생명과학회지 (Journal of Life Science)

  • 제18권11호
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  • Pages.1479-1484
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해양심층수의 해마신경세포 연접형성 촉진 효과

Promotion of Synaptic Maturation by Deep Seawater in Cultured Rat Hippocampal Neurons

  • 김성호 (동국대학교 의과대학 내과학교실) ;
  • 이현숙 (동국대학교 의과대학 해부학교실) ;
  • 손윤희 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연 구센터 및 의과대학 약리학교실) ;
  • 남경수 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연 구센터 및 의과대학 약리학교실) ;
  • 문일수 (동국대학교 의과대학 해부학교실)
  • Kim, Seong-Ho (Department of Internal Medicine, Dongguk University) ;
  • Lee, Hyun-Sook (Department of Anatomy, College of Medicine, Dongguk University) ;
  • Shon, Yun-Hee (Regional Innovation System, Dongguk University) ;
  • Nam, Kyung-Soo (Regional Innovation System, Dongguk University) ;
  • Moon, Il-Soo (Department of Anatomy, College of Medicine Dongguk University)
  • 발행 : 2008.11.30
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초록

해양심층수(deep seawater, DSW)는 청정성, 풍부한 무기물질 및 필수미량원소의 함유로 음료를 포함한 여러 분야에 응용하기 위하여 최근 많은 관심을 받고 있다. 본 연구실에서는 동해 양양 부근의 해저 1,100 m에서 취수하여 역삼투압 시스템으로 탈염과 농축을 한 심층수가 경도 600-1,000에서 배양한 흰쥐해마신경세포의 형태적 분화에 도움을 줌을 보고한 바 있다. 좀 더 구체적인 영향을 조사하기 위하여 본 연구에서는 경도 800 및 1,000 심층수가 25%(v/v) 포함된 minimal essential media에서 배양한 해마신경세포에서의 연접형성을 대조군(증류수 첨가)과 비교하였다. 경도 800 및 1000에서 자란 신경세포는 흥분성 연접후세포막의 신호전달복합체인 NRC/MASC 크러스터의 형성을 촉진하였다. 또한 연접전/후 구조가 밀접히 짝을 이룬 연접이 매우 현저히(p<0.01) 증가하였다. 이 결과는 심층수가 연접의 형성을 촉진하여 신경세포의 건강에 도움을 주는 것으로 해석된다.

Deep seawater (DSW) refers to water extracted from the ocean, usually at depths of 200 meters or more, which is rich in inorganic materials and has attracted attention for various applications. We investigated the effects of the DSW on the synaptic maturation of cultured rat hippocampal neurons. Immunocytochemical examination of DIV21 showed that PSD-95, $\alpha$CaMKII, and synGAP$\alpha1$clusters were strengthened and coupling rates of SV2 and NR2B were significantly increased in neurons grown in the presence of H-800 and H-1000 DSW. Our results indicate that DSW promotes the formation of excitatory postsynaptic signal transduction complexes NRC/MASC and functional synapses.

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