67: 102-105 https://doi.org/10.3171/jns.1987.67.1.0102
  7. Brown SA, Hall ED. Role of oxygen-derived free radicals in the pathogenesis of shock and trauma, with focus on central nervous system injuries. J Am Vet Med Assoc 1992; 200: 1849-1859
  8. Chen YZ, Hua SY. Effects of glucocorticoid on electrical activities of mammalian sympathetic ganglion cells in vitro. Neurosci Suppl 1987; 22: 1008-1013
  9. Dahlgren C, Karlsson A. Respiratory burst in human neutrophils. J Immunol Methods 1999; 232: 3-14 https://doi.org/10.1016/S0022-1759(99)00146-5
  10. Duval D, Durant S, Homo-Delarche F. Non-genomic effects of steroids. Interactions of steroid molecules with membrane structures and functions. Biochim Biophys Acta 1983; 737: 409-442 https://doi.org/10.1016/0304-4157(83)90008-4
  11. Elbim C, Chollet-Martin S, Bailly S, Hakim J, Gougerot- Pocidalo MA. Priming of polymorphonuclear neutrophils by tumor necrosis factor alpha in whole blood: identification of two polymorphonuclear neutrophil subpopulation in response to formyl-peptides. Blood 1993; 82: 633-640
  12. Fessler MB, Arndt PG, Just I, Nick JA, Malcolm KC, Worthen GS. Dual role for RhoA in suppression and induction of cytokines in the human neutrophil. Blood 2007; 109: 1248-1256
  13. Gerndt SJ, Rodriguez JL, Pawlik JW, Taheri PA, Wahl WL, Micheal AJ, Papadopoulos SM. Consequences of high-dose steroid therapy for acute spinal cord injury. J Trauma 1997; 42: 279-284 https://doi.org/10.1097/00005373-199702000-00017
  14. Haar D, Nielsen H. The effects of betamethasone, methylprednisolone and hydrocortisone on lucigenin- and luminol-amplified chemiluminescence generated in human monocytes. Scand J Rheumatol 1988; 17: 487-490 https://doi.org/10.3109/03009748809098812
  15. Hall ED. The neuroprotective pharmacology of methylprednisolone. J Neurosurg 1992; 76: 13-22 https://doi.org/10.3171/jns.1992.76.1.0013
  16. Hampton MB, Kettle AJ, Winterbourn CC. Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing. Blood 1998; 92: 3007-3017
  17. Han J, Beutler B. The essential role of the UA-rich sequence in endotoxin-induced cachectin/TNF synthesis. Eur Cytokine Netw 1990; 1: 71-75
  18. Haugen SE, Wiik P. Glucocorticoid and ACTH regulation of rat peritoneal phagocyte chemiluminescence and nitric oxide production in culture. Acta Physiol Scand 1997; 161: 93-101 https://doi.org/10.1046/j.1365-201X.1997.00179.x
  19. Hurlbert RJ. Methylprednisolone for acute spinal cord injury: an inappropriate standard of care. J Neurosurg 2000; 93: 1-7 https://doi.org/10.3171/jns.2000.93.1.0001
  20. Kang JH, Yang MP. In vitro evaluation of the effect of trans-10, cis-12 conjugated linoleic acid on phagocytosis by canine peripheral blood polymorphonuclear neutrophilic leukocytes exposed to methylprednisolone sodium succinate. Am J Vet Res 2008; 69: 494-500 https://doi.org/10.2460/ajvr.69.4.494
  21. Kobayashi T, Robinson JM, Seguchi H. Identification of intracellular sites of superoxide production in stimulated neutrophils. J Cell Sci 1998; 111: 81-91
  22. Latimer KS, Rakich PM. Clinical interpretation of leukocyte responses. Vet Clin North Am Small Anim Pract 1989; 19: 637-668 https://doi.org/10.1016/S0195-5616(89)50077-9
  23. Lee WL, Harrison RE, Grinstein S. Phagocytosis by neutrophils. Microbes Infect 2003; 5: 1299-1306 https://doi.org/10.1016/j.micinf.2003.09.014
  24. Liles WC, Dale DC, Klebanoff SJ. Glucocorticoids inhibit apoptosis of human neutrophils. Blood 1995; 86: 3181-3188
  25. Long F, Wang YX, Liu L, Zhou J, Cui RY, Jiang CL. Rapid nongenomic inhibitory effects of glucocorticoids on phagocytosis and superoxide anion production by macrophages. Steroids 2005; 70: 55-61 https://doi.org/10.1016/j.steroids.2004.10.004
  26. Lowenberg M, Tuynman J, Bilderbeek J, Gaber T, Buttgereit F, van Deventer S, Peppelenbosch M, Hommes D. Rapid immunosuppressive effects of glucocorticoids mediated through Lck and Fyn. Blood 2005; 106: 1703-1710 https://doi.org/10.1182/blood-2004-12-4790
  27. McEvoy GK. Adrenals. In : American Hospitals Formulary Service Drug Information 91. Bethesda, Maryland: American Society of Hospital Pharmacists, Inc. 1991:1810-1830
  28. Molina JM. Garrido A, Anguiano A, Uceda A, Serrano E. Experimental study of the effect of 6-methyl-prednisolone on ingestion-microbicidal function activity in dog neutrophils. Comp Immunol Microbiol Infect Dis 1991; 14: 21-29 https://doi.org/10.1016/0147-9571(91)90037-E
  29. Muller S, Masson V, Droesch S, Donner M, Stoltz JF. Phagocytosis and membrane fluidity: application to the evaluation of opsonizing properties of fibronectin. Biorheology 1989; 26: 323-330 https://doi.org/10.3233/BIR-1989-26216
  30. Nathan CF. Neutrophil activation on biological surfaces. Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes. J Clin Invest 1987; 80: 1550-1560 https://doi.org/10.1172/JCI113241
  31. Nelson DH, Meikle AW, Benowitz B, Murray DK, Ruhmann-Wennhold A. Cortisol and dexamethasone suppression of superoxide anion production by leukocytes from normal subjects. Trans Assoc Am Physicians 1978; 91: 381-387
  32. Richardson MP, Ayliffe MJ, Helbert M, Davies EG. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods 1998; 219: 187-193 https://doi.org/10.1016/S0022-1759(98)00136-7
  33. Roshol H, Skrede KK, AEro CE, Wiik P. Dexamethasone and methylprednisolone affect rat peritoneal phagocyte chemiluminescence after administration in vivo. Eur J Phamacol 1995; 286: 9-17 https://doi.org/10.1016/0014-2999(95)00430-S
  34. Segal AW, Wientjes F, Stockley RW, Dekker LV. Components and organization of the NADPH oxidase of phagocytic cells. In: Phagocytosis: the host. Stamford: JAI press Inc, 1999; 1: 441-483 https://doi.org/10.1016/S1874-5172(99)80043-1
  35. Smith GS. Neutrophils. In: Schalm's veterinary hematology. 5th ed. Philadelphia: Lippincott Williams & Wilkins. 2000; 281-296
  36. Steer JH, Kroeger KM, Abraham LJ, Joyce DA. Glucocorticoids suppress tumor necrosis factor-alpha expression by human monocytic THP-1 cells by suppressing transactivation through adjacent NF-kappa B and c-Junactivating transcription factor-2 binding sites in the promoter. J Biol Chem 2000; 275: 18432-18440 https://doi.org/10.1074/jbc.M906304199
  37. Steer JH, Ma DT, Dusci L, Garas G, Pederson KE, Joyce DA. Altered leukocyte trafficking and suppressed tumor necrosis factor alpha release from peripheral blood monocytes after intra-articular glucocorticoid treatment. Ann Rheum Dis 1998; 57: 732-737 https://doi.org/10.1136/ard.57.12.732
  38. Steer JH, Vuong Q, Joyce DA. Suppression of human monocyte tumor necrosis factor-alpha release by glucocorticoid therapy: relationship to systemic monocytopaenia and cortisol suppression. Br J Clin Phamacol 1997; 43: 383-389 https://doi.org/10.1111/j.1365-2125.1997.00586.x
  39. Thomas WB. Disorders of the spinal cord. In: Handbook of small animal practice. 4th ed. Philadelphia: Saunders. 2003; 256-275
  40. Wehling M. Specific, nongenomic actions of steroid hormones. Annu Rev Physiol 1997; 59: 365-93 https://doi.org/10.1146/annurev.physiol.59.1.365