Reproductive and Developmental Biology

  • 제32권3호
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  • Pages.193-198
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  • 2008
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

돼지에 있어서 정소 생식세포의 이식 기법 개발

Development of Techniques for Testicular Germ Cell Transplantation in Pigs

  • 김병각 (중앙대학교 동물자원과학과) ;
  • 이용안 (중앙대학교 동물자원과학과) ;
  • 김방진 (중앙대학교 동물자원과학과) ;
  • 김기중 (중앙대학교 동물자원과학과) ;
  • 민관식 (한경대학교 생물환경정보통신전문대학원) ;
  • 이장희 (주)바이오컬쳐) ;
  • 유재원 (농촌진흥청 축산과학원) ;
  • 김인철 (농촌진흥청 축산과학원) ;
  • 류범용 (중앙대학교 동물자원과학과)
  • Kim, Byung-Gak (Department of Animal Science & Technology, Chung-Ang University) ;
  • Lee, Yong-An (Department of Animal Science & Technology, Chung-Ang University) ;
  • Kim, Bang-Jin (Department of Animal Science & Technology, Chung-Ang University) ;
  • Kim, Ki-Jung (Department of Animal Science & Technology, Chung-Ang University) ;
  • Min, Kwan-Sik (Animal Biotechnology, Graduate School of Bio. & Information Technology, HanKyang National University) ;
  • Lee, Jang-Hee (Bioculture Inc.) ;
  • Ryu, Jae-Weon (National Institute of Animal Science, RDA) ;
  • Kim, In-Cheul (National Institute of Animal Science, RDA) ;
  • Ryu, Buom-Yong (Department of Animal Science & Technology, Chung-Ang University)
  • 발행 : 2008.09.30
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초록

본 연구는 돼지에 있어서 정원줄기세포를 포함하는 정소세포를 recipient 돼지의 정소 내로 이식할 수 있는 기법을 개발하기 위하여 시행되었다. 공여세포는 $10{\sim}14$일령의 돼지로부터 채취된 정소에서 효소처리법을 이용하여 회수하였고, recipient의 정소 내로 이식하기 전 형광 마커(PHK26)로 표지하였다. 외과적 수술을 통하여 recipient 돼지부터 정소를 꺼낸 후 초음파 기기와 이식 장치를 이용하여 형광표지된 공여세포를 recipient 정소의 세정관 내로 이식하였다. 14주령의 recipient 정소에 $5{\sim}7ml$의 공여 세포부유액을 주입하여 정소 내 50% 이상의 세정관 내로 새포부유액의 주입이 가능하였고, 세포부유액이 주입된 세정관 내에서 형광표지된 정소세포들이 고루 이식되어짐이 관찰되었다. 본 연구에서 개발한 이식 기법을 이용하여 효율적인 정소세포의 이식이 가능함에 따라 향후 돼지 정원줄기세포의 연구 및 활용법 개발에 획기적인 돌파구가 마련될 것으로 기대된다.

The current study was designed to extend the technique of spermatogonial transplantation to economically important pig model We evaluated the efficiency of pig to pig transplantation. Donor testis cells were harvested from testes obtained at castration of 10- to 14-day-old boars and were labeled with fluorescent marker(PKH26) before transplantation. The presence of infused dye or labeled pig testicular cells was confirmed in the seminiferous tubules from recipient pig. The most effective procedure of intratubular germ cell transfer was to insert an fine needle ($21{\sim}25$ gauge) through the cauda epididymis and testis into the rete testis under ultrasound guidance. Infusion of $5{\sim}7ml$ of dye solution or cell suspension could fill the rete and up to 50% of seminiferous tubules of 14-week-old boars. Testis were examined for the presence and localization of labeled donor cells immediately after transplantation and labeled donor cells were found in numerous seminiferous tubules from recipient pig testes. These results indicate that germ cell transplantation is feasible in recipient pig testis. This study represents successful spermatogonial transplantation between individual animals in a livestock species.

키워드

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