Effects of acupuncture at the $LU8{\cdot}K17$ on Anti-apoptotic cell death and neuroprotection in Rat hippocampus following focal brain ischemic injury induced by Intraluminal Filament insertion in Rats

직자(直刺), 영수(迎隨) 및 염전수기법(捻轉手技法)에 따라 시행한 경거(經渠)${\cdot}$복류(復溜) 침자(鍼刺)가 중대뇌동맥(中大腦動脈) 폐새(閉塞)에 의하여 유발(誘發)된 국소(局所) 뇌허혈(腦虛血) 백서(白鼠) hippocampus의 항세포자멸사(抗細胞自滅死) 및 복경보호(福經保護)에 미치는 영향(影響)

  • Youn, Dae-Hwan (College of Oriental Medicine, Dongshin University) ;
  • Byun, Jeng-Yun (College of Oriental Medicine, Dongshin University) ;
  • Choi, Chan-Hun (College of Oriental Medicine, Dongshin University) ;
  • Baek, Jin-Ung (College of Oriental Medicine, Dongshin University) ;
  • Jeong, Ji-Yeon (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
  • Jung, Yeon-Jin (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
  • Na, Chang-Su (College of Oriental Medicine, Dongshin University)
  • 윤대환 (동신대학교 한의과대학) ;
  • 변정윤 (동신대학교 한의과대학) ;
  • 최찬헌 (동신대학교 한의과대학) ;
  • 백진웅 (동신대학교 한의과대학) ;
  • 정지연 (전남대학교 치의학전문대학원, 치의학연구소) ;
  • 정연진 (전남대학교 치의학전문대학원, 치의학연구소) ;
  • 나창수 (동신대학교 한의과대학)
  • Published : 2007.09.27

Abstract

Objectives : Aims of this study is to investigate the effects of $LU8{\cdot}KI7$ in rat induced by experimental focal ischemia. Materials and methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The groups divided into 6groups, control; no therapy group after ischemia-induced, AT1; acupuncture therapy group at $LU8{\cdot}KI7$ after ischemia-induced, AT2; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the thumb of the right hand 9times, AT3; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally and the needle was twirled and rotated forward with the forefinger of the right hand 9times, AT4; acupuncture therapy at$LU8{\cdot}KI7$ bilaterally and the needle was inserted to the direction following the flowing route of the meridian(digital direction), AT5; acupuncture therapy at $LU8{\cdot}KI7$ bilaterally, the needle was inserted to the direction following the flowing route of the meridian(digital direction) and the needle was twirled and rotated forward with the thumb of the right hand 9times. Acupuncture therapy was carried out 7times during 2weeks after focal ischemia-induced. The anti-apoptotic and neuroprotective effects of acupuncture are observed by Bax, Bcl-2, mGluR5, cytochrome c, Cresyl violet and ChAT-stain. Results : The intensity of Bax was decreased in AC1, AC4, AC5 group, was increased in AC2, AC3 group. The intensity of Bcl-2 was increased in AC2, AC3, AC4, AC5 group. The intensity of mGluR5 was decreased in AC1 group, was increased in AC4, AC5 group. The intensity of Cytochrome c was increased in ACI, AC2 group, was decreased in AC4, AC5 group. The density of neurons stained by Cresyl violet was increased in all group without control group. The density of ChAT was increased in AC2, ACS group. Conclusions : Our study suggests that AC5 group show anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.

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