The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

Morphological Variations, Genetic Diversity and Pathogenicity of Colletotrichum species Causing Grape Ripe Rot in Korea

  • Hong, Sung-Kee (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration(RDA)) ;
  • Kim, Wan-Gyu (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration(RDA)) ;
  • Yun, Hae-Keun (Fruit Research Division, National Horticultural Research Institute, RDA) ;
  • Choi, Kyung-Jin (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration(RDA))
  • 발행 : 2008.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Ripe rot was frequently observed on fruits, leaves and stems of grape growing in eight locations in Korea from 2004 to 2006. All 30 isolates of Colletotrichum sp. were obtained from lesions of the ripe rot on grape plants. Out of the isolates, 19 isolates were identified as Colletotrichum acutatum and the others as Colletotrichum gloeosporioides based on morphological and cultural characteristics. Inter and intra specific variations of the Colletotrichum spp. isolates were investigated using RAPD and sequences of rDNA ITS and $\beta$-tubulin-2. Isolates of C. acutatum and C. gloeosporioides were distinctly differentiated by molecular analyses. Phylogenetic trees of ITS and$\beta$-tubulin-2 showed that Korean isolates of C. acutatum were clustered into groups A2 and A3 among the eight global groups. A2 included non-chromogenic isolates and A3 chromogenic ones. Both C. acutatum and C. gloeosporioides isolates were tested for pathogenicity to grape leaves. All isolates tested induced lesions on the leaves of grape by artificial inoculation. There was no difference in pathogenicity between C. acutatum and C. gloeosporioides isolates. This is the first report that C. acutatum except C. gloeosporioides causes grape ripe rot in Korea.

키워드

참고문헌

  1. von Arx, J. A. 1957. Die Arten der Gattung Colletotrichum Cda. Phytopathogische Zeitschrift 29:413-468
  2. Baxter, A. P., van der Westhuizen, G. C. A. and Eicker, A. 1983. Morphology and taxonomy of South African isolates of Colletotrichum. S. Afr. J. Bot. 2:259-289 https://doi.org/10.1016/S0022-4618(16)30090-0
  3. Cho, W. D. and Shin, H. D. eds. 2004. List of plant diseases in Korea. Fourth edition. The Korean Society of Plant Pathology. Suwon, Korea. 779 pp. (In Korean)
  4. Daykin, M. E. and Milholland, R. D. 1984. Ripe rot of muscadine grape caused by. Colletotrichum gloeosporioides and its control. Phytopathology 74:710-714 https://doi.org/10.1094/Phyto-74-710
  5. Dyko, B. J. and Mordue, J. E. M. 1979. Colletotrichum acutatum. CMI Descriptions of Pathogenic Fungi and Bacteria. No. 630. C.M. I., Kew, U.K
  6. Glass, N. L. and Donaldson, G. 1995. Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61:1323-1330
  7. Gunnell, P. S. and Gubler, W. D. 1992. Taxonomy and morphology of Colletotrichum species pathogenic to strawberry. Mycologia 84:157-165 https://doi.org/10.2307/3760246
  8. Johnston, P. R. and Jones, D. 1997. Relationships among Colletotrichum isolates from fruit-rots assessed using rDNA sequences. Mycologia 89:420-430 https://doi.org/10.2307/3761036
  9. Johnston, P. R. 2000. The importance of phylogeny in understanding host relationships within Colletotrichum. In: Colletotrichum. Host specificity, pathology and host-pathogen interaction. Prusky, D., Freeman, S. and Dickman eds. APS press, St. Paul, MN, USA
  10. Kang, H. W., Park, D. S., Go, S. J. and Eun, M. Y. 2003. Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice. Mol. Cells 13:1-7
  11. Kim, D. H., Jeon, Y. A., Go, S. J., Lee, J. K. and Hong, S. B. 2006. Reidentification of Colletotrichum gloeosporioides and C. acutatum isolates stored in Korea Agricultural Cultural Collection (KACC). Res. Plant Dis. 12:168-177. (In Korean) https://doi.org/10.5423/RPD.2006.12.3.168
  12. Kim, J. T., Park, S. Y., Choi, W. C., Lee, Y. H. and Kim, H. T. 2008. Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathol. J. 24:17-23 https://doi.org/10.5423/PPJ.2008.24.1.017
  13. Kim, S. H., Choi, S. Y., Lim, Y. S., Yoon, J. T. and Choi, B. S. 2001. Etiological characteristics and chemical control of ripe rot in grape cultivar Campbell Early. Res. Plant. Dis. 7:140-144 (In Korean)
  14. Kim, W. G. and Hong, S. K. 2008. Occurrence of anthracnose on peach tree caused by Colletotrichum species. Plant Pathol. J. 24:80-83 https://doi.org/10.5423/PPJ.2008.24.1.080
  15. Kim, W. G., Hong, S. K. and Park, Y. S. 2007. Occurrence of anthracnose on fruits of Asian pear tree caused by Colletotrichum acutatum. Mycobiology 35:238-240 https://doi.org/10.4489/MYCO.2007.35.4.238
  16. Lardner, R., Johnston, P. R., Plummer, K. M. and Pearson, M. N. 1999. Morphological and molecular analysis of Colletotrichum acutatum sensu lato. Mycol. Res. 103:275-285 https://doi.org/10.1017/S0953756298007023
  17. Lee, D. H., Kim, D. H., Jeon, Y. A., Uhm, J. Y. and Hong, S. B. 2007. Molecular and cultural characterization of Colletotrichum spp. causing bitter rot of apples in Korea. Plant Pathol. J. 23:37-44 https://doi.org/10.5423/PPJ.2007.23.2.037
  18. Martinez-Culebras, P. V., Querol, A., Suarez-Fernandez, M. B., Garcia-Lopez, M. D. and Barrio, E. 2003. Phylogenetic relationships among Colletotrichum pathogens of strawberry and design of PCR primers for their identification. J. Phytopathology. 151:135-143 https://doi.org/10.1046/j.1439-0434.2003.00694.x
  19. Melksham, K. J., Weckert, M. A. and Steel, C. C. 2002. An unusual bunch rot of grapes in sub-tropical regions of Australia caused by Colletotrichum acutatum. Australas. Plant Pathol. 31:193-194 https://doi.org/10.1071/AP02013
  20. Mordue, J. E. M. 1971. Colletotrichum gloeosporioides. CMI Descriptions of Pathogenic Fungi and Bacteria. No. 315. C.M.I. Kew, U.K
  21. Rohlf, E. J. 1993. NTSYS-pc: numerical taxonomy and multivariate analysis system, version 1.80. Applied Biostatistics Inc., Setauket, New York
  22. Simmonds, J. H. 1965. A study of the Colletotrichum causing ripe fruit rots in Queensland. Queensl. J. Agr. Anim. Sci. 22:437-459
  23. Southworth, E. A. 1891. Ripe rot of grapes and apples. J. Mycol. 6:64-173
  24. Sreenivasaprasad, S. and Talhinhas, P. 2005. Genotypic and phenotypic diversity in. Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Mol. Plant Pathol. 6:361-378 https://doi.org/10.1111/j.1364-3703.2005.00291.x
  25. Sutton, B. C. 1998. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. CMI, U.K. 696 pp
  26. Talhinhas, P. Sreenivasaprasad, S., Neves-Martins, J. and Oliveira, H. 2002. Genetic and morphological haracterization of Colletotrichum acutatum causing anthracnose of lupins. Phytopathology 92:986-996 https://doi.org/10.1094/PHYTO.2002.92.9.986
  27. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599 https://doi.org/10.1093/molbev/msm092
  28. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nuc. Acids Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  29. Whitelaw-Weckert, M. A., Curtin, S., Huang, R., Steel, C. C., Blanchard, C. L. and Roffey, P. 2007. Phylogenetic relationships and Pathogenicity of grape isolates of Colletotrichum acutatum in sub-tropical Austrialia. Australas. Plant Pathol. 56:448-463 https://doi.org/10.1111/j.1365-3059.2007.01569.x
  30. Yamamoto, J., Sato, T. and Tomioka, K. 1999. Occurrence of ripe rot of grapes (Vitis vinifera L.) caused by Colletotrichum acutatum Simmonds ex Simmonds. Ann. Phytopathol. Soc. Jpn. 65:83-86 https://doi.org/10.3186/jjphytopath.65.83

피인용 문헌

  1. Genetic Divesity Analysis of Fungal Species by Universal Rice Primer (URP)-PCR vol.40, pp.2, 2012, https://doi.org/10.4489/KJM.2012.40.2.78
  2. Identification and Characterization of the Causal Organism of Gummy Stem Blight in the Muskmelon (Cucumis meloL.) vol.38, pp.3, 2010, https://doi.org/10.4489/MYCO.2010.38.3.166
  3. Molecular and Pathological Characterization of Colletotrichum falcatum Infecting Subtropical Indian Sugarcane vol.159, pp.4, 2011, https://doi.org/10.1111/j.1439-0434.2010.01759.x
  4. Diverse species of Colletotrichum associated with grapevine anthracnose in China vol.71, pp.1, 2015, https://doi.org/10.1007/s13225-014-0310-9
  5. Identification and Characterization ofPseudocercosporaSpecies Causing Grapevine Leaf Spot in China vol.164, pp.2, 2016, https://doi.org/10.1111/jph.12427
  6. In vitro evaluation system for varietal resistance against ripe rot caused by Colletotrichum acutatum in grapevines vol.52, pp.1, 2011, https://doi.org/10.1007/s13580-011-0190-9
  7. Identification of Pathogens Causing Grape Rot on 'Red Glove' Variety Imported from Chile vol.18, pp.3, 2012, https://doi.org/10.5423/RPD.2012.18.3.217
  8. Ripe rot of south-eastern Australian wine grapes is caused by two species of Colletotrichum: C. acutatum and C. gloeosporioides with differences in infection and fungicide sensitivity vol.17, pp.2, 2011, https://doi.org/10.1111/j.1755-0238.2011.00143.x
  9. Market Disease Pathogens Detection of Imported Fruits in Shanghai vol.8, pp.9, 2009, https://doi.org/10.1016/S1671-2927(08)60316-8
  10. Grapevine Bunch Rots: Impacts on Wine Composition, Quality, and Potential Procedures for the Removal of Wine Faults vol.61, pp.22, 2013, https://doi.org/10.1021/jf400641r
  11. Colletotrichum species on grape in Guizhou and Yunnan provinces, China vol.54, pp.1, 2013, https://doi.org/10.1016/j.myc.2012.07.006
  12. spp. from Different Hosts in Shandong, China pp.0191-2917, 2018, https://doi.org/10.1094/PDIS-04-18-0597-RE
  13. Analysis of Varietal Difference and Genetic Diversity of Grapevine Cultivarsthrough the Leaf Inoculation of Colletotrichum spp. vol.52, pp.6, 2018, https://doi.org/10.14397/jals.2018.52.6.49
  14. species associated to ripe rot disease of grapes in the “Serra Gaucha” region of Southern Brazil vol.12, pp.2117-4458, 2019, https://doi.org/10.1051/bioconf/20191201008