대기노출형 백색부후균 생물막을 이용한 유기물 처리특성

Characteristics of Organics Treatment Using White-rot Fungus Biofilm of Atmospheric Exposed Type

  • Lee, Soon-Young (Jeonbuk Regional Environmental Technology Development Center) ;
  • Kang, Ki-Cheol (Department of Environmental Engineering, Chonbuk National University) ;
  • Won, Chan-Hi (Department of Environmental Engineering, Chonbuk National University)
  • 발행 : 2008.05.31

초록

점점 더 강화되는 수질기준, 연료 및 에너지비용 증가 등으로 경제성, 소요부지의 최소화, 운전의 용이성, 슬러지 발생의 최소화, 높은 처리효율을 지니는 폐수처리시스템의 개발은 시급한 실정이다. 따라서 본 연구에서는 난분해성물질을 함유한 오 폐수에 대해 대기노출형 백색부후균 생물막을 이용하여 생물막의 침지형태, 체류시간, 재순환비, 모듈회전수에 따른 유기물 처리 특성을 연구하였다. HBC 링 여재에 부착된 백색부후균 생물막을 대기중에 완전히 노출하여 운전시 침지형 포기조건과 거의 비슷한 제거율을 나타냈다. 대기노출형의 최적조건은 HRT 3$\sim$4 hr, 재순환비 6$\sim$10 Q, 모듈회전수 0.5$\sim$2회/min이며, 이때 유기물 제거율은 COD$_{Cr}$ 65.0$\sim$69.9%, NBDCOD 70.4$\sim$72.7%, BOD$_5$ 88.8$\sim$90.1%, SS 84.2$\sim$90.4%를 나타냈다. 또한 본 연구에서 BOD$_5$의 유출수 평균농도는 8.9 mg/L로 중수도 수질기준 BOD$_5$ 10 mg/L이하를 만족하였으나, NBDCOD의 경우 평균농도가 29.6 mg/L로 중수도 수질기준 20 mg/L보다 높은 것으로 나타났다.

It is really urgent to develop wastewater treatment system which is economically efficient, occupies small area for buildup, can be easily operated, discharges small amount of sludge due to the more strict water quality standard, the expensive water and energy cost and so on. This study on treatment of wastewater including nonbiodegadable materials using white-rot fungus biofilm were designed to investigate the submerged type of biofilm, hydraulic retention times, recycle rates, and module turning times. Removal efficiencies of fully exposed biofilm type in atmosphere are similar to submerged biofilm of aeration type. The optimum conditions of white-rot fungus biofilm of fully exposed type in atmosphere are HRT 3$\sim$4 hr, recycle rate 6$\sim$10 Q, module turning times 0.5$\sim$2 times/min. At this time, removal efficiencies of organics were COD$_{Cr}$ 65.0$\sim$69.9%, NBDCOD 70.4$\sim$72.7%, BOD$_5$ 88.8$\sim$90.1%, SS 84.2$\sim$90.4%. Moreover average effluent concentration of BOD$_5$(8.9 mg/L) satisfied water quality standard of heavy water(BOD$_5$ less than 10 mg/L) but concentration of NBDCOD(29.6 mg/L) was higher than water quality standard of heavy water(NBDCOD less than 20 mg/L).

키워드

참고문헌

  1. Deguchi, T., Kakezawa, M., and Nishida, T., "Nylon biodegradation by lignnin-degrading fungi," Appl. Environ. Microbiol., 63, 329-331(1998)
  2. Eggen, T., "Application of fungal substrate from commercial mushroom production Pleurotus ostreatus for bioremediation of creosote contaminated soil," Biodegradation, 44, 117-126(1999)
  3. Maria, T. M., Ivan, Gumersindo, F., "Evaluation of different fungal strains in the decolourisation of synthetic dyes," Kluwer Academic Publishers, 22, 1499-1503(2000) https://doi.org/10.1023/A:1005606330152
  4. Semple, K. T., Reid, B. J., and Fermor, T. R., "Impact of composing strategies on the treatment of soils contaminated with organic pollutants," Environ. Pollut., 112, 269-283(2001) https://doi.org/10.1016/S0269-7491(00)00099-3
  5. Webb, M. D., Ewbank, G., Perkings, J., and McCarthy, A. J., "Metabolism of pentachlorophenol by Saccharomonospora biridis strains isolated from mushroom compost," Soil. Biol, Biochem., 33, 1903-1914(2001) https://doi.org/10.1016/S0038-0717(01)00115-8
  6. Lau, K. L., Tsang, Y. Y., and Chiu, S. W., "Use of spent mushroom compost to bioremediate PAH-contaminated samples," Chemosphere, 52, 1539-1546(2003) https://doi.org/10.1016/S0045-6535(03)00493-4
  7. Law, W. M., Lau, W. N., Lo, K. L., Wai, L. M., and Chiu, S. W., "Removal of biocide pentachlorophenol in water system by the spent mushroom compost of Pleurotus pulmonatius," Chemosphere, 52, 1531-1537(2003) https://doi.org/10.1016/S0045-6535(03)00492-2
  8. Xawed, V., Bhatt, T., Cajthami, T., Malachobá, K., and Lednicka, D., "Compost-mediated remobal of polycyclic aromatic hydrocarbons from contaminated soil," Arch Environ. Contam. Toxicol., 44, 336-342(2003) https://doi.org/10.1007/s00244-002-2037-y
  9. Watanabe, K., "Microorganisms relevant to bioremediation," Current Opinion in Biotechnology, 12(3) 237-241 (2001) https://doi.org/10.1016/S0958-1669(00)00205-6
  10. 류원률, 백색부후균에 의한 Ligninase의 생산 및 Xenobiotics의 분해 특성에 관한 연구, 영남대학교 대학원 응용화학공학과 유기공업화학 및 생물화학공학 전공, 박사학위논문, pp. 95-100(2001)
  11. Leisola, M., Thanei-Wyss, U., and Fiechter, A., "Strategies for Production of High Ligninase Activities by Phanerochaete chrysosporium," J. Biotechnol., 3, 97-107(1985) https://doi.org/10.1016/0168-1656(85)90010-0
  12. Moreira, M. T., Feijoo, G., Palma, C., Lema, J. M., "Continuous production of Manganese Peroxidase by Phanerochaete chrysosporium immobilized on polyurethane foam in a pulsed packed-bed bioreactor," Biotechnol. Bioeng., 56, 130-137(1997) https://doi.org/10.1002/(SICI)1097-0290(19971020)56:2<130::AID-BIT2>3.0.CO;2-Q
  13. Cornwell, K. L., Tinland-Butez, M. F., Tardone, P. J., Cabasso, I., Hammel, K. E., "Lignin degradation and lignin peroxidase production in cultures of Phanerochaete chrysosporium immobilized on porous ceramic supports," Enzyme Microb. Technol., 12, 916-920(1990) https://doi.org/10.1016/0141-0229(90)90109-4
  14. Hela Zouari, Marc Labat, Sami Sayadi, "Degradation of 4-chlorophenol by the white rot fungus Phanerochaete chrysosporium in free and immobilized cultures," Bioresour. Technol., 84, 145-150(2002) https://doi.org/10.1016/S0960-8524(02)00032-9
  15. Soon-Seop Shim, Katsuya Kawamoto, "Enzyme production activity of Phanerochaete chrysosporium and degradation of pentachlorophenol in a bioreactor," Water Res., 36, 4445-4454(2002) https://doi.org/10.1016/S0043-1354(02)00179-3
  16. Sheldon, M. S., Small, H. J., "Immobilisation and biofilm development of Phanerochaete chrysosporium on polysulphone and ceramic membranes," J. Membr. Sci., 263, 30-37(2005) https://doi.org/10.1016/j.memsci.2005.04.014
  17. Oh, Y. S., Choi, S. C., and Kim, Y. K., "Degradation of Gaseous BTX by Biofiltration with Phanerochaete chrysosporium," The Journal of Microbiol., 36(1), 34-38(1998)
  18. Juan Wu, Ya-Zhong Xiao and Han-Qing Yu, "Degradation of lignin in pulp mill wastewaters by white-rot fungi on biofilm," Bioresour. Technol., 96(12). 1357-1363(2005) https://doi.org/10.1016/j.biortech.2004.11.019
  19. Yongmin Zhang, Bruce E. Rittmann, Jianlong Wang, Yuhong Sheng, Juntang Yu, Hanchang Shi, Yi Qian, "High-carbohydrate wastewater treatment by IAL-CHS with immobilized Candida tropicalis," Process Biochem., 40, 857-863(2005) https://doi.org/10.1016/j.procbio.2004.02.010
  20. Field, J. A., E. de Jong, G. F. Costa, and J. de Bont, "Biodegradation of by New Isolates of White Rot Fungi," Appl. Environ. Microbiol., 58, 2219-2226(1992)
  21. Ibrahim, M. B., Poonam, N., Datel, S., Roger, M., "Microbial decolorization of textile-dye-containing effluents: A review," Bioresour. Technol., 58, 217-227(1996) https://doi.org/10.1016/S0960-8524(96)00113-7
  22. Tien, M., Kirk, T. K., "Selection and Improvement of Lignin-Degrading Microorganisms," Appl. Environ. Microbiol., 53(2), 242-245(1987)
  23. 유재익, "회전원판법에 의한 하수처리에 관한 연구," 동아대학교, 석사학위논문, p. 16(1985)
  24. Egemen, E., Corpening, J., and Nirmalakhandan, N., "Evaluation of an ozonation system for reduced waste sludge generation," Water Sci. Technol., 44(2-3), 445-450(2001) https://doi.org/10.2166/wst.2001.0800
  25. 이동운, "미생물 접촉여재를 이용한 생물막공정의 슬러지 원천감량에 관한 연구," 계명대학교, 박사학위논문, p. 98(2007)