DOI QR코드

DOI QR Code

옥수수의 steam flake 처리가 in situ 건물소화율 및 저장기간별 aflatoxin과 ochratoxin 함량에 미치는 영향

Effects of Steam Flaking on In situ DM Digestibility and Aflatoxin and Ochratoxin Contents during Storage of Corns

  • 이신자 (경상대학교 응용생명과학부(BK 21)) ;
  • 이지훈 (경상대학교 응용생명과학부(BK 21)) ;
  • 신년학 (경상대학교 응용생명과학부(BK 21)) ;
  • 현종환 (경상대학교 응용생명과학부(BK 21)) ;
  • 문여황 (진주산업대학교 동물생명과학과) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 이성실 (경상대학교 응용생명과학부(BK 21))
  • Lee, Shin-Ja (Division of Applied Life Science(BK 21 Program), Graduate School of Gyeongsang National University) ;
  • Lee, Ji-Hun (Division of Applied Life Science(BK 21 Program), Graduate School of Gyeongsang National University) ;
  • Shin, Nyeon-Hak (Division of Applied Life Science(BK 21 Program), Graduate School of Gyeongsang National University) ;
  • Hyun, Jong-Hwan (Division of Applied Life Science(BK 21 Program), Graduate School of Gyeongsang National University) ;
  • Moon, Yea-Hwang (1Department of Animal Sci. & Biotech., Jinju National University,) ;
  • Lee, Sang-Suk (Department of Animal Science & Biotechnology, Sunchon National University) ;
  • Lee, Sung-Sill (Division of Applied Life Science(BK 21 Program), Graduate School of Gyeongsang National University)
  • 발행 : 2008.11.30

초록

본 연구는 옥수수 수입원과 steam flake 처리에 따른 in situ 건물 분해율 및 보관기간별 mycotoxins 함량을 조사하기 위하여 수행되었다. 미국과 인도산 옥수수(yellow dent corn)를 공시재료로 하여, steam flake 처리 효과에 대해 조사하였다. 시험구는 총 4개의 처리구(USCW, 미국산 무처리 옥수수; USCF, 미국산 steam flaking 옥수수; IDCW, 인도산무처리 옥수수; IDCF, 인도산 steam flaking 옥수수)를 두고, 각 처리구당 보관기간(0, 1, 2, 3, 4, 5, 6, 7, 8주)별 4반복으로 수행되었다. In situ 시험을 위해서 반추위 누관이 장착된 Holstein 수소 2두가 이용되었고, 공장환경분야 표준상태 (STP, standard temperature and pressure, $25^{\circ}C$ 1 atm.)에서 보관기간 동안 매주, 곰팡이 독소인 aflatoxin과 ochratoxin의 농도를 측정하였다. 옥수수의 반추위 내 발효시간별 in situ 건물 소실율은 무처리구에서는 미국산이 인도산보다 약 $3.0{\sim}44.1%$ 높았으나, steam flaking 처리를 함으로써 건물소실율의 차이가 없어졌다. 미국산 옥수수는 무처리 알곡의 건물 분해율이 높은 편이었으나, flake처리에 의한 영향이 적은 반면, 인도산 옥수수는 무처리 알곡의 건물 분해율은 낮은 편이지만 flake 처리를 하였을 때, 분해율이 크게 증가되었다. 미국산 옥수수와 flake 처리 옥수수의 aflatoxin 함량은 보관기간 동안 매우 낮은 수준이었으나, 인도산 옥수수 알곡을 6주 이상 보관할 경우, 허용기준치를 초과하였다. 따라서 인도산 옥수수 알곡을 장기보관 해야 할 경우, steam flake 처리를 하는 것이 aflatoxin 오염방지를 위해서 필수적이며, 알곡으로 보관할 경우는 6주 이상을 넘기지 않는 것이 중요할 것으로 사료된다.

This study was conducted to investigate the effects of steam flaking of corn grains on in situ dry matter degradability in the rumen and contents of mycotoxins (aflatoxin, ochratoxin) during storage. Yellow dent corns imported from USA and India were flaked, and stored for 8 weeks under the standard temperature and pressure (STP; $25^{\circ}C$/ 1 atm.). Experimental treatments were composed of four corn grains (untreated-USA corn, USCW; steam flaked-USA corn, USCF; untreated-India corn, IDCW; steam flaked-India corn, IDCF) with 4 replications according to 9 storage periods (0, 1, 2, 3, 4, 5, 6, 7, 8 week). Two ruminally cannulated Holstein bulls were used for in situ trial. Pathogen contamination trial was performed by comparing the mycotoxin contents in corns during storage periods. Dry matter disappearance rate in the rumen was about 3.0 to 44.1% higher (P<0.05) for USCW than IDCW, but was not difference between USCF and IDCF. With steam flaking of corn, dry matter degradability in the rumen was significantly (P<0.05) increased in corn from India, but was not affected in corn from USA. Aflatoxin content was very low level in corns from USA and steam flaked corns, but was higher than the tolerance limit of domestic aflatoxin content regulation when IDCW was stored over 6 week under STP. Ochratoxin content was low level in all treatments. From above results, it is reasonable that the corn imported from India might be flaked for enhance the ruminal DM degradability and safe from aflatoxin pathogen.

키워드

참고문헌

  1. A.O.A.C. 1995. Official methods of analysis 16th edition. Association of official analytical chemists, Washington, D.C.
  2. Aimone, J. C. and D. G. Wagner. 1977. Micronized wheat II. Influences on in vitro disappearance rate, in vitro gas production and gelatinization. J. Anim. Sci. 44, 1096-1099. https://doi.org/10.2527/jas1977.4461096x
  3. Banks, W. and C. T. Greenwood. 1975. The hydrodynamic behaviour of native amylose. In: Conformation of Biopolymers. Vol. 2. pp. 739, Academic Press, London.
  4. Beeson, W. M. 1972. Effects of steam flaking, roasting, popping and extrusion of grains on their nutritional value for beef cattle. Proc. Symposium "Effects of processing on nutritional value of feeds". pp. 326-335, N. A. S. Washington DC.
  5. Bullerman, L. B. 1979. Significance of mycotoxins to food safety and human health. J. Food Prot. 42, 65-86. https://doi.org/10.4315/0362-028X-42.1.65
  6. Cho, J. H., I. K. Paik and Y. Hyun. 2007. Comparison of TME values of imported corns and ME values determined by In vitro method. J. Anim. Sci. & Technol. (Kor.) 49, 737-74. https://doi.org/10.5187/JAST.2007.49.6.737
  7. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11, 1. https://doi.org/10.2307/3001478
  8. Ehle, F. R. 1984. Influence of particle size on determination of fibrous feed components. J. Dairy Sci. 67, 1482-1488. https://doi.org/10.3168/jds.S0022-0302(84)81465-4
  9. Ehle, F. R., M. R. Murphy and J. H. Clark. 1982. In situ Particle size reduction and the effect of particle size on degradation of crude protein and dry matter in the rumen of dairy steers. J. Dairy Sci. 65, 963-971. https://doi.org/10.3168/jds.S0022-0302(82)82297-2
  10. Firkins, J. L., M. L. Eastridge, N. R. St-Pierre and S. M. Noftsger. 2001. Effects of grain variability and processing on starch utilization by lactating dairy cattle. J. Anim. Sci. 79, 218-238. https://doi.org/10.2527/jas2001.79E-SupplE218x
  11. Ganesh, D. and D. G. Grieve. 1990. Effect of roasting row soybeans at three temperatures on in situ dry matter and nitrogen disaddearance in dairy cows. J. Dairy Sci. 73, 3222-3230. https://doi.org/10.3168/jds.S0022-0302(90)79014-5
  12. Ha, J. K., S. S. Lee, B. O. Kwak, T. H. Moon and S. H. Kang. 1994. Effects of corn processing methods on the nutrients utilization I. Disappearances and degradabilities of dry matter and crude protein of deedstuffs in the rumen of Holstein cows. Korean J. Dairy Sci. 16, 1-17.
  13. Hale, W. H. 1973. Influence of processing on the utilization of grains (strarch) by ruminants. J. Anim. Sci. 37, 1075-1080. https://doi.org/10.2527/jas1973.3741075x
  14. Hale, W. H., L. Cuitun, W. J. Soba and B. Theurer. 1967. Effect of steam processing and flaked milo and barley on performance and digestion by steers. J. Anim. Sci. 25, 392-396.
  15. Kamimura, H. 1993. Problems with mycotoxin in food sanitation. pp. 10, Hyogo International Center Japan International Cooperation Agency.
  16. Kim, N. S., J. K. Ha, Y. G. Ko, H. D. Kim, W. Y. Kim and B. O. Kwak. 1996. Effects of corn processing methods on the nutrient utilization. II. Effects of processed corn on ruminal degradability of starch and lower gut disappearance rates of dry matter and crude protein in holstein dairy cows. Kor. J. Anim. Nutr. Feed. 20, 360-370.
  17. Kim, T. M., Y. M. Cho, Y. S. Song, M. J. Seong, J. H. Kim, S. J. Lee, C. B. Park, J. T. Hong, D. J. Kim, J. K. Kang and Y. W. Yun. 2002. Relative toxicities of aflatoxins for carcinogenic risk assessment. The Annual Report of KNTP 1, 127-143.
  18. Kim, Y. K. and F. N. Owens. 1985. Starch digestion by feedlot cattle: Influence of roughage and intake level and particle size. Oka. Agric. Exp. Stat. Anim. Sci. Res. Rep. 117, 298-302.
  19. Kinsler, T. W., E. F. Davis and M. G. Bolton. 1993. Strategies for chemoprevention against aflatoxin-induced liver cancer. pp 281-306. In: ends, by Eaton DL, Groopman JD. The Toxicology of Aflatoxins. Academin Press. New York, USA.
  20. Lee, S. M., T. W. Kang, S. J. Lee, J. U. Ok, Y. H. Moon and S. S. Lee. 2006. Studies on In situ and In vitro Degadabilities, Microbial Growth and Gas Production of Rice, Barley and Corn. J. Anim. Sci. & Technol. (Kor.) 48, 699-708. https://doi.org/10.5187/JAST.2006.48.5.699
  21. Lee, R. W., M. L. Galyean and G. P. Lofgreen. 1982. Effects of mixing whole shelld and steam-flaked corn in finishing diets on feedlot performance and site and extent of digestion in beef steers. J. Anim, Sci. 55, 475-583. https://doi.org/10.2527/jas1982.553475x
  22. Ministry of Agriculture. 1993. Feed manufacturing Industry status.
  23. Ministry of Agriculture. 2005. Feed management statute book. pp. 222.
  24. Moe, P. W. and H. F. Tyrrell. 1977. Effects of feed intake and physical form on energy value of corn in timothy hay diets for lactating cows. J. Dairy Sci. 60, 752-758. https://doi.org/10.3168/jds.S0022-0302(77)83930-1
  25. Namkung, H., I. K. Paik and H. S. Lee. 1993. Determination of amino acid availability and metabolizable energy in Protein feedstuffs by true amino acid availability (TAAA) method. K. J. Poult. Sci. 20, 189-196.
  26. Nocek, J. E. 1988. In situ and other methods to estimate ruminal protein and energy disappearance rate, A review. J. Dairy Sci. 71, 2051-2069. https://doi.org/10.3168/jds.S0022-0302(88)79781-7
  27. NRC. 1984. Ruminant nitrogen usage (6th Eds.). National Academy Press, Washington, D.C., USA.
  28. Orskov, E. R. and I. Mcdonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92, 499-503. https://doi.org/10.1017/S0021859600063048
  29. Park, C. H, H. Y. Oh, O. S. Heo, S. J. Sohn, E. S. Han, J. W. Kim and M. O. Eom. 1997. Safety evaluation of natural toxin. The annual report of Korea food & drug administration. 1, 431.
  30. Perry, T. W., L. M. Hammond, R. C. Peterson and W. M. Beeson. 1970. Value of roasting corn for finishing heifers. Purdue Res. Prog. Rep. Perdue Univ. Agric. Exp. Sta. 379.
  31. Philippeau, C., F. Le Deschault de Monredon and B. Michalet-Doreau. 1999. Relationship between ruminal starch degradation and the physical characteristics of corngrain. J. Anim. Sci. 77, 238-243.
  32. Poppi, D. P., B. W. Norton, D. J. Minson and R. E. Hendrichson. 1980. The validity of the critical size theory for particles leaving the rumen. J. Agric. Sci. 94, 275-280. https://doi.org/10.1017/S0021859600028859
  33. Rahnema, S. H., B. Theurer, J. A. Garcia, W. H. Hale and M. C. Young. 1987. Site of protein digestion in steers fed sorghum grain diets. II. Effects of grain processing methods. J. Anim. Sci. 64, 1541-1547. https://doi.org/10.2527/jas1987.6451541x
  34. Ramirez, R. G., H. E. Kiesling, M. L. Galyean, G. P. Lofgreen and J. K. Elliott. 1985. Influence of steam-flaked, steamed-whole or whole shelled corn on performance and digestion in beef steers. J. Anim. Sci. 61, 1-8.
  35. Rooney, L. W. and R. L. Pflugfelder. 1986. Factors affecting starch disappearance rate with special emphasis on sorghum and corn. J. Anim. Sci. 63, 1607-1623. https://doi.org/10.2527/jas1986.6351607x
  36. Rooney, L. W., M. N. Khan. and C. F. Earp. 1980. The technology of sorghum products. pp. 513-554, In Inglett, G. (eds.), Recent progress in cereal chemistry: Cereals for food and Beverages. Academic Press. NY. USA.
  37. SAS. 1999. SAS/STAT guide for personal computers @8.01. SAS Inst., Gary., NC., USA.
  38. Schwarzenbach, R. P., P. M. Gschwend and D. M Imboden. 1993, Environmental Organic Chemistry. pp. 681. John Willey & Sons, New York.
  39. Shin, K. S., H. S. Shin, Y. W. Lee and Y. C. Jung. 1985. The Newest Feed Hygienics. pp. 171, The publishing company Shin Kwang. Seoul.
  40. Smith, J. E. and M. O. Moss. 1985. Mycotoxins, formation, analysis and significance. pp. 148, New York: John Wiley & Sons.
  41. Son, K. N., Y. K. Kim, S. K. Lee and H. S. Kim. The effects of processing methods of corn on In vitro DM digestability and in sacco degradability in the rumen. 2003. J. Anim. Sci. & Technol. (Kor.) 45, 433-442. https://doi.org/10.5187/JAST.2003.45.3.433
  42. Tamminga, S., P. Van der Togt, C. J. Van der Koelen, C. Melieftste, M. Luttikuis and G. D. H. Classon. 1989. The behavior of starch in the rumen of dairy cows. Modeling No. 14. Inst. Livest. Feeding Nutri. Lelystad. Netherlands.
  43. Theurer, C. B., O. Lozano, A. Alio, A. Delgado-Elorduy, M. Sadik, J. T. Huber and R. A. Zinn. 1999. Steam-processed corn and sorghum grains flaked at different densities alter ruminal, small intestinal, and total tract disappearance rate of starch by steers. J. Anim. Sci. 77, 2824-2831. https://doi.org/10.2527/1999.77102824x
  44. Thomas, E. E. 1988. The effect of particle size and steam treatment of feedstuffs on rate and extent of digestion (in vitro and in situ). J. Anim. Sci. 66, 243-249. https://doi.org/10.2527/jas1988.661243x
  45. Van Keuren, R. W. and W. W. Heineman. 1962. Study of a nylon bag technique for in vivo estimation of froage disappearance rate. J. Anim. Sci 21, 340-345. https://doi.org/10.2527/jas1962.212340x
  46. Van Soest, P. V. 1982. Carbohydratee. pp. 108, Nutritional Ecology of the Ruminants. O&Books, INC., Corrallis. OR, USA.
  47. Verite, R., B. Michalet-Doreau, P. Chapoutot, J. L. Peyraud and C. Poncet. 1987. Vision Du systmed des protines digesstible dans l'intestin (PDI). Bull. Tech. CRZV. Theix, INRA, 70, 19-34.
  48. Wall, J. S. and L. W. Paulis. 1978. Corn and sorghum grain proteins. pp. 135-219, In Pomeranz, Y. (ed.) Advances in Cereal Science and Technology II. Am. Assoc. Cereal Chem., St. Paul. MN, USA.
  49. Whister, R. L. and J. R. Daniel. 1984. Molecular structure of atarch. pp. 153-182, In : Chemistry and TEchnology (2nd Ed.). Academic Press. NY, USA.
  50. Wikipedia$\circledR$, $2008^a$. Aflatoxin A., Wikimedia Foundation, Inc., a U.S..
  51. Wikipedia$\circledR$, $2008^b$. Ochratoxin A., Wikimedia Foundation, Inc., a U.S..
  52. Zinn, R. A. and F. N. Owens. 1983. Influence of feed intake level on site of digestion in steers fed a high concentrate diet. J. Anim. Sci. 56, 471-475. https://doi.org/10.2527/jas1983.562471x