Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Albumin and Antioxidants Inhibit Serum-deprivation-induced Cell Adhesion in Hematopoietic Cells

  • Han, Mi-Jin (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University) ;
  • Lim, Sung-Mee (Department of Food Science & Technology, Tongmyong University) ;
  • Kim, Yu-Lee (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University) ;
  • Kim, Hyo-Lim (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University) ;
  • Kim, Kye-Ok (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University) ;
  • Sacket, Santosh J. (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University) ;
  • Jo, Ji-Yeong (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University) ;
  • Bae, Yoe-Sik (Department of Biochemistry, College of Medicine, Dong-A University) ;
  • Okajima, Fumikazu (Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University) ;
  • Im, Dong-Soon (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute for Drug Development, Pusan National University)
  • Published : 2008.12.31
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Abstract

Previously, we identified albumin as an inhibitory factor in serum for cell adhesion of T cells such as human Jurkat T and primary cultured human T cells. In the present study, we found that other hematopoietic cell lines including U-937 human monocytes, THP-1 human monocytes, K-562 promyelocytic leukemia cells, and HL-60 human leukemia cells, also adhere to tissue culture flasks when serum is withdrawn, and albumin exerts an inhibitory effect on cell adhesion by those cells, implying that this inhibition is a common phenomenon in hematopoietic cells. Furthermore, we found that cell adhesion is inhibited by antioxidants such as (-)-epigallocatechin- 3-gallate (EGCG), morin, and a-tocopherol. Our results suggest that albumin may inhibit basal cell adhesion of hematopoietic cells and that the oxidative balance in the plasma may be important for cell adhesion of hematopoietic cells in vivo.

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References

  1. Azzi, A. (2007). Molecular mechanism of alpha-tocopherol action. Free Radic. Biol. Med. 43, 16-21 https://doi.org/10.1016/j.freeradbiomed.2007.03.013
  2. Campanero, M. R., Sanchez-Mateos, P., del Pozo, M. A. and Sanchez-Madrid, F. (1994). ICAM-3 regulates lymphocyte morphology and integrin-mediated T cell interaction with endothelial cell and extracellular matrix ligands. J. Cell Biol. 127, 867-878 https://doi.org/10.1083/jcb.127.3.867
  3. Chen, Y. H., Lin, S. J., Chen, Y. L., Liu, P. L. and Chen, J. W. (2006). Anti-inflammatory effects of different drugs/agents with antioxidant property on endothelial expression of adhesion molecules. Cardiovasc. Hematol. Disord. Drug Targets 6, 279-304 https://doi.org/10.2174/187152906779010737
  4. Collins, T. L., Kassner, P. D., Bierer, B. E. and Burakoff, S. J. (1994). Adhesion receptors in lymphocyte activation. Curr. Opin. Immunol. 6, 385-393 https://doi.org/10.1016/0952-7915(94)90117-1
  5. Dailey, M. O. (1998). Expression of T lymphocyte adhesion molecules: regulation during antigen-induced T cell activation and differentiation. Crit. Rev. Immunol. 18, 153-184 https://doi.org/10.1615/CritRevImmunol.v18.i3.10
  6. Dustin, M. L. and de Fougerolles, A. R. (2001). Reprogramming T cells: the role of extracellular matrix in coordination of T cell activation and migration. Curr. Opin. Immunol. 13, 286-290 https://doi.org/10.1016/S0952-7915(00)00217-X
  7. Faruqi, R., de la Motte, C. and DiCorleto, P. E. (1994). Alphatocopherol inhibits agonist-induced monocytic cell adhesion to cultured human endothelial cells. J. Clin. Invest. 94, 592-600 https://doi.org/10.1172/JCI117374
  8. Hung, C. F., Huang, T. F., Chiang, H. S. and Wu, W. B. (2005). (-)-Epigallocatechin-3-gallate, a polyphenolic compound from green tea, inhibits fibroblast adhesion and migration through multiple mechanisms. J. Cell Biochem. 96, 183-197 https://doi.org/10.1002/jcb.20509
  9. Kawai, K., Tsuno, N. H., Kitayama, J., Okaji, Y., Yazawa, K., Asakage, M., Hori, N., Watanabe, T., Takahashi, K. and Nagawa, H. (2004). Epigallocatechin gallate attenuates adhesion and migration of CD8+ T cells by binding to CD11b. J. Allergy Clin. Immunol. 113, 1211-1217 https://doi.org/10.1016/j.jaci.2004.02.044
  10. Kim, Y. L., Im, Y. J., Lee, Y. K., Ha, N. C., Bae, Y. S., Lim, S. M., Okajima, F. and Im, D. S. (2006). Albumin functions as an inhibitor of T cell adhesion in vitro. Biochem. Biophys. Res. Commun. 351, 953-957 https://doi.org/10.1016/j.bbrc.2006.10.143
  11. Kobuchi, H., Roy, S., Sen, C. K., Nguyen, H. G. and Packer, L. (1999). Quercetin inhibits inducible ICAM-1 expression in human endothelial cells through the JNK pathway. Am. J. Physiol. 277, C403-411 https://doi.org/10.1152/ajpcell.1999.277.3.C403
  12. Ludwig, A., Lorenz, M., Grimbo, N., Steinle, F., Meiners, S., Bartsch, C., Stangl, K., Baumann, G. and Stangl, V. (2004). The tea flavonoid epigallocatechin-3-gallate reduces cytokineinduced VCAM-1 expression and monocyte adhesion to endothelial cells. Biochem. Biophys. Res. Commun. 316, 659-665 https://doi.org/10.1016/j.bbrc.2004.02.099
  13. Manna, S. K., Aggarwal, R. S., Sethi, G., Aggarwal, B. B. and Ramesh, G. T. (2007). Morin (3,5,7,2',4'-Pentahydroxyflavone) abolishes nuclear factor-kappaB activation induced by various carcinogens and inflammatory stimuli, leading to suppression of nuclear factor-kappaB-regulated gene expression and up-regulation of apoptosis. Clin. Cancer Res. 13, 2290-2297 https://doi.org/10.1158/1078-0432.CCR-06-2394
  14. Quinlan, G. J., Martin, G. S. and Evans, T. W. (2005). Albumin: biochemical properties and therapeutic potential. Hepatology 41, 1211-1219 https://doi.org/10.1002/hep.20720
  15. Roy, S., Sen, C. K. and Packer, L. (1999). Determination of cellcell adhesion in response to oxidants and antioxidants. Methods Enzymol. 300, 395-401 https://doi.org/10.1016/S0076-6879(99)00144-5
  16. Sazuka, M., Isemura, M. and Isemura, S. (1998). Interaction between the carboxyl-terminal heparin-binding domain of fibronectin and (-)-epigallocatechin gallate. Biosci. Biotechnol. Biochem. 62, 1031-1032 https://doi.org/10.1271/bbb.62.1031
  17. Sellak, H., Franzini, E., Hakim, J. and Pasquier, C. (1994). Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation. Blood 83, 2669-2677
  18. Sen, C. K. and Roy, S. (2001). Antioxidant regulation of cell adhesion. Med. Sci. Sports Exerc. 33, 377-381 https://doi.org/10.1097/00005768-200103000-00007
  19. Suzuki, Y. and Isemura, M. (2001). Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to laminin. Cancer Lett. 173, 15-20 https://doi.org/10.1016/S0304-3835(01)00685-1
  20. Traber, M. G. and Atkinson, J. (2007). Vitamin E, antioxidant and nothing more. Free Radic. Biol. Med. 43, 4-15 https://doi.org/10.1016/j.freeradbiomed.2007.03.024
  21. Tseng, S. Y. and Dustin, M. L. (2002). T-cell activation: a multidimensional signaling network. Curr. Opin. Cell Biol. 14, 575-580 https://doi.org/10.1016/S0955-0674(02)00370-8
  22. Zhang, G., Miura, Y. and Yagasaki, K. (2000). Suppression of adhesion and invasion of hepatoma cells in culture by tea compounds through antioxidative activity. Cancer Lett. 159, 169-173 https://doi.org/10.1016/S0304-3835(00)00545-0

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