Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Porcine Epididymal Fluid on In Vitro Maturation of Porcine Germinal Vesicle Oocyte

  • Yim, Cha-Ok (Department of Molecular Biology, Dong-Eui University) ;
  • Kim, Kyoung-Woon (Department of Molecular Biology, Dong-Eui University) ;
  • Kim, Byung-Ki (Department of Molecular Biology, Dong-Eui University)
  • Published : 2008.09.30
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Abstract

The aim of this study was to investigate what components of porcine epididymal fluid (pEF) influences the nuclear maturation of porcine germinal vesicle oocytes. Porcine cumulus-oocytes complexes from follicles were cultured in TCM 199 containing pEF. After 48 h cultures, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. Maturation rate of oocytes was significantly increased in media supplemented with 10% pEF during in vitro maturation (IVM) than in those without pEF. When lipid component of pEF was removed by treating n-heptane, no significant difference was observed in maturation of oocytes between n-heptane treatrment and intact pEF group. However, the proportion of oocytes reaching at metaphase II (M II) was significantly (p<0.05) decreased in the oocytes cultured in media containing trypsin-treated pEF compared to those in media with intact pEF. When porcine GV oocytes were matured in the medium supplemented with intact pEF or pEF heated at $56^{\circ}C$ and $97^{\circ}C$, rates of oocytes remained at GV stage were 11.7%, 29.4% and 42.0%, respectively. However, there were no difference in proportion of oocytes reaching at MII stage among intact pEF group and $56^{\circ}C$ group. Present study suggests that 1) pEF contains an enhancing component(s) for nuclear maturation in vitro of oocytes, 2) protein(s) of pEF may be capable to promote nuclear maturation in vitro, and 3) enhancing component for nuclear maturation may consist of two factors, which are responsible for germinal vesicle breakdown (GVBD) and promotion of MII stage.

Keywords

References

  1. Abeydeera LR, Wang WH, Randall S, Prather RS, Day BN (1998): Maturation in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro. Biol Reprod 58:1316-1320 https://doi.org/10.1095/biolreprod58.5.1316
  2. Antczak M, Van Blerkom J, Clark A (1997): A novel mechanism of vascular endothelial growth factor, leptin and transforming growth factor-beta 2 sequestration in a subpopulation of human ovarian follicle cells. Hum Reprod 12:2226-2234 https://doi.org/10.1093/humrep/12.10.2226
  3. Byscov AG, Andersen C, Leonarfsen L, Baltsen M, (1999): Meiosis activationg sterols (MAS) and fertility in mammals and man. Mol Dev Evol 285: 237-242 https://doi.org/10.1002/(SICI)1097-010X(19991015)285:3<237::AID-JEZ6>3.0.CO;2-S
  4. Byskov AG, Andersen CY, Nordholm L, Thogersen H, Xia G, Wassmann O, Andersen JV, Guddal E, Roed T (1995): Chemical structure of sterols that activate oocyte meiosis. Nature 374:559-562 https://doi.org/10.1038/374559a0
  5. Dacheux JL, Dacheux F, Paquignon M (1989): Changes in sperm surface membrane and luminal protein fluid content during epididymal transit in the boar. Biol Reprod 40:635-651 https://doi.org/10.1095/biolreprod40.3.635
  6. Down SM, Schroeder AC, Eppig JJ (1986): Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing the hardening of zona pellucida. Gamete Res 15:115-122 https://doi.org/10.1002/mrd.1120150203
  7. Edwards RG (1965): Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature 208:349-351 https://doi.org/10.1038/208349a0
  8. Farhi J, Nahum H, Zakut H, Levran D (1997): Incubation with sperm enhances in vitro maturation of the oocyte from the germinal vesicle to the M2 stage. Fertil Steril 68:318-322 https://doi.org/10.1016/S0015-0282(97)81522-1
  9. Fouchecourt S, Dacheux F, Dacheux JL (1999): Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids : Origin and regulation. Biol Reprod 60:558-566 https://doi.org/10.1095/biolreprod60.3.558
  10. Goto K, Kajihara Y, Kosaka S, Koba M, Nakanishi Y, Ogawa K (1998): Pregnancies after co-culture of cumulus cell with bovine embryos derived from in vitro fertilization of in vitro matured follicular oocytes. J Reprod Fertil 83:753-758
  11. Goud PT, Goud AP, Qian C, Laverge H, van der Elst J, Sutter PD, Dhont M (1998): In-vitro maturation of human germinal vesicle stage oocytes: role of cumulus cells and epidermal growth factor in the culture medium. Hum Reprod 13:1638- 1644 https://doi.org/10.1093/humrep/13.6.1638
  12. Henault MA, Killian GJ, Kavanaugh JF, Griel LC (1995): Effect of accessory sex gland fluid from bulls of differing fertilities on the ability of cauda epididymal sperm to penetrate zona-free bovine oocytes. Biol Reprod 52:390-397 https://doi.org/10.1095/biolreprod52.2.390
  13. Hyttel P, Xu KP, Smith S, Greve T (1986): Ultr maturation in superovulated cattle. J Reprod Fertil 78: 615-625 https://doi.org/10.1530/jrf.0.0780615
  14. Kim BK, Jabed MA, Kang SR, Kim DE, Han CH, Huh MK, Tania K (2008): Effects of spermatozoa during in vitro meiosis progression in the porcine germinal vesicle oocytes. Anim Reprod Sci 104:83-92 https://doi.org/10.1016/j.anireprosci.2007.01.015
  15. Legge M (1995): Oocyte and zygote zona pellucida permeability to macromolecules. J Exp Zool 271: 145-150 https://doi.org/10.1002/jez.1402710210
  16. Metayer S, Dacheux JL, Dacheux F, Gatti JL (2002): Comparison, characterization and identification of proteases and protease inhibitors in epididymal fluid of domestic mammals. Matrix metalloproteinases are major fluid gelatinases. Biol Reprod 66:1219-1229 https://doi.org/10.1095/biolreprod66.5.1219
  17. Nagai T, Bing Y, Rodriguez-Martinez H (2000): Effect of cysteamine and FSH on in vitro maturation of porcine oocytes. Theriogenology 53:461
  18. Pincus G, Enzmann EV (1935): The comparative behavior of mammalian eggs in vivo and in vitro. 1. The activation of ovarian eggs. J Exp Med 62: 665-675 https://doi.org/10.1084/jem.62.5.665
  19. Russell LD, Peterson RN, Hunt W, Strack LE (1984): Post testicular surface modification and contributions of reproductive tract fluids to the surface polypeptide composition of boar spermatozoa. Biol Reprod 30:959-978 https://doi.org/10.1095/biolreprod30.4.959
  20. Syntin P, Dacheux F, Druart X, Gatti JL, Okamura N, Dacheux JL (1996): Characterization and identification of proteins secreted in the various regions of the adult boar epididymis. Biol Reprod 55: 956-974 https://doi.org/10.1095/biolreprod55.5.956
  21. Vanderhyden BC, Armstrong DT (1990): Effect of gonadotropins and granulosa cell secretions on the maturation and fertilization of rat oocytes in vitro. Mol Reprod Dev 26:337-46 https://doi.org/10.1002/mrd.1080260408
  22. Vatzias G, Hagen DR (1999): Effects of porcine follicular fluid and oviduct-conditioned media on maturation and fertilization of porcine oocytes in vitro. Biol Reprod 60:42-48 https://doi.org/10.1095/biolreprod60.1.42
  23. Yim CO, Lee SM, Kim HR, Jabed MA, Lee CB, Kim BK(2006): Stimulatory effect of porcine epididymal fluid on in vitro maturation of porcine immature oocytes. Reprod Dev Biol 30(4):301-305
  24. Yoshida M, Bamba K, Kojima Y (1989): Effects of gonadotropins and estradiol-17$\beta$ on the timing of nuclear maturation and cumulus mass expansion in pig oocytes cultured in vitro. Jpn J Animl Reprod 35:86-91 https://doi.org/10.1262/jrd1977.35.86
  25. Yoshimura Y, Hosei Y, Iritani A, Nakamura Y, Atlas SJ, Wallach EE (1989): Developmental potential of rabbit oocytes matured in vitro: the possible contribution of prolactin. Biol Reprod 40:26- 33
  26. Zheng YS, Sirard MA (1992): The effect of sera, bovine serum albumin and follicular cells on in vitro maturation and fertilization of porcine oocytes. Theriogenology 37:779-790 https://doi.org/10.1016/0093-691X(92)90041-O