Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Induction of Cardiovascular Anaphylaxis and Basic Pharmacological Analysis of Involved Mediators in Pithed Rats

  • Park, Kwan-Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Active cardiovascular anaphylactic response was induced in ovalbumin-sensitized, pithed Sprague-Dawley and Wistar rats. On intravenous administration of the antigen, ovalbumin, marked tachycardia and pressor responses were immediately elicited. Thereafter, a delayed long-lasting severe hypotensive response was observed. These anaphylactic cardiovascular responses were maximal 2-3 weeks after the sensitization, and the response was slightly diminished 6 weeks after sensitization. The immediate pressor response was blocked by a non-selective serotonin antagonist methysergide at a dose-dependent manner, but not by histamine receptor antagonists mepyramine (pyrilamine) or cimetidine. The delayed hypotension was reduced either by histamine $H_1$ receptor antagonist mepyramine or $H_2$ receptor antagonist cimetidine, both in a dose-dependent manner. The tachycardic response was not influenced by serotonin or histamine receptor antagonists examined in this study. Differently from the cardiovascular responses, there was no observable bronchial contraction in Sprague-Dawley rat trachea in contrast to Wistar rat where the trachea contracted to in vitro antigen challenge. The cardiovascular anaphylactic model seems to be useful for studying cardiovascular events that occur exclusively in peripheral heart-blood vessel systems. The involvement of two major anaphylactic mediators, serotonin and histamine, is partially demonstrated.

Keywords

References

  1. Bartlett, L. A. (1963). The action of histamine on the isolated heart. Brit. J. Pharmacol. Chemother. 21, 450-461 https://doi.org/10.1111/j.1476-5381.1963.tb02013.x
  2. Church, M. K. (1975). Response of rat lung to humoral mediators of anaphylaxis and its modification by drugs and sensitization. Br. J. Pharmacol. 55, 423-430 https://doi.org/10.1111/j.1476-5381.1975.tb06947.x
  3. El-Shanawany T., Williams P. E. and Jolles S. (2008). Clinical immunology review series: an approach to the patient with anaphylaxis. Clin. Exp. Immunol. 153, 1-9 https://doi.org/10.1111/j.1365-2249.2008.03694.x
  4. Farmer J. B., Richards, I. M. Sheard, P. and Woods A. M. (1975). Mediators of passive lung anaphylaxis in the rat. Brit. J. Pharmacol. 55, 57-64 https://doi.org/10.1111/j.1476-5381.1975.tb07610.x
  5. Frodi, G., Montopoli, M., Zanetti, M, Dorigo, P. and Caparrotta, L. (2008). $5-HT_{1B}$ receptor subtype and aging rat blood vessels. Pharmacology 81, 70-78 https://doi.org/10.1159/000109297
  6. Gillespie, J. S., MaClaren, A. and Pollock, D. (1970). A method of stimulating different segments of the autonomic outflow from the spinal column to various organs in the pithed cat and rat. Br. J. Pharmacol. 40, 257-267 https://doi.org/10.1111/j.1476-5381.1970.tb09919.x
  7. Gomes, J. C., Di Stasi, L. C., Sgarbosa, F. and Barata, L. E. S. (1994). Pharmacological evaluation of the inhibitory effect of extracts from Anchietia salutaris on the histamine release induced in the rat and the guinea pig. Int. Arch. Allergy Immunol. 103, 188-193 https://doi.org/10.1159/000236626
  8. Grave, L. M., Robertson, D. A., Levi, R., Beaker, C. G., Weksler, B. B., and Gay, W. A. (1996). IgE-mediated hypersensitivity in human heart tissue: histamine release and functional changes. J. Allergy Clin. Immunol. 77, 709-714 https://doi.org/10.1016/0091-6749(86)90415-X
  9. Kamei, C., Sugimoto, Y., Nakamura, S. and Zhong, C. (1995). Effect of (Z)-11-[3-(dimethylamino)propyridene]-6,11-dihydrodibenz[b,e]oxepin-2-acetic acid hydrochloride on experimental allergic conjunctivitis and rhinitis in rats and guinea pigs. Arzneim.-Forsch. 45, 1105-1108
  10. Katayama, S., Shionoya, H. and Ohtake, S. (1978). A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous anaphylaxis in guinea pigs and rats. Microbiol. Immunol. 22, 89-101 https://doi.org/10.1111/j.1348-0421.1978.tb00352.x
  11. Kelly, J. F. and Patterson, R. (1974). Anaphylaxis: course, mechanisms and treatment. JAMA 227, 1431-1436
  12. Lecomte, J. and Cession-Fossion, A. (1970). Absence de reactions, anaphylactiques localisees au mycarde du rat. C. R. Soc. Biol. (Paris) 164, 1420-1422
  13. Lei, H. Y., Chen, H. I., Chan, S. H., Lei., S. S., Lim, S. B. and Wing, L. Y. (1992). Antigen-specific tachycardia and hypertension in rodents. Clin. Exp. Allergy 22. 767-773 https://doi.org/10.1111/j.1365-2222.1992.tb02817.x
  14. Levi, R. (1988). Cardiac anaphylaxis: models, mediators, mechanisms, and clinical considerations. In: Human Inflammatory Disease, Cliniacl Immunology Vol. 1, (G, Marone, L. M Lichtenstein, M. Condorelli and A. S. Fauci Eds.), pp Decker, Toronto, Canada
  15. Lima, A. O. (1967). Pharmacologically active substances released during anaphylactic shock in the mouse. Int. Arch. Allergy 32, 46-54 https://doi.org/10.1159/000229915
  16. Malinnowska, B. and Schlicker, E. (1993). Identification of vascular endothelial $H_14$, $H_2$ and cardiac presynaptic $H_34 receptors in the pithed rats. Naunyn-Schmiedeberg's Arch. Pharmacol. 343, 55-60
  17. Misawa, M. (1988). A new rhinitis model using chemical mediators in rats. Jpn. J. Pharmacol. 48, 145-22 https://doi.org/10.1254/jjp.48.15
  18. Park, K. H., Park, J., Koh, D., and Lim, Y. (2002). Effect of sakosaponin-A, a terpenoid glycoside, isolated from Bulpreum falcatum on experimental allergic asthma. Phytother. Res. 16, 359-363 https://doi.org/10.1002/ptr.903
  19. Shipley, R. E. and Tilden, J. H. (1947). A pithed rat preparation suitable for assaying pressor substances. Proc. Soc. Exp. Biol. Med. 64, 453-455 https://doi.org/10.3181/00379727-64-15828
  20. Weil, M. H., Tang, W., Gazmuri, R. J., Bisera, J., Greenberg, S. R. and Kim, Y. B. (1992). Cardiac anaphylaxis in the Sprague-Dawley rats. J. Lab. Clin. Med. 120, 589-596
  21. Saito, H. and Nomura, Y. (1989). Screening methods for drug evaluation III, In: Pharmaceutical Research and Development, Vol. 9, eds. by (I. Suzuki, Ed.), pp Hirokawa Publishing Co., Tokyo, Japan
  22. Vleeming, W., Van Rooij, H. H., Wemer, J. and Porsius, A. J. (1991). Characterization and modulation of antigen-induced effects in isolated rat heart. J. Cardiovasc. Pharmacol. 18, 556-565 https://doi.org/10.1097/00005344-199110000-00012

Cited by

  1. Effects of White Mulberry (Morus alba) Leaves on Blood Vessel Reactivity in Hyperchloesterolemic Rats vol.28, pp.3, 2013, https://doi.org/10.13103/JFHS.2013.28.3.195