한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제25권4호
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  • Pages.257-262
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  • 2008
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개에서 신장의 허혈-재관류 손상에 대한관류-흡인의 감소효과

Amelioration Effects of Irrigation-Aspiration on Renal Ischemia-Reperfusion Injury in Canine Model

  • 이재일 (충남대학교 수의과대학 수의외과학교실) ;
  • 손화영 (충남대학교 수의과대학 수의병리학교실) ;
  • 정성목 (충남대학교 수의과대학 수의외과학교실) ;
  • 김명철 (충남대학교 수의과대학 수의외과학교실)
  • Lee, Jae-Il (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University) ;
  • Son, Hwa-Young (Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Cheol (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2008.08.31
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초록

신장의 허혈-재관류 손상은 수술하는 동안 부득이하게 발생하는 허혈-재관류 손상에 대한 이식조직의 내성에 따라 이식된 장기의 생존력이 달려있기 때문에 임상적으로 아주 중요하다. 본 연구의 목적은 신장 혈관을 차단하여 유발한 허혈-재관류 모델에서 관류-흡인의 효과를 알아보고자 실시하였다. 신기능과 항산화 효소를 검사하기 위해 혈액 샘플을 채취하였고 신장내 동맥의 혈류저항을 측정하였다. 14일째 신장을 절제하여 조직검사를 실시하였다. 신기능(Cr, BUN)은 처치군에 비해 비처치군에서 유의성 있는 상승을 보였다. 신장내 혈류 저항은 두 그룹 사이에 유의성이 없었다. 항산화 효소 활성은 대조군에 비해 비처치군에서 유의성 있는 감소를 보였으나, 처치군에서는 대조군과 유의성이 없었다. 조직검사 결과에서도 처치군이 비처치군에 비해 적은 조직손상을 보였다. 이러한 결과는 관류-흡인 과정이 신장의 허혈-재관류 손상을 감소시키는데 유용한 단계임을 시사한다.

Renal ischemia-reperfusion injury is great clinical important because viability of the transplanted organ depends on the tolerance of the graft to ischemia-reperfusion injury, an inevitable processing during surgery. The purpose of this study was to investigate the effects of irrigation-aspiration in ischemia-reperfusion injury model induced by cross-clamping of renal vessels. Blood samples were collected from these dogs for measurement of kidney function and antioxidant enzyme activity, and RI at the intrarenal artery was measured at different time intervals. And the kidneys were taken for histopathologic evaluation at day 14. Kidney function (Cr and BUN) showed a significant increasing in untreated group compared to treated group. Resistive index of intrarenal artery was no significant difference among the groups. Activity of antioxidant enzymes in plasma was significant decrease in untreated group compare to control group while in treated group was no significant difference compared to control group. In histopathologic finding, treated group was showed less damage than that of untreated group. This result suggests that the processing of irrigation-aspiration is useful to reducing ischemia-reperfusion injury.

키워드

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