The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Neuroprotective Effect of Cirsium japonicum and Silibinin on Lipopolysaccharide-induced Inflammation in BV2 Microglial Cells

대계와 실리비닌의 Mouse BV2 Microglial Cells에서 Lipopolysaccharide에 의해 유발된 염증반응에 대한 신경보호 효과

  • Yeo, Hyun-Soo (Department of Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Kim, Dong-Woo (Department of Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Jun, Chan-Yong (Department of Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Choi, You-Kyung (Department of Internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Park, Chong-Hyeong (Department of Internal Medicine, College of Oriental Medicine, Kyungwon University)
  • 여현수 (경원대학교 한의과대학 내과학교실) ;
  • 김동우 (경원대학교 한의과대학 내과학교실) ;
  • 전찬용 (경원대학교 한의과대학 내과학교실) ;
  • 최유경 (경원대학교 한의과대학 내과학교실) ;
  • 박종형 (경원대학교 한의과대학 내과학교실)
  • Published : 2007.03.31
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Abstract

Objectives : This study was designed to evaluate the neuroprotective effect of Cirsium japonicum and Silibinin on lipopolysaccharide-induced inflammation in BV2 microglial cells. Methods : We studied on the neuroprotective effect of lipopolysaccharide-induced inflammation using MTS assay, western blot, and nitric oxide detection on mouse BV2 microglial cells. Results : Cirsium japonicum dose-dependently (50${\mu}g/ml$${\sim}$$250{\mu}g/ml$) inhibited nitrite production and iNOS expression in lipopolysaccharide-induced BV2 microglia and also significantly reduced lipopolysaccharide-induced COX-2 activation in western blot. Silibinin dose-dependently (10${\mu}M$${\sim}$$100{\mu}M$) inhibited nitrite production and iNOS expression in lipopolysaccharide-induced BV2 microglial cells. Silibinin also significantly reduced lipopolysaccharide-induced COX-2 activation in western blot. Conclusion : These effects of neuroprotection related to anti-inflammation suggest that Cirsium japonicum and Silibininmay be useful candidates for the development of a drug for related neurodegenerative diseases.

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