Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antiinflammatory Effect of Lactic Acid Bacteria: Inhibition of Cyclooxygenase-2 by Suppressing Nuclear Factor-${\kappa}B$ in Raw264.7 Macrophage Cells

  • Lee, Jeong-Min (Department of Medical Nutrition, Graduate School of East-West Medical Science and Research Institute of Clinical Nutrition, Kyung Hee University) ;
  • Hwang, Kwon-Tack (Department of Food and Nutrition, Chonnam National University) ;
  • Jun, Woo-Jin (Department of Food and Nutrition, Nambu University) ;
  • Park, Chang-Soo (Department of Pathology, Chonnam National University Medical School) ;
  • Lee, Myung-Yul (Department of Food and Nutrition, Chosun University)
  • Published : 2008.10.31
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Abstract

Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited the secretion of tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and prostaglandins $E_{2}\;(PGE_{2})$, followed by suppression of COX-2. To clarify the molecular mechanism, the inhibitory effect of L. casei 3260 on the NF-${\kappa}B$ signaling pathway was examined based on the luciferase reporter activity. Although the treatment of Raw264.7 cells with L. casei 3260 did not affect the transcriptional activity of NF-${\kappa}B$, it did inhibit NF-${\kappa}B$ activation, as determined by the cytosolic p65 release and degradation of I-${\kappa}B{\alpha}$. Therefore, these findings suggest that the suppression of COX-2 through inhibiting the NF-${\kappa}B$ activation by LPS may be associated with the antiinflammatory effects of L. casei 3260 on Raw264.7 cells.

Keywords

References

  1. Avonts, L., E. V. Uytven, and L. D. Vuyst. 2004. Cell growth and bacteriocin production of probiotic Lactobacillus strains in different media. Int. Dairy J. 14: 947-955 https://doi.org/10.1016/j.idairyj.2004.04.003
  2. Bai, S. K., S. J. Lee, H. J. Na, K. S. Ha, J. A. Han, H. Lee, Y. G. Kwon, C. K. Chung, and Y. M. Kim. 2005. ${\beta}-Carotene$ inhibits inflammatory gene expression in LPS-stimulated macrophage by suppressing redox-based $NF-{\kappa}B$ activation. Exp. Mol. Med. 37: 323-334 https://doi.org/10.1038/emm.2005.42
  3. Bautista-Garfias, C. R., O. Ixta, M. Orduna, F. Martinez, B. Aguilar, and A. Cortes. 1999. Enhancement of resistance in mice treated with Lactobacillus casei: Effect on Trichinella spiralis infection. Vet. Parasitol. 80: 251-260 https://doi.org/10.1016/S0304-4017(98)00210-6
  4. Billich, A., F. Bornancin, D. Mechtcheriakova, F. Natt, D. Huesken, and T. Baumruker. 2005. Basal and induced sphingosine kinase 1 activity in A549 carcinoma cells: Function in cell survival and IL-1 beta and TNF-alpha induced production of inflammatory mediators. Cell Signal. 17: 1203-1217 https://doi.org/10.1016/j.cellsig.2004.12.005
  5. Ching, L. S. and S. Mohamed. 2001. ${\alpha}-Tocopherol$ content in 62 edible tropical plants. J. Agric. Food Chem. 49: 3101-3105 https://doi.org/10.1021/jf000891u
  6. Dewitt, D. L. and E. A. Meade. 1993. Serum and glucocorticoid regulation of gene transcription and expression of prostaglandin H synthase-1 and prostaglandin H synthase-2 isozymes. Arch. Biochem. Biophys. 306: 94-102 https://doi.org/10.1006/abbi.1993.1485
  7. Dixon, D. A., C. D. Kaplan, T. M. McIntyre, G. A. Zimmerman, and S. M. Prescott. 2000. Post-transcriptional control of cyclooxygenase-2 gene expression. J. Biol. Chem. 275: 11750-11757 https://doi.org/10.1074/jbc.275.16.11750
  8. Ghosh, S., M. J. May, and E. B. Kopp. 1998. $NF-{\kappa}B$ and Rel proteins: Evolutionarily conserved mediators of immune responses. Annu. Rev. Immunol.16: 225-260 https://doi.org/10.1146/annurev.immunol.16.1.225
  9. Gilmore, T. D. 1999. The Rel/$NF-{\kappa}B$ signal transduction pathway: Introduction. Oncogene 18: 6842-6844 https://doi.org/10.1038/sj.onc.1203237
  10. Huang, W. C., J. J. Chen, and C. C. Chen. 2003. c-Src-dependent tyrosine phosphorylation of IKKbeta is involved in tumor necrosis factor-alpha-induced intercellular adhesion molecule-1 expression. J. Biol. Chem. 278: 9944-9952 https://doi.org/10.1074/jbc.M208521200
  11. Johnson-Henry, K. C., D. J. Mitchell, Y. Avitzur, E. Galindo-Mata, N. L. Jones, and P. M. Sherman. 2004. Probiotics reduce bacterial colonization and gastric inflammation in H. pylori-infected mice. Dig. Dis. Sci. 49: 1095-1102 https://doi.org/10.1023/B:DDAS.0000037794.02040.c2
  12. Kabe, Y., K. Ando, S. Hirao, M. Yoshida, and H. Handa. 2005. Redox regulation of $NF-{\kappa}B$ activation: Distinct redox regulation between the cytoplasm and the nucleus. Antioxid. Redox Signal. 7: 395-403 https://doi.org/10.1089/ars.2005.7.395
  13. Karin, M. and Y. Ben-Neriah. 2000. Phosphorylation meets ubiquitination: The control of $NF-{\kappa}B$ activity. Annu. Rev. Immunol. 18: 621-663 https://doi.org/10.1146/annurev.immunol.18.1.621
  14. Lee, J., K. T. Hwang, M. S. Heo, J. H. Lee, and K. Y. Park. 2005. Resistance of Lactobacillus plantarum KCTC 3099 from kimchi to oxidative stress. J. Med. Food 8: 299-304 https://doi.org/10.1089/jmf.2005.8.299
  15. Lee, J., N. Kim, D. H. Cho, M. Y. Chung, K. T. Hwang, H. J. Kim, W. Jun, and C. S. Park. 2006. Ethanol extract of Oenanthe javanica modulates inflammatory response by inhibiting $NF-{\kappa}B$ mediated COX-2 expression in Raw264.7 macrophage. Food Sci. Biotechnol. 15: 303-307
  16. Lim, J. W., H. Kim, and K. H. Kim. 2001. Nuclear factor-kappaB regulates cyclooxygenase-2 expression and cell proliferation in human gastric cancer cells. Lab. Invest. 81: 349-360 https://doi.org/10.1038/labinvest.3780243
  17. Menard, S., C. Candalh, J. C. Bambou, K. Terpend, N. Cerf-Bensussan, and M. Heyman. 2004. Lactic acid bacteria secrete metabolites retaining antiinflammatory properties after intestinal transport. Gut 53: 821-828 https://doi.org/10.1136/gut.2003.026252
  18. Newton, R., L. M. Kuitert, M. Bergmann, I. M. Adcock, and P. J. Barnes. 1997. Evidence for involvement of NF-kappaB in the transcriptional control of COX-2 gene expression by IL-1beta. Biochem. Biophys. Res. Commun. 237: 28-32 https://doi.org/10.1006/bbrc.1997.7064
  19. Pena, J. A. and J. Versalovic. 2003. Lactobacillus rhamnosus GG decreases TNF-alpha production in lipopolysaccharide-activated murine macrophages by a contact-independent mechanism. Cell Microbiol. 5: 277-285 https://doi.org/10.1046/j.1462-5822.2003.t01-1-00275.x
  20. Riedel, C. U., F. Foata, D. Philippe, O. Adolfsson, B. J. Eikmanns, and S. Blum. 2006. Antiinflammatory effects of bifidobacteria by inhibition of LPS-induced $NF-{\kappa}B$ activation. World J. Gastroenterol. 12: 3729-3735 https://doi.org/10.3748/wjg.v12.i23.3729
  21. Rosen, G. D., T. M. Birkenmeier, A. Raz, and M. J. Holtzman. 1989. Identification of a cyclooxygenase-related gene and its potential role in prostaglandin formation. Biochem. Biophys. Res. Commun. 164: 1358-1365 https://doi.org/10.1016/0006-291X(89)91819-6
  22. Shimizu, M., A. Deguchi, A. K. Joe, J. F. Mckoy, H. Moriwaki, and I. B. Weinstein. 2005. EGCG inhibits activation of HER3 and expression of COX-2 in human colon cancer cells. J. Exp. Ther. Oncol. 5: 69-78
  23. Smith, W. L., D. L. Dewitt, and R. M. Garavito. 2000. Cyclooxygenase: Structural, cellular, and molecular biology. Annu. Rev. Biochem. 69: 145-182 https://doi.org/10.1146/annurev.biochem.69.1.145
  24. Trushin, S. A., K. N. Pennington, E. M. Carmona, S. Asin, D. N. Savoy, D. D. Billadeau, and C. V. Paya. 2003. Protein kinase Calpha (PKCalpha) acts upstream of PKtheta to activate I$\kappa$B kinase and $NF-{\kappa}B$ in T lymphocytes. Mol. Cell Biol. 23: 7068-7081 https://doi.org/10.1128/MCB.23.19.7068-7081.2003
  25. Zabala, A., M. R. Martin, A. I. Haza, L. Fernandez, J. M. Rodriguez, and P. Morales. 2001. Antiproliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line. Lett. Appl. Microbiol. 32: 287-292 https://doi.org/10.1046/j.1472-765X.2001.00910.x