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Incidence of Coleus blumei viroid 1 in Seeds of Commercial Coleus in Korea

  • Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Gug-Sun (National Horticultural Research Institute, Rural Development Administration)
  • Published : 2008.09.30

Abstract

A viroid was detected from symptomless Coleus blumei cultivar 'Kong Scarlet'. It consisted of 249 nucleotides(GenBank accession no. EU410620), which was 100% identical to a Coleus blumei viroid 1(CbVd 1) reported from China(GenBank accession no. DQI78399), indicating that the viroid was an isolate of CbVd1. Attempts were made to determine if commercial Coleus seeds were infected with CbVd. Infection rates in seedlings of the 14 commercial cultivars of Coleus ranged from 0 to 100%. CbVd1 caused discoloration and growth retardation in some cultivars, but is symptomless in others. These results indicated that Coleus in commercial markets in Korea is highly infected with CbVd.

Keywords

References

  1. Chung, B. N., Kim, D. C., Kim, J. S. and Cho, J. D. 2006. Occurrence of Chrysanthemum chlorotic mottle viroid in chrysanthemum (Dendranthema grandiflorum) in Korea. Plant Pathol. J. 23:334-338 https://doi.org/10.5423/PPJ.2006.22.4.334
  2. Chung, B. N. and Pak, H. S. 2008. Seed transmission of Chrysanthemum stunt viroid in Chrysanthemum. Plant Pathol. J. 24:31-35 https://doi.org/10.5423/PPJ.2008.24.1.031
  3. Fonseca, M. E. N., Boiteux, L. S., Singh, R. P. and Kitajima, E. W. 1989. A small viroid in Coleus species from Brazil. Fitopatol. Braz. 14:94-96
  4. Fonseca, M. E. N., Marcellino, L. H., Kitajima, E. W. and Boiteux, L. S. 1994. Nucleotide sequence of the original Brazilian isolate of coleus yellow viroid from Solenostemon scutellarioides and infectivity of its complementary DNA. J. Gen. Virol. 75:1447-1449 https://doi.org/10.1099/0022-1317-75-6-1447
  5. Ishiguro, A., Sano, T. and Harada, Y. 1996. Nucleotide sequence and host range of coleus viroid isolated from coleus (Coleus blumei Benth.) in Japan. Ann. Phytopathol. Soc. Jpn. 62:84-86 https://doi.org/10.3186/jjphytopath.62.84
  6. Li, S. F., Su, Q. Guo, R., Tsuji, M. and Sano, T. 2006. First report of Coleus blumei viroid from coleus in China. P. Pathol. 55:565
  7. Shiwaku, K., Iwai, T. and Yamamoto, Y. 1996. Cloning and nucleotide sequence of Chrysanthemum stunt viroid. Hyogo Pre. Agric. Inst. (Agriculture) 44:1-4
  8. Singh, R. P. 1970. Seed transmission of potato spindle tuber virus in tomato and potato. Amer. Potato. J. 47:225-227 https://doi.org/10.1007/BF02872303
  9. Singh, R. R. and Boucher, A. 1991. High incidence of transmission and occurrence of a viroid in commercial seeds of Coleus in Canada. Plant Dis. 75:184-187 https://doi.org/10.1094/PD-75-0184
  10. Spieker, R. L. 1996a. A new sequence variant of Coleus blumei viroid 1 from the Coleus blumei cultivar 'Rainbow Gold'. Arch. Virol. 141:2153-2161 https://doi.org/10.1007/BF01718222
  11. Spieker, R. L. 1996b. In vitro-generated 'inverse' chimeric Coleus blumei viroids evolve in vivo into infectious RNA replicons. J. Gene. Virol. 77:2839-2846 https://doi.org/10.1099/0022-1317-77-11-2839
  12. Spieker, R. L., Haas, B., Charng, Y., Freimuller, K. and Sanger, H. L. 1990. Primary and secondary structure of a new viroid species (CbVd 1) present in the Coleus blumei cultivar 'Bienvenue'. Nucl. Acids Res. 18:3998 https://doi.org/10.1093/nar/18.13.3998

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