한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제23권3호
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  • Pages.161-166
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  • 2008
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

말 동결융해 정자의 생존성 및 수정능에 영향을 미치는 인자

Factors Affecting the Motility and Fertility of Frozen-thawed Stallion Semen

  • 발행 : 2008.09.30
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초록

The techniques for the collection, cooling and freezing of semen and artificial insemination of horses are not fully understood in Korea. We investigated the percentages of total motile (TM) and progressively motile (PM) sperms after the collection, cooling and freezing of stallion semen. The average volume of semen was 167 ml in Thoroughbred and 68 ml in Arab. The average numbers of spermatozoa in Thoroughbred and Arab were $104\times10^6/ml$ and $86\times10^6/ml$ respectively. The average percentages of TM and PM were 82.3% and 88.6% in Thoroughbred, and 61.4% and 82.6% in Arab, respectively. The average percentage of TM at 4 hr after cooling at $5^{\circ}C$ was significantly lower than that at 0 hr ($30.0\pm4.1%\;vs.\;78.0\pm2.5%,\;p<0.05$), but the percentage of PM was similar between 66.5 and 73.2% at 0, 1, and 4hr. The average percentage of frozen-thawed Thoroughbred semen frozen in MFR5 extender was 56.2%, which was significantly higher than that of the semen frozen in LE extender (average 32.9%, p<0.05). The percentage of TM in Arab was similar for semen frozen in MFR5 extender and LE extender (18.2% and 21.2%, respectively), but the percentage of PM was significantly higher in sperm frozen in MFR5 extender than in sperm frozen in LE extender (69.0% vs. 36.4%, p<0.05). Four mares were artificially inseminated by Thoroughbred frozen-thawed semen and one of them fertilized at 11 day after artificial insemination. In this study, the collection, cooling and freezing of equine semen were possible under domestic conditions.

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참고문헌

  1. Amann RP and Pickett BW. 1987. Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. J. Equine Vet. Sci. 7:145-173 https://doi.org/10.1016/S0737-0806(87)80025-4
  2. Backman T, Bruemmer JE, Graham JK and Squires EL. 2004. Pregnancy rates of mares inseminated with semen cooled for 18 hours and then frozen. J. Anim. Sci. 82:690-694
  3. Bedford SJ, Graham JK, Amann RP, Squires EL and Pickett BW. 1995. Use of two freezing extenders to cool stallion spermatozoa to 5'C with and without seminal plasma. Theriogenology 43:939-953 https://doi.org/10.1016/0093-691X(95)00044-9
  4. Buchanan BR, Seidel GE, McCue PM, Schenk JL, Herickhoff LA and Squires EL. 2000. Insemination of mares with low numbers of either unsexed or sexed spermatozoa. Theriogenology 53:1333-1344 https://doi.org/10.1016/S0093-691X(00)00276-4
  5. Cristanelli MJ, Squires EL, Amann RP and Pickett BW. 1984. Fertility of stallion semen processed, frozen and thawed by a new procedure. Theriogenology 22:39-45 https://doi.org/10.1016/0093-691X(84)90471-0
  6. Crockett EC, Graham JK, Bruemmer JE and Squires EL. 2001. Effect of cooling of equine spermatozoa before freezing on post-thaw motility: preliminary results. Theriogenology 55: 793-803 https://doi.org/10.1016/S0093-691X(01)00444-7
  7. Ecot P, Vidament M, deMornac A, Perigault K, Clement F and Palmer E. 2000. Freezing of stallion semen: interactions among cooling treatments, semen extenders and stallions. J. Reprod. Fertil. 56:141-150
  8. Hafez ESE and Hafez B. 2000. Reproduction in Farm Animals. 7th ed, Lippincott Williams & Wilkins, Maryland, pp. 382-385
  9. Heitland AV, Jasko DJ, Graham EL, Squires EL, Amann RP and Pickett BW. 1995. Motility and fertility of stallion spermatozoa cooled and frozen in a modified skim milk extender containing egg yolk and liposome. Biol. Reprod. Mongraph series 1:753-759
  10. Herman HA, Mitchell JR and Doak GA. 1994. The Artificial Insemination and Embryo Transfer of Dairy and Beef Cattle. 8th ed, Interstate publishers Inc, Illinois, pp. 66-67
  11. Jasko DJ, Hathaway JA, Schaltenbrand VL, Simper WD and Squires EL. 1992. Effect of seminal plasma and egg yolk on motion characteristics of cooled stallion spermatozoa. Theriogenology 37:1241-1252 https://doi.org/10.1016/0093-691X(92)90180-Y
  12. Loomis PR and Graham JK. 2008. Commercial semen freezing: Individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim. Reprod. Sci. 105:119-128 https://doi.org/10.1016/j.anireprosci.2007.11.010
  13. Loomis PR, Amann RP, Squires EL and Pickett BW. 1983. Fertility of unfrozen and frozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packaged in straws. J. Anim. Sci. 56:693-987
  14. Loomis PR. 2001. The equine frozen semen industry. Anim. Reprod. Sci. 68:191-200 https://doi.org/10.1016/S0378-4320(01)00156-7
  15. Martin JC, Klug E and Gunzel AR. 1979. Centrifugation of stallion spermatozoa and its storage in large volume straws. J. Reprod. Fertil. Suppl. 27:47-51
  16. Palmer E. 1984. Factors affecting stallion semen survival and fertility. Proce. 10th Cong. Anim. AI 3:377
  17. Samper JC and Morris CA. 1998. Current methods for stallion semen cryopreservation: a survey. Theriogenology 49:895-903 https://doi.org/10.1016/S0093-691X(98)00039-9
  18. Samper JC, Hearn PH and Ganheim A. 1994. Pregnancy rates and effect of extender and motility and acrosome status of frozen-thawed stallion spermatozoa. Of the 40th Ann. Conv. Amer. Assoc. Equine Pract. pp. 41-43
  19. Samper JC. 1995. Stallion semen cryopreservation: Male factors affecting pregnancy rates. Proc. Soc. Study Theriogenology 160-165
  20. Tischer M. 1979. Evaluation of deep-frozen semen in stallions. J. Reprod. Fert. Suppl. 27:53-59
  21. Vidament M, Dupere AM, Julienne P, Evain A, Noue P and Palmer E. 1997. Equine frozen semen freezability and fertility results. Theriogenology 48:907-917 https://doi.org/10.1016/S0093-691X(97)00319-1
  22. Woods J, Rigby S, Brinsko S, Stephens R, Varner D and Blanchard T. 2000. Effect of intrauterine treatment with prostaglandin $E_2$ prior to insemination of mares in the uterine horn or body. Theriogenology 53:1827-1836 https://doi.org/10.1016/S0093-691X(00)00318-6