Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Detection of Microbial Growth in an Automated Culture System

자동배양기를 이용한 미생물 검출

  • Sung, Hye-Ran (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University) ;
  • Kim, Il-Hoi (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University) ;
  • Kim, Jee-Youn (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University) ;
  • Lee, Chong-Kil (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University) ;
  • Chung, Yeon-Bok (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University) ;
  • Han, Sang-Bae (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University) ;
  • Song, Suk-Gil (National Research Laboratory of PK/PD, CBITRC, College of Pharmacy, Chungbuk National University)
  • 성혜란 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단) ;
  • 김일회 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단) ;
  • 김지연 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단) ;
  • 이종길 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단) ;
  • 정연복 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단) ;
  • 한상배 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단) ;
  • 송석길 (충북대학교 약학대학 약동력학 국가지정연구실, 충북 BIT 연구중심대학 육성사업단)
  • Published : 2008.06.30
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Abstract

Modern automated culture systems have increased the isolation rate of microorganisms and shortened the time to detection, reducing experimental errors in diagnosis of infecting agents. BacT/ALERT 3D system is based on the colorimetric detection of $CO_2$ produced by the growing microorganisms. In order to evaluate the efficiency of the detection system, sterility test were performed using 6 bacteria. With standard aerobic and anaerobic bottles containing the liquid media, both three aerobic bacteria (P. aeruginosa, M. luteus, B. subtilis) and a facultative bacterium S. aureus were detected up to 1 CFU in 31.44 hr. In addition, growth of anaerobic C. sporogenes was recognized up to 1 CFU in 15.96 hr. The slowly growing bacteria P. acnes was detected up to 10,000 CFU in 129.36 hr. In comparison with conventional culture method, BacT/ALERT 3D automated culture system was more sensitive and saved detection time up to$2\sim10$ hr. Therefore, this automated culture system enables to efficiently detect bacteria in clinical samples and biological medicines.

미생물 자동배양기를 이용한 감염성 물질의 검출은 시험적 오차의 경감은 물론 분리율 향상과 시간 단축을 가능하게 하였다. BacT/ALERT 3D 자동배양기는 미생물성장 시 발생되는 이산화탄소를 비색법으로 검출하는 장비로서 임상시료를 이용한 미생물 배양과 검출에 이용되어왔다. 자동배양기의 효율성을 검증하고 의약품의 무균 시험에 적용가능한지를 평가하기 위하여 6종의 세균을 이용하여 중식 및 검출특성을 분석하였다. 3종의 호기성세균 Pseudomonas aeruginosa, Micrococcus Iuteus, Bacillus subtilis와 Staphylococcus aureus는 1 CFU가 31.44 시간 내에 검출되었고, 혐기성균인 Clostridium sporogenes는 15.96시간에 균의 중식이 감지되었다. 저성장 혐기성세균인 Propionibacterium acnes는 $10^4$ CFU의 균수에서 검출에 129.36시간이 소요되었다. 이 같은 결과는전통적 직접 배양법보다 검출감도에 있어서 $10\sim10^5$배 높고 검출시간을 $2\sim10$10시간 단축한 것으로서 자동배양법이 미생물 증식과 검출에 효율적임을 말해준다. 따라서 본 시험에서 이용한 자동배양법은 임상시료에서의 감염원 진단뿐 아니라 생물의약품의 무균시험에 이용 가능하다고 판단된다.

Keywords

References

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