한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제25권4호
  • /
  • Pages.241-244
  • /
  • 2008
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개 혈액에서 Multiplex Nested PCR기법을 이용한 Brucella spp. 및 Leptospira interrogans 검출

Detection of Brucella spp. and Leptospira interrogans in the Canine Blood by Multiplex Nested PCR

  • 이정연 (충남대학교 동물의과학연구소.수의과대학) ;
  • 이상은 (충남대학교 동물의과학연구소.수의과대학) ;
  • 김석 (경상대학교 수의과대학) ;
  • 김덕환 (충남대학교 동물의과학연구소.수의과대학) ;
  • 송근호 (충남대학교 동물의과학연구소.수의과대학)
  • Lee, Jung-Youn (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Sang-Eun (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Duck-Hwan (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Song, Kun-Ho (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2008.08.31
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초록

본 연구는 특이적인 임상증상이 없는 360두의 개를 대상으로 multiplex nested PCR 기법을 이용하여 Brucella spp. 및 Leptospira interrogans를 검출하였다. Brucella spp.는 총4두 (1.1%, 암컷 2두, 수컷 2두)가 검출되었고, Leptospira interrogans는 59두 (16.4%, 암컷31두, 수컷28두)에서 검출되었다. 결론적으로 Brucella spp.의 검출률은 낮은 반면 Leptospira interrogans는 높았다. 또한 multiplex nested PCR기법은 무증상의 개 혈액에서 Brucella spp. 및 Leptospira interrogans를 조기에 검출하는데 빠르고 편리한 기법으로 판단된다.

This study examined the prevalence of Brucella spp. and Leptospira interrogans in 360 clinically healthy dogs using multiplex nested PCR. Four dogs (1.1%, 2 females and 2 males) tested positive to Brucella spp. by multiplex nested PCR. Fifty nine (16.4%, 31 females and 28 males) of 360 dogs tested positive L. interrogans. In 1 and 2 of the samples that tested positive to Brucella spp. and L. interrogans, the partial sequences of the virB1 and 16S rRNA genes were identified by direct sequence analysis, respectively. In conclusion, prevalence of Brucella spp. and L. interrogans by multiplex nested PCR revealed low and high, respectively. Multiplex nested PCR is can be useful for early detection of Brucella spp. and L. interrogans in the canine blood from asymptomatic dogs.

키워드

참고문헌

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