호알칼리성, 고온성 Bacillus cereus TA-11으로 생산된 세포내 Invertase의 생합성 조절

Biosynthetic Regulation of Intracellular Invertase from Alkalophilic and Thermoplilic Bacillus cereus TA-11

호 알칼리성이며 고온성인 Bacillus cereus TA-11이 세포내로 생성하는 invertase의 생합성 조절 양상을 조사하였다. Bacillus cereus TA-11의 세포내 invertase는 10 mM sucrose의 180분 처리와 25 mM raffinose의 90분 처리에서 각각 효율적으로 유도되었다. 또한 glucose는 sucrose에 의한 invertase의 유도를 억제하였고 cAMP첨가는 catabolite repression을 감소시키지 못하였다.

Regulation of invertase biosynthesis was studied with alkalophilic and thermophilic Bacillus cereus TA-11. Biosynthesis of invertase in Bacillus cereus TA-11 was effectively induced in the presence of 10 mM of sucrose for 180 min and 25 mM of raffinose for 90 min, respectively. Glucose repressed the invertase induction by sucrose and as late addition time of glucose, invertase formation was increased, indicating that glucose repression was occurred by inducer exclusion. Catabolite repression was not reduced by the addition of cAMP for 180 min of induction.